Recombinant Human PDGF-AA Protein, CF

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Recombinant Human PDGF-AA 221-AA stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 50-200 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human PDGF-AA Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002).
Source
E. coli-derived human PDGF-AA protein
Ser87-Thr211, with and without an N-terminal Met
Accession #
N-terminal Sequence
Ser87 and Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
PDGFA
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
14 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
221-AA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF-AA Protein, CF

  • PDGF1
  • PDGF-A
  • PDGFAA
  • PDGF-AA

Background

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta . Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

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Publications for PDGF-AA (221-AA)(48)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Primate.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Surface Plasmon Resonance.


Filter By Application
Bioassay
(39)
Cell Culture
(5)
ELISA (Standard)
(1)
In Vivo
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(29)
Mouse
(10)
Rat
(6)
N/A
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using 221-AA Applications Species
JO Kim, SE Baek, EY Jeon, JM Choi, EJ Jang, CD Kim PDGFR-beta signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells PLoS ONE, 2022;17(3):e0265191. 2022 [PMID: 35294955] (Cell Culture, Rat) Cell Culture Rat
K Moore, D Fulmer, L Guo, N Koren, J Glover, R Moore, C Gensemer, T Beck, J Morningsta, R Stairley, RA Norris PDGFR&amp;alpha: Expression and Function during Mitral Valve Morphogenesis Journal of cardiovascular development and disease, 2021;8(3):. 2021 [PMID: 33805717] (Bioassay, Human) Bioassay Human
X Chamling, A Kallman, W Fang, CA Berlinicke, JL Mertz, P Devkota, IEM Pantoja, MD Smith, Z Ji, C Chang, A Kaushik, L Chen, KA Whartenby, PA Calabresi, HQ Mao, H Ji, TH Wang, DJ Zack Single-cell transcriptomic reveals molecular diversity and developmental heterogeneity of human stem cell-derived oligodendrocyte lineage cells Nature Communications, 2021;12(1):652. 2021 [PMID: 33510160] (Bioassay, Human) Bioassay Human
C Wang, M Zhang, G Garcia, E Tian, Q Cui, X Chen, G Sun, J Wang, V Arumugaswa, Y Shi ApoE-Isoform-Dependent SARS-CoV-2 Neurotropism and Cellular Response Cell Stem Cell, 2021;28(2):331-342.e5. 2021 [PMID: 33450186] (Bioassay, Human) Bioassay Human
Y Yamamoto, A Mukai, T Ikushima, Y Urata, S Kinoshita, J Hamuro, M Ueno, C Sotozono Pluripotent epigenetic regulator OBP-801 maintains filtering blebs in glaucoma filtration surgery model Scientific Reports, 2020;10(1):20936. 2020 [PMID: 33262357] (Cell Culture, Human) Cell Culture Human
KC Allan, LR Hu, MA Scavuzzo, AR Morton, AS Gevorgyan, EF Cohn, BLL Clayton, IR Bederman, S Hung, CF Bartels, M Madhavan, PJ Tesar Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33091368] (Bioassay, Mouse) Bioassay Mouse
K Song, Y Qing, Q Guo, EK Peden, C Chen, WE Mitch, L Truong, J Cheng PDGFRA in vascular adventitial MSCs promotes neointima formation in arteriovenous fistula in chronic kidney disease JCI Insight, 2020;0(0):. 2020 [PMID: 33001865] (Bioassay, Mouse) Bioassay Mouse
J Lim, J Heo, H Ju, JW Shin, Y Kim, S Lee, HY Yu, CM Ryu, H Yun, S Song, KS Hong, HM Chung, HR Kim, JS Roe, K Choi, IG Kim, EM Jeong, DM Shin Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway Sci Adv, 2020;6(16):eaba1334. 2020 [PMID: 32490200] (Bioassay, Human) Bioassay Human
A Yoshida, K Takashima, T Shimonaga, M Kadokura, S Nagase, S Koda Establishment of a simple one-step method for oligodendrocyte progenitor cell preparation from rodent brains J. Neurosci. Methods, 2020;0(0):108798. 2020 [PMID: 32479973] (Cell Culture, Rat) Cell Culture Rat
H Patel, J Li, A Herrero, J Kroboth, A Byron, AV Kriegsheim, V Brunton, N Carragher, T Hurd, M Frame Novel roles of PRK1 and PRK2 in cilia and cancer biology Sci Rep, 2020;10(1):3902. 2020 [PMID: 32127582] (Bioassay, Mouse) Bioassay Mouse
Show All 48 Publications.

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Bioinformatics

Gene Symbol PDGFA
Uniprot