Recombinant Human PDGF-AA Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human PDGF-AA Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002).
Source
E. coli-derived human PDGF-AA protein
Ser87-Thr211, with and without an N-terminal Met
Accession #
N-terminal Sequence
Ser87 and Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
PDGFA
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
221-AA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF-AA Protein, CF

  • PDGFAA
  • PDGF-AA

Background

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Publications for PDGF-AA (221-AA)(31)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Primate.

We have publications tested in 4 applications: Bioassay, ELISA Standard, In Vivo, Surface Plasmon Resonance.


Filter By Application
Bioassay
(27)
ELISA Standard
(1)
In Vivo
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(18)
Mouse
(6)
Rat
(4)
N/A
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using 221-AA Applications Species
CC Cheng, PN Liao, AS Ho, KH Lim, J Chang, YW Su, CG Chen, YW Chiang, BL Yang, HC Lin, YC Chang, CC Chang, YF Chang STAT3 exacerbates survival of cancer stem-like tumorspheres in EGFR-positive colorectal cancers: RNAseq analysis and therapeutic screening J. Biomed. Sci., 2018;25(1):60. 2018 [PMID: 30068339] (Bioassay, Human) Bioassay Human
P Piñol-Jura, X Suárez-Cal, E Fernández-, E Gallardo, N de la Oliv, A Martínez-M, P Gómez-Gálv, LM Escudero, M Pérez-Peir, L Wollin, N de Luna, X Navarro, I Illa, J Díaz-Maner Nintedanib decreases muscle fibrosis and improves muscle function in a murine model of dystrophinopathy Cell Death Dis, 2018;9(7):776. 2018 [PMID: 29991677] (Bioassay, Human) Bioassay Human
P Göttle, A Manousi, D Kremer, L Reiche, HP Hartung, P Küry Teriflunomide promotes oligodendroglial differentiation and myelination J Neuroinflammation, 2018;15(1):76. 2018 [PMID: 29534752] (Bioassay, Rat) Bioassay Rat
SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics Sci Rep, 2017;7(1):16439. 2017 [PMID: 29180757] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
A Campbell, JM Hogestyn, CJ Folts, B Lopez, C Pröschel, D Mock, M Mayer-Prös Expression of the Human Herpesvirus 6A Latency-Associated Transcript U94A Disrupts Human Oligodendrocyte Progenitor Migration Sci Rep, 2017;7(1):3978. 2017 [PMID: 28638124] (Bioassay, Human) Bioassay Human
R Santos, KC Vadodaria, BN Jaeger, A Mei, S Lefcochilo, APD Mendes, G Erikson, M Shokhirev, L Randolph-M, C Fredlender, S Dave, R Oefner, C Fitzpatric, M Pena, JJ Barron, M Ku, AM Denli, BE Kerman, P Charnay, JR Kelsoe, MC Marchetto, FH Gage Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells Stem Cell Reports, 2017;8(6):1757-1769. 2017 [PMID: 28591655] (Bioassay, Human) Bioassay Human
Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry PLoS Pathog., 2017;13(4):e1006281. 2017 [PMID: 28403202] (Bioassay, Human) Bioassay Human
Nevin Z, Factor D, Karl R, Douvaras P, Laukka J, Windrem M, Goldman S, Fossati V, Hobson G, Tesar P Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes. Am J Hum Genet, 0;100(4):617-634. 0 [PMID: 28366443] (Bioassay, Human) Bioassay Human
ZS Nevin, DC Factor, RT Karl, P Douvaras, J Laukka, MS Windrem, SA Goldman, V Fossati, GM Hobson, PJ Tesar Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes Am. J. Hum. Genet., 2017;100(4):617-634. 2017 [PMID: 28366443] (Bioassay, Human) Bioassay Human
H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3 Sci Rep, 2017;7(0):43736. 2017 [PMID: 28252047] (Bioassay, Human) Bioassay Human
Show All 31 Publications.

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Bioinformatics

Gene Symbol PDGFA
Uniprot