PCNA Antibody (PC10) - Azide and BSA Free

Images

 
Western Blot: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF ...read more
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA ...read more
Immunohistochemistry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization. Image from the standard format of this antibody.
Flow Cytometry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were ...read more
Western Blot: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts. Image from the standard format ...read more
Western Blot: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - HeLa cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins ...read more
Immunohistochemistry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and ...read more
Simple Western: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Dr, Fi, Mar, Pm, Rb, Ye, ZeSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IP, ChIP, IHC
Clone
PC10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

PCNA Antibody (PC10) - Azide and BSA Free Summary

Description
Novus Biologicals Mouse PCNA Antibody (PC10) - Azide and BSA Free (NB500-106) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF, Simple Western, IP and ChIP. Anti-PCNA Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
Protein A-rat PCNA fusion obtained from pC2T
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PCNA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 26883631)
  • ELISA reported in scientific literature
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:2000. Use reported in scientific literature (PMID 22068968)
  • Immunohistochemistry Free-Floating reported in scientific literature (PMID 27212918)
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
  • Simple Western 1:200
  • Western Blot 1:2000
Application Notes
It detects a band at 30 kDa by Western blot. For IHC on paraffin sections, heat-mediated citrate buffer antigen retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:200. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-80905 in the following applications:

  • WB
    1 publication

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 30075024).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15M NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for PCNA Antibody (PC10) - Azide and BSA Free

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • PCNA
  • proliferating cell nuclear antigen

Background

PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IP, ChIP, IHC

Publications for PCNA Antibody (NBP2-80905)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PCNA Antibody (NBP2-80905) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for PCNA Antibody (NBP2-80905)

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Blogs on PCNA.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and ...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and ...  Read full blog post.

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Bioinformatics

Gene Symbol PCNA