PCNA Antibody (PC10) - Azide and BSA Free

Western Blot: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.

Immunohistochemistry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization. Image from the standard format of this antibody.

Flow Cytometry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to DyLight 488.

Western Blot: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts. Image from the standard format of this antibody.

Western Blot: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - HeLa cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Image from the standard format of this antibody.

Immunohistochemistry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by Dr. Siegenthaler via product review. Image from the standard format of this antibody.

Simple Western: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Rt, Po, Ch, Dr, Fi, Mar, Pm, Rb, Ye, Ze
• Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IP, ChIP, IHC
• Clone: PC10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

PCNA Antibody (PC10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PCNA Antibody (PC10) - Azide and BSA Free (NB500-106) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF, Simple Western, IP and ChIP. Anti-PCNA Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Protein A-rat PCNA fusion obtained from pC2T
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCNA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 26883631)
  • ELISA reported in scientific literature
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:2000. Use reported in scientific literature (PMID 22068968)
  • Immunohistochemistry Free-Floating reported in scientific literature (PMID 27212918)
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
  • Simple Western 1:200
  • Western Blot 1:2000
• Application Notes: It detects a band at 30 kDa by Western blot. For IHC on paraffin sections, heat-mediated citrate buffer antigen retrieval is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:200. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 30075024).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PCNA Antibody (PC10) - Azide and BSA Free

• cyclin
• DNA polymerase delta auxiliary protein
• MGC8367
• PCNA
• proliferating cell nuclear antigen

Background

PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PCNA Antibody (NBP2-80905)(1)

Publication using NBP2-80905

• Lundine D, Annor GK, Chavez V et al. The C-terminus of Gain-of-Function Mutant p53 R273H Is Required for Association with PARP1 and Poly-ADP-Ribose Molecular cancer research : MCR 2022-12-02 [PMID: 36074101] (WB, Human)

Bioinformatics

• Gene Symbol: PCNA