PCNA Antibody (PC10) [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, ZeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
PC10
Clonality
Monoclonal
Host
Mouse

PCNA Antibody (PC10) [DyLight 550] Summary

Immunogen
Protein A-rat PCNA fusion obtained from pC2T
Marker
Proliferation Marker
Specificity
Specific for PCNA p36 protein expressed at high levels in proliferating cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PCNA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 550
Format
DyLight 550 (A=562, E=576)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:10-1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:5001:1000
  • Immunoprecipitation 1:100
Application Notes
This PCNA (PC10) antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation, Immunocytochemistry/Immunofluorescence (PMID: 22068968) Flow Cytometry and Western blot. It detects a band at 30 kDa by Western blot. For IHC on paraffin sections heat-mediated citrate buffer antigen retrieval is recommended.ELISA was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Yeast (S. pombe and S. cerevisiae), Drosophila melanogaster, Zebrafish, Rat, Mouse, Monkey, Chicken, Rabbit and Fish.

Alternate Names for PCNA Antibody (PC10) [DyLight 550]

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • proliferating cell nuclear antigen

Background

PCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumour tissue (i.e. relevant to prognosis).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PCNA Antibody (NB500-106R) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB500-106APC
DyLight 350 Labeled NB500-106UV
DyLight 405 Labeled NB500-106V
DyLight 405LS Labeled NB500-106V3
DyLight 488 Labeled NB500-106G
DyLight 650 Labeled NB500-106C
DyLight 680 Labeled NB500-106FR
DyLight 755 Labeled NB500-106IR
FITC Labeled NB500-106F
HRP Labeled NB500-106H
PE Labeled NB500-106PE
PerCP Labeled NB500-106PCP

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Blogs on PCNA.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol PCNA

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