CDK4 Antibody


Western Blot: CDK4 Antibody [NBP1-31308] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG more
Immunocytochemistry/ Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a more
Immunohistochemistry-Paraffin: CDK4 Antibody [NBP1-31308] - Human gastric cancer, using CDK4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody more
Genetic Strategies: Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 more
Immunocytochemistry/ Immunofluorescence: CDK4 Antibody [NBP1-31308] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.

Product Details

Reactivity Hu, Mu, Rt, Bv, Fe, Po, RM, ShSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Validated by:

Genetic Strategies


Order Details

CDK4 Antibody Summary

Recombinant protein encompassing a sequence within the center region of human CDK4. The exact sequence is proprietary.
Predicted Species
Porcine (98%), Rhesus Macaque (100%), Sheep (95%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Simple Western 1:50
  • Western Blot 1:500-1:3000
Application Notes
Simple Western reported by an internal validation. Separated by Size, antibody dilution of 1:50, detects a band at 38 kDa.
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP1-31308 in the following applications:

  • 1 publication
  • 1 publication
  • WB
    4 publications

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID:31987327). Feline reactivity reported in scientific literature (PMID:27830988).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 20% Glycerol (pH7)
0.025% Proclin 300
Immunogen affinity purified

Alternate Names for CDK4 Antibody

  • CDK4
  • Cell division protein kinase 4
  • CMM3
  • cyclin-dependent kinase 4
  • EC 2.7.11
  • EC
  • melanoma cutaneous malignant, 3
  • MGC14458
  • PSK-J3
  • PSK-J3cell division kinase 4


The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDK4 Antibody (NBP1-31308)(7)

We have publications tested in 3 confirmed species: Human, Bovine, Feline.

We have publications tested in 3 applications: Flow, IHC-P, WB.

Filter By Application
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All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-31308 Applications Species
Liu B, Fu C, Cao J Et al. Proliferation of bovine endometrial epithelial cells is promoted by prostaglandin E2-PTGER2 signaling through cell cycle regulation Prostaglandins, Leukotrienes and Essential Fatty Acids Nov 1 2021 [PMID: 34740034] (WB, Bovine) WB Bovine
Chen BR, Wang Y, Tubbs A et al. LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells eLife Sep 3 2021 [PMID: 34477552] (WB) WB
Contractor T, Harris C Loss of copy of MIR1-2 increases CDK4 expression in ileal neuroendocrine tumors. Oncogenesis Mar 20 2020 [PMID: 32198354] (Flow, Human) Flow Human
Fu C, Mao W, Gao R et al. Prostaglandin F2 alpha-PTGFR signaling promotes proliferation of endometrial epithelial cells of cattle through cell cycle regulation Anim. Reprod. Sci. Feb 1 2020 [PMID: 31987327] (WB, Bovine) WB Bovine
Isohookana J, Haapasaari KM, Soini Y et al. Proteins of the retinoblastoma pathway, FEN1 and MGMT are novel potential prognostic biomarkers in pancreatic adenocarcinoma. Pathol Res Pract 2018 Jun [PMID: 29735403] (Human) Human
Poncelet L, Garigliany M, Ando K et al. Cell cycle S phase markers are expressed in cerebral neuron nuclei of cats infected by the Feline Panleukopenia Virus Cell Cycle. 2016 Nov 09 [PMID: 27830988] (IHC-P, Feline) IHC-P Feline
Huang AM, Kao YT, Toh S et al. UBE2M-mediated p27(kip1) degradation in gemcitabine cytotoxicity. Biochem Pharmacol 2011 Jul [PMID: 21477582] (WB, Human) WB Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Gene Symbol CDK4