CDK2 Antibody (OTI2D9)

Images

 
Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.
Immunohistochemistry-Paraffin: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.
Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cdk2 antibody, and then analyzed by ...read more
Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Immunofluorescent staining of HeLa cells using anti-Cdk2 mouse monoclonal antibody.
Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - Analysis of Hela cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - Analysis of Jurkat cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI2D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.78 mg/ml

Order Details

CDK2 Antibody (OTI2D9) Summary

Immunogen
Full length human recombinant protein of human CDK2 (NP_001789) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CDK2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CDK2 Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP2-00776 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.78 mg/ml
Purity
Affinity purified

Alternate Names for CDK2 Antibody (OTI2D9)

  • cdc2-related protein kinase
  • CDK2
  • cell devision kinase 2
  • Cell division protein kinase 2
  • cyclin-dependent kinase 2
  • EC 2.7.11
  • EC 2.7.11.22
  • p33 protein kinase
  • p33(CDK2)

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDK2 Antibody (NBP2-00776) (0)

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Review for CDK2 Antibody (NBP2-00776) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-00776:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot CDK2 NBP2-00776
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reviewed by:
Anonymous
WB Human 08/08/2014
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Summary

ApplicationWestern Blot
Sample TestedNuclear and cytosolic lysates from 293T cells
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 2 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CDK2 Antibody (NBP2-00776) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70392AF350
Alexa Fluor 405 NBP2-70392AF405
Alexa Fluor 488 NBP2-70392AF488
Alexa Fluor 532 NBP2-70392AF532
Alexa Fluor 594 NBP2-70392AF594
Alexa Fluor 647 NBP2-70392AF647
Alexa Fluor 700 NBP2-70392AF700
Alexa Fluor 750 NBP2-70392AF750
Allophycocyanin NBP2-70392APC
Biotin NBP2-70392B
DyLight 350 NBP2-70392UV
DyLight 405 NBP2-70392V
DyLight 488 NBP2-70392G
DyLight 550 NBP2-70392R
DyLight 594 NBP2-70392DL594
DyLight 650 NBP2-70392C
DyLight 680 NBP2-70392FR
DyLight 755 NBP2-70392IR
FITC NBP2-70392F
HRP NBP2-70392H
Janelia Fluor 549 NBP2-70392JF549
Janelia Fluor 646 NBP2-70392JF646
PE NBP2-70392PE
PerCP NBP2-70392PCP

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Recent Reviews

5
5
1
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0
2
0
1
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Anonymous
08/08/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDK2
Entrez