p27/Kip1 [p Thr157] Antibody

Images

 
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291‑G1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

p27/Kip1 [p Thr157] Antibody Summary

Immunogen
Phosphopeptide containing human p27/Kip1 T157 site
Modification
p Thr157
Specificity
Detects human p27/Kip1 when phosphorylated at T157 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDKN1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1555 in the following application:

Publications
Read Publications using
AF1555 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p27/Kip1 [p Thr157] Antibody

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Background

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. The Akt protein kinase phosphorylates T157, an amino acid residue that lies within a nuclear localization signal for p27, to prevent nuclear localization and thus preclude the growth inhibitory function of p27.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p27/Kip1 Antibody (AF1555)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


Filter By Application
Immunoprecipitation
(1)
Western Blot
(11)
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Filter By Species
Human
(12)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF1555 Applications Species
YF Lian, YL Huang, YJ Zhang, DM Chen, JL Wang, H Wei, YH Bi, ZW Jiang, P Li, MS Chen, YH Huang CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation Theranostics, 2019;9(26):8392-8408. 2019 [PMID: 31754404] (Western Blot, Human) Western Blot Human
Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation. Oncogene, 2015;34(43):5447-59. 2015 [PMID: 25684140] (Immunoprecipitation, Human) Immunoprecipitation Human
Wang Y, Zhou D, Chen S SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1. Mol Endocrinol, 2014;28(6):935-48. 2014 [PMID: 24739041] (Western Blot, Human) Western Blot Human
Lin, Victor T, Lin, Vivian Y, Lai, Yun-Ju, Chen, Chen-Sha, Liu, Kang, Lin, Weei-Chi, Lin, Fang-Tsy TRIP6 regulates p27 KIP1 to promote tumorigenesis. Mol Cell Biol, 2013;33(7):1394-409. 2013 [PMID: 23339869] (Western Blot, Human) Western Blot Human
Shiozaki A, Shen-Tu G, Bai X, Iitaka D, De Falco V, Santoro M, Keshavjee S, Liu M XB130 mediates cancer cell proliferation and survival through multiple signaling events downstream of Akt. PLoS ONE, 2012;7(8):e43646. 2012 [PMID: 22928011] (Western Blot, Human) Western Blot Human
Wen S, So Y, Singh K Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer. Oncogene, 2012;31(14):1771-80. 2012 [PMID: 21841827] (Western Blot, Human) Western Blot Human
Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A Homeodomain-interacting Protein Kinase-2 Stabilizes p27kip1 by Its Phosphorylation at Serine 10 and Contributes to Cell Motility. J. Biol. Chem., 2011;286(33):29005-13. 2011 [PMID: 21715331] (Western Blot, Human) Western Blot Human
Santi SA, Lee H Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells. PLoS ONE, 2011;6(1):e14614. 2011 [PMID: 21297943] (Western Blot, Human) Western Blot Human
Yang W, Ju JH, Lee KM, Shin I Akt isoform-specific inhibition of MDA-MB-231 cell proliferation. Cell. Signal., 2011;23(1):19-26. 2011 [PMID: 20688159] (Western Blot, Human) Western Blot Human
Rodriguez-Ubreva FJ, Cariaga-Martinez AE, Cortes MA, Romero-De Pablos M, Ropero S, Lopez-Ruiz P, Colas B Knockdown of protein tyrosine phosphatase SHP-1 inhibits G1/S progression in prostate cancer cells through the regulation of components of the cell-cycle machinery. Oncogene, 2010;29(3):345-55. 2010 [PMID: 19838216] (Western Blot, Human) Western Blot Human
Show All 12 Publications.

Review for p27/Kip1 Antibody (AF1555) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1555:
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Western Blot p27/Kip1 AF1555
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reviewed by:
Anonymous
WB Human 01/11/2017
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Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell line SKBR3 and this product
SpeciesHuman
LotIVA061607A

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Anonymous
01/11/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDKN1B
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