p27/Kip1 [p Thr157] Antibody

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291‑G1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL Human Phospho-p27/Kip1 (T157) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p27/Kip1 (T157) was detected at approximately 27 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

p27/Kip1 [p Thr157] Antibody Summary

• Immunogen: Phosphopeptide containing human p27/Kip1 T157 site
• Modification: p Thr157
• Specificity: Detects human p27/Kip1 when phosphorylated at T157 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDKN1B
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p27/Kip1 [p Thr157] Antibody

• CDKN1B
• CDKN4
• cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• cyclin-dependent kinase inhibitor 1B
• Cyclin-dependent kinase inhibitor p27
• Kip1
• KIP1P27KIP1
• MEN1B
• MEN4
• p27
• p27Kip1

Background

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. The Akt protein kinase phosphorylates T157, an amino acid residue that lies within a nuclear localization signal for p27, to prevent nuclear localization and thus preclude the growth inhibitory function of p27.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p27/Kip1 Antibody (AF1555)(12)

Publications using AF1555

• YF Lian, YL Huang, YJ Zhang, DM Chen, JL Wang, H Wei, YH Bi, ZW Jiang, P Li, MS Chen, YH Huang CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation Theranostics, 2019-10-22;9(26):8392-8408. 2019-10-22 [PMID: 31754404] (Western Blot, Human)
• Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation. Oncogene, 2015-02-16;34(43):5447-59. 2015-02-16 [PMID: 25684140] (Immunoprecipitation, Western Blot, Human)
• Wang Y, Zhou D, Chen S SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1. Mol Endocrinol, 2014-04-16;28(6):935-48. 2014-04-16 [PMID: 24739041] (Western Blot, Human)
• Lin , Victor T, Lin , Vivian Y, Lai , Yun-Ju, Chen , Chen-Sha, Liu , Kang, Lin , Weei-Chi, Lin , Fang-Tsy TRIP6 regulates p27 KIP1 to promote tumorigenesis. Mol Cell Biol, 2013-01-22;33(7):1394-409. 2013-01-22 [PMID: 23339869] (Western Blot, Human)
• Shiozaki A, Shen-Tu G, Bai X, Iitaka D, De Falco V, Santoro M, Keshavjee S, Liu M XB130 mediates cancer cell proliferation and survival through multiple signaling events downstream of Akt. PLoS ONE, 2012-08-23;7(8):e43646. 2012-08-23 [PMID: 22928011] (Western Blot, Human)
• Wen S, So Y, Singh K Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer. Oncogene, 2011-08-15;31(14):1771-80. 2011-08-15 [PMID: 21841827] (Western Blot, Human)
• Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A Homeodomain-interacting Protein Kinase-2 Stabilizes p27kip1 by Its Phosphorylation at Serine 10 and Contributes to Cell Motility. J. Biol. Chem., 2011-06-29;286(33):29005-13. 2011-06-29 [PMID: 21715331] (Western Blot, Human)
• Santi SA, Lee H Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells. PLoS ONE, 2011-01-31;6(1):e14614. 2011-01-31 [PMID: 21297943] (Western Blot, Human)
• Yang W, Ju JH, Lee KM, Shin I Akt isoform-specific inhibition of MDA-MB-231 cell proliferation. Cell. Signal., 2010-08-02;23(1):19-26. 2010-08-02 [PMID: 20688159] (Western Blot, Human)
• Rodriguez-Ubreva FJ, Cariaga-Martinez AE, Cortes MA, Romero-De Pablos M, Ropero S, Lopez-Ruiz P, Colas B Knockdown of protein tyrosine phosphatase SHP-1 inhibits G1/S progression in prostate cancer cells through the regulation of components of the cell-cycle machinery. Oncogene, 2009-10-19;29(3):345-55. 2009-10-19 [PMID: 19838216] (Western Blot, Human)
• Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma. Clin. Cancer Res., 2009-01-01;15(1):81-90. 2009-01-01 [PMID: 19118035] (Western Blot, Human)
• Tseng PH, Wang YC, Weng SC, Weng JR, Chen CS, Brueggemeier RW, Shapiro CL, Chen CY, Dunn SE, Pollak M, Chen CS Overcoming trastuzumab resistance in HER2-overexpressing breast cancer cells by using a novel celecoxib-derived phosphoinositide-dependent kinase-1 inhibitor. Mol. Pharmacol., 2006-08-03;70(5):1534-41. 2006-08-03 [PMID: 16887935] (Western Blot, Human)

Review for p27/Kip1 Antibody (AF1555) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Elisa Lupino WB Human 01/11/2017

Summary

• Application: Western Blot
• Sample Tested: Human breast cancer cell line SKBR3 and this product
• Species: Human
• Lot: IVA061607A

Bioinformatics

• Gene Symbol: CDKN1B