Caspase 3 - a Reliable Marker for Index of Apoptosis Induction

Fri, 03/11/2016 - 15:16

Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. The latter are responsible for the cleavage of PARP (poly ADP-ribose polymerase), actin and SREBP, which are associated with apoptosis [1]. Because of its role in coordinating the destruction of cellular structures, Caspase 3 is often regarded as an executioner or effector caspase and considered as a marker in various immunoassays for apoptosis related experiments.

Caspase 3 antibody



caspase-3 antibody

Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] – WB analysis of lysates from 2 uM staurosporine treated HeLa cells showing the detection of both pro (full-length; at 32kDa) and active (cleaved; 10-20 kDa) forms of Caspase-3.


Caspase-3 (Pro and Active) Antibody [NB100-56112] - IHC analysis of Caspase-3 expression in formalin-fixed, paraffin-embedded human breast ductal carcinoma in situ using this antibody at 1:2000. Staining is seen in the the cancerous ducts, but not in the normal lobulus.

Novus Biologicals offers several different antibodies for Caspase 3 (pro and active) and majority of these antibodies are cited in research publications from journals of high repute.

Caspase-3 (Pro and Active) antibody [NB100-56112] was recently used for Western blot experiments in lysates of human colon xenograft tumors from mice subjected to treatments with Pterostilbene and 39-hydroxypterostilbene and the activation of caspase 3 was considered as apoptosis index [2]. Palavicini et al (2013) employed Novus Caspase-3 antibody clone 31A1067 (along with PCNA and DCX ) for IHC-frozen sections on brain tissues from neonatal Ran-/-, Ran+/- and littermate wild type P1 pups. They found similar number of caspase 3 +ve positive cells in RanBP9-/- and WT brains, and related RanBP to defective neuronal differentiation which results in subnormal brain development followed by neonatal lethality [3]. Kim et al. (2015) used Caspase-3 antibody (clone CPP32 4-1-18) for IHC analysis of formalin-fixed paraffin-embedded tissue sections from human cases of luminal A invasive breast ductal carcinoma. The staining was performed on autostainer with biotinylated secondary-streptavidin HRP - DAB based detection method and the findings established that the low expression of SIRT1 as well as caspase-3 predicts a higher risk of lymph node metastasis and disease-related recurrence [4].

Compiled By: Subhash Gangar


  1. McIlwain DR, Berger T, Mak TW.  Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol. 2013 Apr 1;5(4):a008656
  2. Cheng TC, Lai CS, Chung MC et al. Potent anti-cancer effect of 3'-hydroxypterostilbene in human colon xenograft tumors. PLoS One. 2014 Nov 12;9(11):e111814
  3. Palavicini JP, Lloyd BN, Hayes CD et al. RanBP9 Plays a Critical Role in Neonatal Brain Development in Mice. PLoS One. 2013 Jun 26;8(6):e66908
  4. Kim H, Lee KH, Park IA et al. Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer. Virchows Arch. 2015 Nov;467(5):563-70

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