Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. The latter are responsible for the cleavage of PARP (poly ADP-ribose polymerase), actin and SREBP, which are associated with apoptosis . Because of its role in coordinating the destruction of cellular structures, Caspase 3 is often regarded as an executioner or effector caspase and considered as a marker in various immunoassays for apoptosis related experiments.
Novus Biologicals offers several different antibodies for Caspase 3 (pro and active) and majority of these antibodies are cited in research publications from journals of high repute.
Caspase-3 (Pro and Active) antibody [NB100-56112] was recently used for Western blot experiments in lysates of human colon xenograft tumors from mice subjected to treatments with Pterostilbene and 39-hydroxypterostilbene and the activation of caspase 3 was considered as apoptosis index . Palavicini et al (2013) employed Novus Caspase-3 antibody clone 31A1067 (along with PCNA and DCX ) for IHC-frozen sections on brain tissues from neonatal Ran-/-, Ran+/- and littermate wild type P1 pups. They found similar number of caspase 3 +ve positive cells in RanBP9-/- and WT brains, and related RanBP to defective neuronal differentiation which results in subnormal brain development followed by neonatal lethality . Kim et al. (2015) used Caspase-3 antibody (clone CPP32 4-1-18) for IHC analysis of formalin-fixed paraffin-embedded tissue sections from human cases of luminal A invasive breast ductal carcinoma. The staining was performed on autostainer with biotinylated secondary-streptavidin HRP - DAB based detection method and the findings established that the low expression of SIRT1 as well as caspase-3 predicts a higher risk of lymph node metastasis and disease-related recurrence .
Compiled By: Subhash Gangar
- McIlwain DR, Berger T, Mak TW. Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol. 2013 Apr 1;5(4):a008656
- Cheng TC, Lai CS, Chung MC et al. Potent anti-cancer effect of 3'-hydroxypterostilbene in human colon xenograft tumors. PLoS One. 2014 Nov 12;9(11):e111814
- Palavicini JP, Lloyd BN, Hayes CD et al. RanBP9 Plays a Critical Role in Neonatal Brain Development in Mice. PLoS One. 2013 Jun 26;8(6):e66908
- Kim H, Lee KH, Park IA et al. Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer. Virchows Arch. 2015 Nov;467(5):563-70