PCNA Antibody (PC10) [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, ZeSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
PC10
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

PCNA Antibody (PC10) [FITC] Summary

Immunogen
Protein A-rat PCNA fusion obtained from pC2T
Marker
Proliferation Marker
Specificity
Specific for PCNA p36 protein expressed at high levels in proliferating cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PCNA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This PCNA (PC10) antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation, Immunocytochemistry/Immunofluorescence (PMID: 22068968) Flow Cytometry and Western blot. It detects a band at 30 kDa by Western blot. For IHC on paraffin sections heat-mediated citrate buffer antigen retrieval is recommended.ELISA was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Yeast (S. pombe and S. cerevisiae), Drosophila melanogaster, Zebrafish, Rat, Mouse, Monkey, Chicken, Rabbit and Fish.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PCNA Antibody (PC10) [FITC]

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • proliferating cell nuclear antigen

Background

PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PCNA Antibody (NB500-106F) (0)

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for PCNA Antibody (NB500-106F) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB500-106APC
DyLight 350 Labeled NB500-106UV
DyLight 405 Labeled NB500-106V
DyLight 488 Labeled NB500-106G
DyLight 550 Labeled NB500-106R
DyLight 650 Labeled NB500-106C
DyLight 680 Labeled NB500-106FR
DyLight 755 Labeled NB500-106IR
HRP Labeled NB500-106H
PE Labeled NB500-106PE
PerCP Labeled NB500-106PCP

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Bioinformatics Tool for PCNA Antibody (NB500-106F)

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Blogs on PCNA.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

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Bioinformatics

Gene Symbol PCNA