SSSCA1 Antibody (OTI2F5)

Images

 
Western Blot: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSSCA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSSCA1.
Immunohistochemistry-Paraffin: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody.
Flow Cytometry: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSSCA1 antibody, and then ...read more
Immunohistochemistry-Paraffin: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody.
Flow Cytometry: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Analysis of Hela cells, using anti-SSSCA1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Analysis of Jurkat cells, using anti-SSSCA1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-P
Clone
OTI2F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.48 mg/ml

Order Details

SSSCA1 Antibody (OTI2F5) Summary

Immunogen
Full length human recombinant protein of human SSSCA1 (NP_006387) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SSSCA1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry-Paraffin
Theoretical MW
21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
SSSCA1 Knockout 293T Cell Lysate

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.48 mg/ml
Purity
Affinity purified

Alternate Names for SSSCA1 Antibody (OTI2F5)

  • Autoantigen p27
  • centromeric autoantigen (27kD)
  • p27
  • Sjoegren syndrome/scleroderma autoantigen 1
  • Sjogren syndrome/scleroderma autoantigen 1
  • Sjogren's syndrome/scleroderma autoantigen 1

Background

This antigen is recognized by a subset of anti-centromere antibodies from patients with scleroderma and/or Sjogren's syndrome. Subcellular localization has not yet been established. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SSSCA1 Antibody (NBP2-03198) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-74359AF350
Alexa Fluor 405 NBP2-74359AF405
Alexa Fluor 488 NBP2-74359AF488
Alexa Fluor 532 NBP2-74359AF532
Alexa Fluor 594 NBP2-74359AF594
Alexa Fluor 647 NBP2-74359AF647
Alexa Fluor 700 NBP2-74359AF700
Alexa Fluor 750 NBP2-74359AF750
Allophycocyanin NBP2-74359APC
Allophycocyanin/Cy7 NBP2-74359APCCY7
Biotin NBP2-74359B
DyLight 350 NBP2-74359UV
DyLight 405 NBP2-74359V
DyLight 488 NBP2-74359G
DyLight 550 NBP2-74359R
DyLight 594 NBP2-74359DL594
DyLight 650 NBP2-74359C
DyLight 680 NBP2-74359FR
DyLight 755 NBP2-74359IR
FITC NBP2-74359F
HRP NBP2-74359H
Janelia Fluor 549 NBP2-74359JF549
Janelia Fluor 646 NBP2-74359JF646
PE NBP2-74359PE
PE/Atto594 NBP2-74359PEATT594
PE/Cy5.5 NBP2-74359PECY55
PE/Cy7 NBP2-74359PECY7
PerCP NBP2-74359PCP

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Bioinformatics

Gene Symbol SSSCA1
Entrez