PBR Antibody (CL13358) - BSA Free

Western Blot: PBR Antibody (CL13358) [NBP3-18567] - Analysis in human cell line PC-3 and human cell line CACO-2.

Immunocytochemistry/Immunofluorescence: PBR Antibody (CL13358) [NBP3-18567] - Staining of A-431 cells using the Anti-TSPO monoclonal antibody, showing specific staining in mitochondria and vesicles in green. Nuclear probes are is visualized in blue (where available).

Immunohistochemistry-Paraffin: PBR Antibody (CL13358) [NBP3-18567] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: PBR Antibody (CL13358) [NBP3-18567] - Analysis in human skin and liver tissues using NBP3-18567 antibody. Corresponding TSPO RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: PBR Antibody (CL13358) [NBP3-18567] - Staining of human skin shows strong granular cytoplasmic positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: PBR Antibody (CL13358) [NBP3-18567] - Staining of human tonsil shows strong granular positivity in lymphoid cells.

Immunohistochemistry-Paraffin: PBR Antibody (CL13358) [NBP3-18567] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in astrocytes.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL13358
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

PBR Antibody (CL13358) - BSA Free Summary

• Description: Novus Biologicals Mouse PBR Antibody (CL13358) - BSA Free (NBP3-18567) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from P30536, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSPO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for PBR Antibody (CL13358) - BSA Free

• benzodiazepine peripheral binding site
• BZRPDBI
• IBP
• MBRPBS
• mDRC
• Mitochondrial benzodiazepine receptor
• PBR
• PBRBPBS
• PBRS
• peripheral benzodiazepine receptor
• Peripheral-type benzodiazepine receptor
• pk18
• PKBSbenzodiazapine receptor (peripheral)
• PTBR
• translocator protein (18kDa)
• translocator protein
• TSPO

Background

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSPO