PBR Antibodies
We offer PBR Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Sandwich ELISA, Western Blot. Each PBR Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PBR Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PBR Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PBR polyclonal antibodies and browse our PBR monoclonal antibody catalog.
Alternate Names for PBR Antibodies
anti-PBR antibody, anti-TSPO antibody, anti-benzodiazepine peripheral binding site antibody, anti-BZRPDBI antibody, anti-IBP antibody, anti-MBRPBS antibody, anti-mDRC antibody, anti-Mitochondrial benzodiazepine receptor antibody, anti-PBRBPBS antibody, anti-PBRS antibody, anti-peripheral benzodiazepine receptor antibody, anti-Peripheral-type benzodiazepine receptor antibody, anti-pk18 antibody, anti-PKBSbenzodiazapine receptor (peripheral) antibody, anti-PTBR antibody, anti-translocator protein (18kDa) antibody, anti-translocator protein antibody
11 Results for "pbr" in Primary Antibodies
![Western Blot: PBR Antibody [NB100-41398] - Staining of A431 cell lysate.(35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PBR-Antibody-Western-Blot-NB100-41398-img0011.jpg "Western Blot: PBR Antibody [NB100-41398] - Staining of A431 cell lysate.(35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PBR Antibody [NB100-41398] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/PBR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41398-img0010.jpg)
![Immunohistochemistry: PBR Antibody [NB100-41398] - Correlative TSPO immunohistochemistry in a primary human grade III astrocytoma xenotransplant, Tumor + White Matter Tract (40X). Image collected and cropped by CiteAb from the following publication (www.journals.plos.org/plosone/article?id=10.1371/journal.pone.0141659) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PBR-Antibody-Immunohistochemistry-NB100-41398-img0013.jpg)
![Flow Cytometry: PBR Antibody [NB100-41398] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PBR-Antibody-Flow-Cytometry-NB100-41398-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PBR Antibody [NB100-41398] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative cLontrol: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PBR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41398-img0009.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PBR-Antibody-Immunohistochemistry-Paraffin-NB100-41398-img0005.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PBR-Antibody-Immunohistochemistry-Paraffin-NB100-41398-img0007.jpg)
![Immunohistochemistry: PBR Antibody [NB100-41398] - Human tumor microarrays (TMAs). Pilocytic astrocytoma TSPO immunohistochemistry (A) and Glioblastoma multiforme TSPO immunohistochemistry (B). Image collected and cropped by CiteAb from the following publication (www.journals.plos.org/plosone/article?id=10.1371/journal.pone.0141659) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PBR-Antibody-Immunohistochemistry-NB100-41398-img0012.jpg)
![Immunohistochemistry: PBR Antibody [NB100-41398] - [18F]PBR06 selectivity in primary human grade IV glioblastoma xenotransplant.(A) Dynamic PET image (axial) pre-infusion of cold analog, with [18F]PBR06 uptake primarily confined to the tumor. (B) Dynamic PET image (axial) post-infusion, showing nearly total displacement of [18F]PBR06. (C) Time-activity curves of injected [18F]PBR06 in tumor (green) & contralateral brain (blue). (D) Correlative TSPO immunohistochemistry, Tumor + White Matter Tract (40X). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41398_goat-polyclonal-pbr-antibody-31020241534322.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Human tumor microarrays (TMAs).Pilocytic astrocytoma TSPO immunohistochemistry (A) & TSPO immunohistochemical scoring (C). Glioblastoma multiforme TSPO immunohistochemistry (B) & TSPO immunohistochemical scoring (D). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41398_goat-polyclonal-pbr-antibody-310202415304277.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Human tumor microarrays (TMAs).Pilocytic astrocytoma TSPO immunohistochemistry (A) & TSPO immunohistochemical scoring (C). Glioblastoma multiforme TSPO immunohistochemistry (B) & TSPO immunohistochemical scoring (D). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41398_goat-polyclonal-pbr-antibody-310202415392564.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Primary human grade IV glioblastoma xenotransplant.(A) Dynamic PET image (coronal) of advanced tumor in the right hemisphere of the brain with [18F]PBR06 uptake confined primarily to the tumor. Arrows indicate tumor & infiltration into left hemisphere. (B) Time-activity curves of injected [18F]PBR06 in tumor (green) & contralateral brain (blue) [30]. (C) Correlative TSPO immunohistochemistry, Tumor + White Matter Tract (40X). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41398_goat-polyclonal-pbr-antibody-310202415371958.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Primary human grade III astrocytoma xenotransplant.(A) T2-weighted MRI (coronal) visualizes advanced tumor in the right hemisphere of the brain. (B) Correlative dynamic PET image (coronal) of same advanced tumor in the right hemisphere of the brain, with [18F]PBR06 uptake primarily confined to the tumor & co-localizing with tumor tissue visualized by MRI. Top arrows indicate tumor & infiltration into left hemisphere. (C) Fused MRI (A) PET (B) image. (D) Time-activity curves of injected [18F]PBR06 in tumor (green) & contralateral brain (blue). Correlative vimentin immunohistochemistry: (E) Gross; (F) Tumor + White Matter Tract (40X); (G) Tumor (40X). (H) Correlative dynamic PET image, axial view along yellow line in (B); arrows indicate tumor & infiltration into left hemisphere. Correlative TSPO immunohistochemistry: (I) Gross; (J) Tumor + White Matter Tract (40X); (K) Tumor (40X). (L) Dynamic PET image (axial) of control cohort. Correlative CD68 immunohistochemistry: (M) Gross; (N) Tumor + White Matter Tract (40X); (O) Tumor (40X). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41398_goat-polyclonal-pbr-antibody-310202415535136.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Primary human grade III astrocytoma xenotransplant.(A) T2-weighted MRI (coronal) visualizes advanced tumor in the right hemisphere of the brain. (B) Correlative dynamic PET image (coronal) of same advanced tumor in the right hemisphere of the brain, with [18F]PBR06 uptake primarily confined to the tumor & co-localizing with tumor tissue visualized by MRI. Top arrows indicate tumor & infiltration into left hemisphere. (C) Fused MRI (A) PET (B) image. (D) Time-activity curves of injected [18F]PBR06 in tumor (green) & contralateral brain (blue). Correlative vimentin immunohistochemistry: (E) Gross; (F) Tumor + White Matter Tract (40X); (G) Tumor (40X). (H) Correlative dynamic PET image, axial view along yellow line in (B); arrows indicate tumor & infiltration into left hemisphere. Correlative TSPO immunohistochemistry: (I) Gross; (J) Tumor + White Matter Tract (40X); (K) Tumor (40X). (L) Dynamic PET image (axial) of control cohort. Correlative CD68 immunohistochemistry: (M) Gross; (N) Tumor + White Matter Tract (40X); (O) Tumor (40X). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41398_goat-polyclonal-pbr-antibody-31020241612624.jpg)
![Immunohistochemistry: PBR Antibody [NB100-41398] - Primary human grade III astrocytoma xenotransplant.(A) T2-weighted MRI (coronal) visualizes advanced tumor in the right hemisphere of the brain. (B) Correlative dynamic PET image (coronal) of same advanced tumor in the right hemisphere of the brain, with [18F]PBR06 uptake primarily confined to the tumor & co-localizing with tumor tissue visualized by MRI. Top arrows indicate tumor & infiltration into left hemisphere. (C) Fused MRI (A) PET (B) image. (D) Time-activity curves of injected [18F]PBR06 in tumor (green) & contralateral brain (blue). Correlative vimentin immunohistochemistry: (E) Gross; (F) Tumor + White Matter Tract (40X); (G) Tumor (40X). (H) Correlative dynamic PET image, axial view along yellow line in (B); arrows indicate tumor & infiltration into left hemisphere. Correlative TSPO immunohistochemistry: (I) Gross; (J) Tumor + White Matter Tract (40X); (K) Tumor (40X). (L) Dynamic PET image (axial) of control cohort. Correlative CD68 immunohistochemistry: (M) Gross; (N) Tumor + White Matter Tract (40X); (O) Tumor (40X). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41398_goat-polyclonal-pbr-antibody-310202415533823.jpg)
![Western Blot: PBR Antibody [NBP1-45769] - Analysis of PBRexpression using:Lane 1: Jurkat cell lysateLane 2: Recombinant PBRLane 3: Rat kidney tissue lysate](http://images.novusbio.com/fullsize/PBR-Antibody-Western-Blot-NBP1-45769-img0003.jpg "Western Blot: PBR Antibody [NBP1-45769] - Analysis of PBRexpression using:Lane 1: Jurkat cell lysateLane 2: Recombinant PBRLane 3: Rat kidney tissue lysate")
![Western Blot: PBR Antibody (4I9K6) [NBP3-16497] - Western blot analysis of extracts of various cell lines, using PBR Rabbit mAb (NBP3-16497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PBR-Antibody-4I9K6-Western-Blot-NBP3-16497-img0004.jpg "Western Blot: PBR Antibody (4I9K6) [NBP3-16497] - Western blot analysis of extracts of various cell lines, using PBR Rabbit mAb (NBP3-16497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: PBR Antibody (4I9K6) [NBP3-16497] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202511383738.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [NBP3-16497] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-522025126538.jpg)
![Immunocytochemistry/ Immunofluorescence: PBR Antibody (4I9K6) [NBP3-16497] - Confocal imaging of U-2 OS cells using PBR Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-5220251242571.jpg)
![Immunocytochemistry/ Immunofluorescence: PBR Antibody (4I9K6) [NBP3-16497] - Confocal imaging of NIH/3T3 cells using PBR Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202511402780.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [NBP3-16497] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-5220251265341.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [NBP3-16497] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202511402736.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [NBP3-16497] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-5220251242581.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [NBP3-16497] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202511361246.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [PBR] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202517202950.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [PBR] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202517152719.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [PBR] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-5220251726064.jpg)
![Immunocytochemistry/ Immunofluorescence: PBR Antibody (4I9K6) [PBR] - Confocal imaging of U-2 OS cells using PBR Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202517172844.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [PBR] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202517202995.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [PBR] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202517202927.jpg)
![Immunohistochemistry: PBR Antibody (4I9K6) [PBR] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using PBR Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202517184561.jpg)
![Immunocytochemistry/ Immunofluorescence: PBR Antibody (4I9K6) [PBR] - Confocal imaging of NIH/3T3 cells using PBR Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16497_rabbit-pbr-mab-4i9k6-52202517202978.jpg)
![Immunoprecipitation: PBR Antibody (23G2) [NBP3-26270] - Immunoprecipitating PBR in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26270 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26270 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26270_rabbit-pbr-mab-23g2-26220241322152.jpg "Immunoprecipitation: PBR Antibody (23G2) [NBP3-26270] - Immunoprecipitating PBR in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26270 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26270 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: PBR Antibody (23G2) [NBP3-26270] - Image of PBR Antibody (23G2) diluted at 1:117 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26270_rabbit-pbr-mab-23g2-27220248471913.jpg)
![Immunohistochemistry: PBR Antibody (23G2) [NBP3-26270] - Image of PBR Antibody (23G2) diluted at 1:117 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26270_rabbit-pbr-mab-23g2-2722024820394.jpg)
![Western Blot: PBR Antibody (23G2) [NBP3-26270] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue. All lanes: PBR Antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 19, 11 KDa Observed band size: 19 KDa](http://images.novusbio.com/fullsize/nbp3-26270_rabbit-pbr-mab-23g2-282202411442621.jpg)
![Immunocytochemistry/Immunofluorescence: PBR Antibody (23G2) [NBP3-26270] - Staining of PC3 cells with PBR Antibody (23G2) at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26270_rabbit-pbr-mab-23g2-28220241312226.jpg)
![Flow Cytometry: PBR Antibody (23G2) [NBP3-26270+156:156D172158:192153:192D172158:192149:192D172158:192145:192D1168:192] - Overlay histogram showing HepG2 cells stained with PBR Antibody (23G2) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26270_rabbit-pbr-mab-23g2-282202414312782.jpg)
![Western Blot: PBR Antibody (CL13358) [NBP3-18567] - Analysis in human cell line PC-3 and human cell line CACO-2.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13358-Western-Blot-NBP3-18567-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PBR Antibody (CL13358) [NBP3-18567] - Analysis in human cell line PC-3 and human cell line CACO-2.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PBR Antibody (CL13358) [NBP3-18567] - Staining of A-431 cells using the Anti-TSPO monoclonal antibody, showing specific staining in mitochondria and vesicles in green. Nuclear probes are is visualized in blue (where available).](http://images.novusbio.com/fullsize/PBR-Antibody-CL13358-Immunocytochemistry-Immunofluorescence-NBP3-18567-img0001.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody (CL13358) [NBP3-18567] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13358-Immunohistochemistry-Paraffin-NBP3-18567-img0006.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody (CL13358) [NBP3-18567] - Analysis in human skin and liver tissues using NBP3-18567 antibody. Corresponding TSPO RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13358-Immunohistochemistry-Paraffin-NBP3-18567-img0002.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody (CL13358) [NBP3-18567] - Staining of human skin shows strong granular cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13358-Immunohistochemistry-Paraffin-NBP3-18567-img0003.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody (CL13358) [NBP3-18567] - Staining of human tonsil shows strong granular positivity in lymphoid cells.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13358-Immunohistochemistry-Paraffin-NBP3-18567-img0004.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody (CL13358) [NBP3-18567] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in astrocytes.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13358-Immunohistochemistry-Paraffin-NBP3-18567-img0005.jpg)
![Western Blot: PBR Antibody (CL13359) [NBP3-18560] - Analysis in human cell line PC-3 and human cell line CACO-2.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13359-Western-Blot-NBP3-18560-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PBR Antibody (CL13359) [NBP3-18560] - Analysis in human cell line PC-3 and human cell line CACO-2.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PBR Antibody (CL13359) [NBP3-18560] - Staining of A-431 cells using the Anti-TSPO monoclonal antibody, showing specific staining in mitochondria and vesicles in green. Nuclear probes are is visualized in blue (where available).](http://images.novusbio.com/fullsize/PBR-Antibody-CL13359-Immunocytochemistry-Immunofluorescence-NBP3-18560-img0001.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody (CL13359) [NBP3-18560] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13359-Immunohistochemistry-Paraffin-NBP3-18560-img0006.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody (CL13359) [NBP3-18560] - Analysis in human skin and liver tissues using NBP3-18560 antibody. Corresponding TSPO RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13359-Immunohistochemistry-Paraffin-NBP3-18560-img0002.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody (CL13359) [NBP3-18560] - Staining of human skin shows strong granular cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13359-Immunohistochemistry-Paraffin-NBP3-18560-img0003.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody (CL13359) [NBP3-18560] - Staining of human tonsil shows moderate to strong granular positivity in lymphoid cells.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13359-Immunohistochemistry-Paraffin-NBP3-18560-img0004.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody (CL13359) [NBP3-18560] - Staining of human cerebral cortex shows moderate granular positivity in glial cells.](http://images.novusbio.com/fullsize/PBR-Antibody-CL13359-Immunohistochemistry-Paraffin-NBP3-18560-img0005.jpg)
![Western Blot: PBR Antibody [NBP1-51934] - Staining of Mouse Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence](http://images.novusbio.com/fullsize/PBR-Antibody-Western-Blot-NBP1-51934-img0005.jpg "Western Blot: PBR Antibody [NBP1-51934] - Staining of Mouse Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence")
![Immunohistochemistry-Paraffin: PBR Antibody [NBP1-51934] - Negative Control showing staining of paraffin embedded Mouse Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/PBR-Antibody-Immunohistochemistry-Paraffin-NBP1-51934-img0003.jpg)
![Flow Cytometry: PBR Antibody [NBP1-51934] - Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PBR-Antibody-Flow-Cytometry-NBP1-51934-img0004.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody [NBP1-51934] - Staining of paraffin embedded Mouse Kidney. Antibody at 10 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining (1:500).](http://images.novusbio.com/fullsize/PBR-Antibody-Immunohistochemistry-Paraffin-NBP1-51934-img0002.jpg)
![Western Blot: PBR Antibody [NBP3-17663] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/PBR-Antibody-Western-Blot-NBP3-17663-img0002.jpg "Western Blot: PBR Antibody [NBP3-17663] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Immunocytochemistry/Immunofluorescence: PBR Antibody [NBP3-17663] - Staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/PBR-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17663-img0001.jpg)
![ELISA: PBR Antibody (3D8-B2) [H00000706-M01] - Detection limit for recombinant GST tagged BZRP is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PBR-Antibody-3D8-B2-ELISA-H00000706-M01-img0001.jpg "ELISA: PBR Antibody (3D8-B2) [H00000706-M01] - Detection limit for recombinant GST tagged BZRP is approximately 10ng/ml as a capture antibody.")