Human RBP4 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human RBP4 Quantikine ELISA Kit Summary

• Background: The Quantikine Human RBP4 immunoassay is a 2.5 hour solid phase ELISA designed to measure human RBP4 in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human RBP4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RBP4 showed linear curves that were parallel to the standard curves obtained ...using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human RBP4.
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• Specificity: Natural and recombinant human RBP4
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: RBP4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human RBP4 Quantikine ELISA Kit

• interstitial
• Plasma retinol-binding protein
• RBP4
• retinol binding protein 4, plasma
• RetinolBinding Protein 4
• retinol-binding protein 4, plasma

Background

Retinol-binding protein 4 (RBP4) is a Lipocalin superfamily molecule that transports vitamin A (retinol) and retinaldehyde in the serum. Retinaldehyde is the critical chromophore in the rhodopsin photoreceptor, while both it and retinoic acid regulate a multitude of cellular differentiation and proliferation effects through the intracellular receptors RAR and RXR. RBP4 is secreted primarily by hepatocytes and adipocytes into the blood where the RBP4-retinol complex interacts with transthyretin (TTR). Formation of the complex with TTR increases the serum half-life of RBP4 by preventing RBP4 filtration through the kidney. The C-terminally processed forms of RBP4, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. RBP4 promotes hyperglycemia, and its upregulation in visceral and liver adipocytes leads to elevated serum levels.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for RBP4/Retinol-Binding Protein 4 (DRB400)(31)

Publications using DRB400

• Yadav, AS;Rubinow, KB;Zelter, A;Authement, AK;Kestenbaum, BR;Amory, JK;Isoherranen, N; Development and Validation of a Novel LC-MS/MS Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR) bioRxiv : the preprint server for biology 2025-03-04 [PMID: 40093066] (Human)
• Penkert, RR;Jones, BG;Tang, L;Su, Y;Jeha, S;Yang, J;Yang, W;Ferrolino, J;Strength, R;Pui, CH;Cross, SJ;Hurwitz, JL;Wolf, J; Association of Vitamin A and D Deficiencies with Infectious Outcomes in Children Undergoing Intensive Induction Therapy for Acute Lymphoblastic Leukemia The Journal of pediatrics 2024-06-14 [PMID: 38880379] (Human)
• Supriya, R;Shishvan, SR;Kefayati, M;Abednatanzi, H;Razi, O;Bagheri, R;Escobar, KA;Pashaei, Z;Saeidi, A;Shahrbanian, S;Bagchi, S;Sengupta, P;Al Kiyumi, MH;Heinrich, KM;Zouhal, H; Astaxanthin Supplementation Augments the Benefits of CrossFit Workouts on Semaphorin 3C and Other Adipokines in Males with Obesity Nutrients 2023-11-16 [PMID: 38004197] (Human)
• H L?rincz, B Ratku, S Csiha, I Seres, Z Szabó, G Paragh, M Harangi, S Somodi Impaired Organokine Regulation in Non-Diabetic Obese Subjects: Halfway to the Cardiometabolic Danger Zone International Journal of Molecular Sciences, 2023-02-18;24(4):. 2023-02-18 [PMID: 36835525] (Human)
• H Jeong, AT Armstrong, N Isoherrane, L Czuba, A Yang, K Zumpf, J Ciolino, E Torres, CS Stika, KL Wisner Temporal changes in the systemic concentrations of retinoids in pregnant and postpartum women PLoS ONE, 2023-02-16;18(2):e0280424. 2023-02-16 [PMID: 36795769] (Human)
• ME Rossholt, K Wendel, M Bratlie, MF Aas, G Gunnarsdot, D Fugelseth, AH Pripp, M Domellöf, K Størdal, T Stiris, SJ Moltu Vitamin A Status in Preterm Infants Is Associated with Inflammation and Dexamethasone Exposure Nutrients, 2023-01-14;15(2):. 2023-01-14 [PMID: 36678312] (Human)
• N Patel, RR Penkert, RE Sealy, SL Surman, BG Jones, K Ringwald-S, AC Ross, JL Hurwitz Retinol Binding Protein, Sunlight Hours, and the Influenza Virus-Specific Immune Response Biomedicines, 2022-09-19;10(9):. 2022-09-19 [PMID: 36140423] (Human)
• RR Penkert, M Azul, RE Sealy, BG Jones, J Dowdy, RT Hayden, L Tang, AC Ross, JS Hankins, JL Hurwitz Hypothesis: Low Vitamin A and D Levels Worsen Clinical Outcomes When Children with Sickle Cell Disease Encounter Parvovirus B19 Nutrients, 2022-08-19;14(16):. 2022-08-19 [PMID: 36014920] (Human)
• N Hirata, M Kiuchi, K Pak, R Fukuda, N Mochimaru, M Mitsui, K Yoshida Association between Maternal Characteristics and Immune Factors TGF-beta1, TGF-beta2, and IgA in Colostrum: An Exploratory Study in Japan Nutrients, 2022-08-09;14(16):. 2022-08-09 [PMID: 36014760] (Human)
• MM Hasan, MA Gazi, S Das, SM Fahim, F Hossaini, AR Khan, J Ferdous, MA Alam, M Mahfuz, T Ahmed Gut biomolecules (I-FABP, TFF3 and lipocalin-2) are associated with linear growth and biomarkers of environmental enteric dysfunction (EED) in Bangladeshi children Scientific Reports, 2022-08-16;12(1):13905. 2022-08-16 [PMID: 35974137] (Human)
• LC Czuba, EE Fay, J LaFrance, CK Smith, S Shum, SL Moreni, J Mao, N Isoherrane, MF Hebert Plasma Retinoid Concentrations Are Altered in Pregnant Women Nutrients, 2022-03-25;14(7):. 2022-03-25 [PMID: 35405978] (Human)
• IL Salazar, AST Lourenço, B Manadas, I Baldeiras, C Ferreira, AC Teixeira, VM Mendes, AM Novo, R Machado, S Batista, MDC Macário, M Grãos, L Sousa, MJ Saraiva, AACC Pais, CB Duarte Posttranslational modifications of proteins are key features in the identification of CSF biomarkers of multiple sclerosis Journal of Neuroinflammation, 2022-02-08;19(1):44. 2022-02-08 [PMID: 35135578] (Human)
• J Liu, C Nie, L Xue, Y Yan, S Liu, J Sun, M Fan, H Qian, H Ying, L Wang, Y Li Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4 Theranostics, 2021-07-13;11(17):8283-8300. 2021-07-13 [PMID: 34373742] (Human)
• Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males. Antioxidants (Basel), 2021-05-28;10(6):. 2021-05-28 [PMID: 34071378] (Human)
• D Tsakogiann, E Kalogera, F Zagouri, E Zografos, D Balalis, G Bletsa Determination of FABP4, RBP4 and the MMP-9/NGAL complex in the serum of women with breast cancer Oncology Letters, 2020-12-04;21(2):85. 2020-12-04 [PMID: 33376518] (Human)
• X Wang, Y Huang, J Gao, H Sun, M Jayachandr, S Qu Changes of serum retinol-binding protein 4 associated with improved insulin resistance after laparoscopic sleeve gastrectomy in Chinese obese patients Diabetol Metab Syndr, 2020-01-14;12(0):7. 2020-01-14 [PMID: 31956345] (Human)
• H Wessel, A Saeed, J Heegsma, MA Connelly, KN Faber, RPF Dullaart Plasma Levels of Retinol Binding Protein 4 Relate to Large VLDL and Small LDL Particles in Subjects with and without Type 2 Diabetes J Clin Med, 2019-10-25;8(11):. 2019-10-25 [PMID: 31717719] (Human)
• D Kostic, GPNS Beozzo, SB do Couto, AHT Kato, L Lima, P Palmeira, VLJ Krebs, V Bunduki, RPV Francisco, M Zugaib, FT Dénes, WB de Carvalh, VHK Koch The role of renal biomarkers to predict the need of surgery in congenital urinary tract obstruction in infants J Pediatr Urol, 2019-03-16;0(0):. 2019-03-16 [PMID: 30979613] (Human)
• JY Li, XX Chen, XH Lu, CB Zhang, QP Shi, L Feng Elevated RBP4 plasma levels were associated with diabetic retinopathy in type 2 diabetes Biosci. Rep., 2018-09-12;0(0):. 2018-09-12 [PMID: 30135138] (Human)
• YY Zhu, JL Zhang, L Liu, Y Han, X Ge, S Zhao Evaluation of serum retinol-binding protein-4 levels as a biomarker of poor short-term prognosis in ischemic stroke Biosci. Rep., 2018-09-12;0(0):. 2018-09-12 [PMID: 30038059] (Human)
• M Du, E Phelps, MJ Balangue, A Dockins, G Moiseyev, Y Shin, S Kane, L Otalora, JX Ma, R Farjo, KM Farjo Transgenic Mice Over-Expressing RBP4 Have RBP4-Dependent and Light-Independent Retinal Degeneration Invest. Ophthalmol. Vis. Sci., 2017-08-01;58(10):4095-4103. 2017-08-01 [PMID: 28813718] (Mouse)
• G Annibalini, F Lucertini, D Agostini, L Vallorani, A Gioacchini, E Barbieri, M Guescini, L Casadei, A Passalia, M Del Sal, G Piccoli, M Andreani, A Federici, V Stocchi Concurrent Aerobic and Resistance Training Has Anti-Inflammatory Effects and Increases Both Plasma and Leukocyte Levels of IGF-1 in Late Middle-Aged Type 2 Diabetic Patients Oxid Med Cell Longev, 2017-06-21;2017(0):3937842. 2017-06-21 [PMID: 28713486] (Human)
• S Karunanith, L Levi, J DeVecchio, G Karagkouni, O Reizes, JD Lathia, MF Kalady, N Noy RBP4-STRA6 Pathway Drives Cancer Stem Cell Maintenance and Mediates High-Fat Diet-Induced Colon Carcinogenesis Stem Cell Reports, 2017-07-06;0(0):. 2017-07-06 [PMID: 28689994] (Human)
• JL Hurwitz, BG Jones, RR Penkert, S Gansebom, Y Sun, L Tang, AM Bramley, S Jain, JA McCullers, SR Arnold Low Retinol-Binding Protein and Vitamin D Levels Are Associated with Severe Outcomes in Children Hospitalized with Lower Respiratory Tract Infection and Respiratory Syncytial Virus or Human Metapneumovirus Detection J. Pediatr., 2017-05-31;0(0):. 2017-05-31 [PMID: 28578159] (Human)
• M Du, A Martin, F Hays, J Johnson, RA Farjo, KM Farjo Serum retinol-binding protein-induced endothelial inflammation is mediated through the activation of toll-like receptor 4 Mol. Vis., 2017-03-31;23(0):185-197. 2017-03-31 [PMID: 28400700] (Mouse)
• Talsma E, Verhoef H, Brouwer I, Mburu-de Wagt A, Hulshof P, Melse-Boonstra A Proxy markers of serum retinol concentration, used alone and in combination, to assess population vitamin A status in Kenyan children: a cross-sectional study. BMC Med, 2015-02-11;13(0):30. 2015-02-11 [PMID: 25856672] (Human)
• Solini A, Stea F, Santini E, Bruno R, Duranti E, Taddei S, Ghiadoni L Adipocytokine levels mark endothelial function in normotensive individuals. Cardiovasc Diabetol, 2012-08-31;11(0):103. 2012-08-31 [PMID: 22938533] (Human)
• Ahn KJ, Choi EA, Kim J, Lee JH, Lee KH, Bang D, Cho SB Increased retinol-binding protein (RBP) 4 and anti-RBP4 antibody in alopecia areata. Br. J. Dermatol., 2011-10-01;165(4):837-44. 2011-10-01 [PMID: 21711327] (Human)
• Nakajima H, Nakajima K, Tarutani M, Morishige R, Sano S Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients. Arch. Dermatol. Res., 2011-06-17;303(6):451-5. 2011-06-17 [PMID: 21681565] (Human)
• Chan DC, Watts GF, Gan S Nonalcoholic fatty liver disease as the transducer of hepatic oversecretion of very-low-density lipoprotein-apolipoprotein B-100 in obesity. Arterioscler. Thromb. Vasc. Biol., 2010-02-11;30(5):1043-50. 2010-02-11 [PMID: 20150556] (Human)
• Solini A, Santini E, Madec S, Rossi C, Muscelli E Retinol-binding protein-4 in women with untreated essential hypertension. Am. J. Hypertens., 2009-06-25;22(9):1001-6. 2009-06-25 [PMID: 19556974] (Human)

FAQs for RBP4/Retinol-Binding Protein 4 (DRB400). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: RBP4