Apelin Antibody

Immunohistochemistry-Paraffin: Apelin Antibody [NBP2-31176] - Staining of human isolated adipocytes. Antibody dilution 1:2000.

• Reactivity: Hu, Mu, Po, Sh
• Applications: ELISA, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Apelin Antibody Summary

• Immunogen: Synthetic peptide derived from C terminal region of human Apelin
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APLN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31767185). Sheep reactivity reported in scientific literature (PMID: 31412301). Use in Porcine reported in secitific publication PMID: 32456363

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris 0,1M, glycine 0,1M, sucrose 2%
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Lyophilized powder. Reconstitute in 100ul distilled water.

Alternate Names for Apelin Antibody

• AGTRL1 ligand
• AGTRL1 Lligand
• APEL
• Apelin
• apelin, AGTRL1 ligand
• APJ endogenous ligand
• APLN
• XNPEP2

Background

Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ, a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues, with highest levels reported for heart and lungs, as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ; the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates; more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis, by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described, including endothelium-dependent vasodilatation, vasoconstriction through direct action on smooth muscle and positive inotropism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apelin Antibody (NBP2-31176)(3)

Publications using NBP2-31176

• Dall'Aglio C, Mercati F, De Felice E et al. Influence of Different Feed Physical Forms on Mandibular Gland in Growing Pigs Animals (Basel) 2020-05-24 [PMID: 32456363] (IF/IHC, Porcine)
• Troisi A, Dall'Aglio C, Maranesi M et al. Presence and expression of apelin and apelin receptor in bitch placenta Theriogenology 2019-11-18 [PMID: 31767185] (IF/IHC, Mouse)
• Mercati F, Scocco P, Maranesi M et al. Apelin system detection in the reproductive apparatus of ewes grazing on semi-natural pasture Theriogenology 2019-08-07 [PMID: 31412301] (IF/IHC, Sheep)

Bioinformatics

• Gene Symbol: APLN