MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')
2Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')
2Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rat Anti-Human/Mouse PBEF/ Visfatin Monoclonal Antibody (Catalog # MAB4044, filled histogram) or isotype control antibody (Catalog #
MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')
2Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />