PBEF/Visfatin/NAMPT Antibody (BLR058F)

Western Blot: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human and mouse NAMPT/PBEF/Visfatin by WB of RKO, MCF-7, K-562, GaMG, HEK293T, HeLa, U2OS, LNCaP, Hep-G2, Jurkat, and NIH 3T3 lysate. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-GAPDH.

Immunocytochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE NCI-H460 by ICC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Flow Cytometry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Rabbit antiNAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunoprecipitation: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368), rabbit anti-NAMPT/PBEF/Visfatin antibodies NB100-594, and one other rabbit anti-NAMPT/PBEF/Visfatin antibody.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: BLR058F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PBEF/Visfatin/NAMPT Antibody (BLR058F) Summary

• Description: Novus Biologicals Rabbit PBEF/Visfatin/NAMPT Antibody (BLR058F) (NBP2-76368) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Immunogen sequence between amino acids 400 and 450
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NAMPT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl
  • Immunocytochemistry/ Immunofluorescence 1:100 to 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 to 1:500
  • Immunoprecipitation 5 ul/1 mg lysate
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Phosphate Buffered Saline (PBS), BSA-Free
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for PBEF/Visfatin/NAMPT Antibody (BLR058F)

• EC 2.4.2.12
• MGC117256
• NAmPRTase
• NAMPT
• nicotinamide phosphoribosyltransferase
• NMPRTase
• PBEF
• PBEF1
• PBEF1110035O14Rik
• PBEF1DKFZp666B131
• Pre-B cell-enhancing factor
• pre-B-cell colony enhancing factor 1
• Pre-B-cell colony-enhancing factor 1
• VF
• Visfatin

Background

PBEF is also known as visfatin and was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Intracellularly, PBEF acts as a cytosolic enzyme involved in nicotinamide adenine dinucleotide (NAD) synthesis. PBEF levels positively correlate with obesity and type 2 diabetes mellitus and can bind the insulin receptor to lower blood sugar.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAMPT