Pax2 Antibody (CL9469) - BSA Free

Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Analysis in human kidney and prostate tissues using NBP2-88934 antibody. Corresponding PAX2 RNA-seq data are presented for the same tissues.

Western Blot: Pax2 Antibody (CL9469) [NBP2-88934] - Analysis in human cell line EFO-21.

Immunocytochemistry/Immunofluorescence: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of EFO-21 cells using the Anti-PAX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human endometrium shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human kidney shows strong nuclear positivity in cells in distal tubules.

Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human prostate shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human renal cancer shows moderate nuclear positivity in tumor cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL9469
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Pax2 Antibody (CL9469) - BSA Free Summary

• Description: Novus Biologicals Mouse Pax2 Antibody (CL9469) - BSA Free (NBP2-88934) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from Q02962, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Pax2 Antibody (CL9469) - BSA Free

• paired box 2
• paired box gene 2
• paired box homeotic gene 2
• paired box protein Pax-2
• Pax2

Background

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAX2