EN1/Engrailed 1 Antibody (3H2)

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04] - Analysis of monoclonal antibody to EN1 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml

ELISA: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04] - Detection limit for recombinant GST tagged EN1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC, IHC-P, IP
• Clone: 3H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EN1/Engrailed 1 Antibody (3H2) Summary

• Immunogen: EN1 (NP_001417, 266 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SQQPLVWPAWVYCTRYSDRPSSGPRTRKLKKKKNEKEDKRPRTAFTAEQLQRLKAEFQANRYITEQRRQTLAQELSLNESQIKIWFQNKRAKIKKATGIKNGLALHLMAQGLYNHSTTTVQDKDESE*
• Specificity: EN1 (3H2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EN1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for IHC-P.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EN1/Engrailed 1 Antibody (3H2)

• engrailed homeobox 1
• engrailed homolog 1
• Homeobox protein en-1
• homeobox protein engrailed-1
• hu-En-1

Background

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EN1/Engrailed 1 Antibody (H00002019-M04)(1)

Publication using H00002019-M04

• Veenvliet JV, Dos Santos MT, Kouwenhoven WM et al. Specification of dopaminergic subsets involves interplay of En1 and Pitx3. Development. 2013-07-17 [PMID: 23863478]

Bioinformatics

• Gene Symbol: EN1
• Entrez:
  • 2019(Human)
• OMIM:
  • 131290(Human)
• Uniprot:
  • NP_001417(Human)