LIM1 Antibody (OTI2D5)

Images

 
Western Blot: LIM1 Antibody (2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.
Immunohistochemistry-Paraffin: LIM1 Antibody (2D5) [NBP2-01926] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.
Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - Analysis of Jurkat cells, using anti-LHX1 antibody (Red) compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: LIM1 Antibody (2D5) [NBP2-01926] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LIM1 Antibody (2D5) [NBP2-01926] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LIM1 Antibody (2D5) [NBP2-01926] - Staining of paraffin-embedded Human Kidney tissue using anti-LIM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LIM1 Antibody (2D5) [NBP2-01926] - Staining of paraffin-embedded Human liver tissue using anti-LIM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LIM1 Antibody (2D5) [NBP2-01926] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.
Flow Cytometry: LIM1 Antibody (2D5) [NBP2-01926] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIM1 antibody, and then analyzed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI2D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

LIM1 Antibody (OTI2D5) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 100-362 of human LHX1(NP_005559) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LHX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
44.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-01926 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LIM1 Antibody (OTI2D5)

  • hLim-1
  • Homeobox protein Lim-1
  • LHX1
  • LIM homeobox 1
  • LIM homeobox protein 1
  • LIM1
  • LIM-1LIM/homeobox protein Lhx1
  • LIM1MGC138141
  • MGC126723

Background

This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIM1 Antibody (NBP2-01926)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LIM1 Antibody (NBP2-01926) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71134AF350
Alexa Fluor 405 NBP2-71134AF405
Alexa Fluor 488 NBP2-71134AF488
Alexa Fluor 532 NBP2-71134AF532
Alexa Fluor 594 NBP2-71134AF594
Alexa Fluor 647 NBP2-71134AF647
Alexa Fluor 700 NBP2-71134AF700
Alexa Fluor 750 NBP2-71134AF750
Allophycocyanin NBP2-71134APC
Allophycocyanin/Cy7 NBP2-71134APCCY7
Biotin NBP2-71134B
DyLight 350 NBP2-71134UV
DyLight 405 NBP2-71134V
DyLight 488 NBP2-71134G
DyLight 550 NBP2-71134R
DyLight 594 NBP2-71134DL594
DyLight 650 NBP2-71134C
DyLight 680 NBP2-71134FR
DyLight 755 NBP2-71134IR
FITC NBP2-71134F
HRP NBP2-71134H
Janelia Fluor 549 NBP2-71134JF549
Janelia Fluor 646 NBP2-71134JF646
PE NBP2-71134PE
PE/Atto594 NBP2-71134PEATT594
PE/Cy5.5 NBP2-71134PECY55
PE/Cy7 NBP2-71134PECY7
PerCP NBP2-71134PCP

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Bioinformatics Tool for LIM1 Antibody (NBP2-01926)

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Blogs on LIM1

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Calculators

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Bioinformatics

Gene Symbol LHX1
Entrez