Wnt-1 Antibody (10C8)

Western Blot: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.

Immunocytochemistry/Immunofluorescence: Wnt-1 Antibody (10C8) [NBP1-51575] - Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis of human LAdrenal tissues using WNT1 mouse mAb.

Flow Cytometry: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis of Hela cells using WNT1 mouse mAb (green) and negative control (purple).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
• Clone: 10C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Wnt-1 Antibody (10C8) Summary

• Immunogen: Purified recombinant fragment of WNT1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: WNT1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Wnt-1 Antibody (10C8)

• Int-1
• INT1Proto-oncogene Int-1 homolog
• proto-oncogene Wnt-1
• wingless-type MMTV integration site family, member 1 (oncogene INT1)
• wingless-type MMTV integration site family, member 1
• Wnt1
• Wnt-1

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-1 Antibody (NBP1-51575)(1)

Publication using NBP1-51575

• Ardiana D, Herwanto N, Prakoeswa CR, Agusni I Expression of WNT1 signalling proteins after phototherapy exposure in vitiligo Journal of Pakistan Association of Dermatologists 2019-01-01 (IF/IHC, Human)

Bioinformatics

• Gene Symbol: WNT1