PAX8 Antibody

Images

 
Western Blot: PAX8 Antibody [NBP1-32440] - 293T whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PAX8 Antibody [NBP1-32440] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs). Schematic description of the two-stage protocol applied to iPSC-renal ...read more
Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Pax8 immunoreactivity in canine renal carcinoma. Primary antibody incubated with tissue for 1h at room temperature. IHC-P image submitted by a verified ...read more
Chromatin Immunoprecipitation: PAX8 Antibody [NBP1-32440] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of PAX8 antibody The precipitated DNA was detected by PCR with primer set ...read more
Immunocytochemistry/ Immunofluorescence: PAX8 Antibody [NBP1-32440] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Rat thyroid gland. PAX8 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - DLD1 xenograft . PAX8 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Mouse liver. PAX8 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: PAX8 Antibody [NBP1-32440] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of PAX8 ...read more
Simple Western: PAX8 Antibody [NBP1-32440] - Simple Western lane view shows a specific band for PAX8 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, XpSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PAX8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PAX8. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Xenopus (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAX8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Immunoprecipitation 1:500 - 1:1000
  • Simple Western 1:10
  • Single Cell Western 50 ug/mL
  • Western Blot 1:5000-1:20000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
SCW is validated using 786-O cells.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-32440 in the following applications:

Publications
Read Publications using
NBP1-32440 in the following applications:

Reactivity Notes

Xenopus laevis (91%). Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for PAX8 Antibody

  • paired box 8
  • paired box gene 8
  • paired box protein Pax-8
  • paired domain gene 8

Background

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PAX8 Antibody (NBP1-32440)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: CHIP-SEQ, ChIP, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
CHIP-SEQ
(1)
ChIP
(2)
ICC/IF
(4)
IHC
(1)
IHC-P
(2)
IP
(1)
WB
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-32440 Applications Species
Zhou M, Zhou S, Han K et al. Th1 immune maturation effects of Nocardia rubra cell-wall skeleton via PI3K/Akt/PAX8 regulatory axis Science progress Apr 27 2022 [PMID: 35473474] (CHIP-SEQ, Mouse) CHIP-SEQ Mouse
Yoon HJ, Chung YS, Lee YJ Et al. Cancer Patient Tissueoid with Self-Homing Nano-Targeting of Metabolic Inhibitor Advanced science (Weinheim, Baden-Wurttemberg, Germany) Oct 18 2021 [PMID: 34664430] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Malacrida B, Nichols S, Maniati E et al. A human multi-cellular model shows how platelets drive production of diseased extracellular matrix and tissue invasion iScience Jun 25 2021 [PMID: 34189439]
Delaine-Smith R, Maniati E, Malacrida B et al. Modelling TGF beta R and Hh pathway regulation of prognostic matrisome molecules in ovarian cancer iScience May 1 2021 [PMID: 34189438] (ICC/IF, Human) ICC/IF Human
Wang J, Li N, Huang ZB et al. HBx regulates transcription factor PAX8 stabilization to promote the progression of hepatocellular carcinoma Oncogene Aug 7 2019 [PMID: 31391550] (WB, Human) WB Human
di Martino S, De Luca G, Grassi L et al. Renal cancer: new models and approach for personalizing therapy. J. Exp. Clin. Cancer Res. Sep 5 2018 [PMID: 30185225] (IHC-P, Human) IHC-P Human
Adler EK, Corona RI, Lee JM et al. The PAX8 cistrome in epithelial ovarian cancer. Oncotarget 2017 Nov 27 [PMID: 29312534] (ChIP) ChIP
Kang HS, Kumar D, Liao G et al. GLIS3 is indispensable for TSH/TSHR-dependent thyroid hormone biosynthesis and follicular cell proliferation J. Clin. Invest. 2017 Oct 30 [PMID: 29083325] (IHC-P, Mouse) IHC-P Mouse
Ludwig K, Alaggio R, Zin A et al. BCOR-CCNB3 Undifferentiated Sarcoma-Does Immunohistochemistry Help in the Identification. Pediatr. Dev. Pathol. Jan 1 2017 [PMID: 28420319]
Elias KM, Emori MM, Westerling T et al. Epigenetic remodeling regulates transcriptional changes between ovarian cancer and benign precursors. JCI insight. Aug 18 2016 [PMID: 27617304] (IP, ChIP, ICC/IF, Human) IP, ChIP, ICC/IF Human
Show All 11 Publications.

Review for PAX8 Antibody (NBP1-32440) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Canine.

Reviews using NBP1-32440:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
All Applications
Filter by Species
Canine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin PAX8 NBP1-32440
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4
reviewed by:
Kevin Thorburn
Immunohistochemistry-Paraffin Canine 10/29/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedkidney
SpeciesCanine
Lot42613

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PAX8 Antibody (NBP1-32440) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Kevin Thorburn
10/29/2021
Application: Immunohistochemistry-Paraffin
Species: Canine

Bioinformatics

Gene Symbol PAX8