pan Cadherin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody [NB200-592] - A computer-generated micrograph showing the superimposed immunofluorescent staining of chicken lens cells using FITC-phalloidin (green), ...read more
Immunohistochemistry-Paraffin: N-Cadherin Antibody [NB200-592] - Mouse cervical cancer tissue stained with N-Cadherin antibody (green). Nuclei were counterstained with DAPI (Blue). Antigen retrieval was done with pepsin ...read more
Immunohistochemistry: N-Cadherin Antibody [NB200-592] - Section of human heart.

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, Av, Bv, Ca, Ch, Fi, Rb, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

pan Cadherin Antibody Summary

Immunogen
Synthetic peptide: DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH by a Glutaraldehyde linker. Sequence corrresponds to the C-terminal amino acids of N-Cadherin (Chicken), with an extra N-terminal lysine residue (24 amino acids).
Localization
Cell membrane
Marker
Mesenchymal Cells Marker
Specificity
Recognizes all cadherin members. Rabbit Anti-Pan Cadherin shows specific reactivity with a distinct 135 kDa band on chicken or rabbit heart extract blots using indirect immunoblotting.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
This antibody is useful for: Western Blot. IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: On chicken and rabbit heart extract this antibody detects a single band at 135 kD.
Positive Control
E-Cadherin Lysate (NBL1-09024)
Reviewed Applications
Read 1 Review rated 5
using
NB200-592 in the following applications:

Publications
Read Publications using
NB200-592 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for pan Cadherin Antibody

  • Arc-1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDHE
  • cell-CAM 120/80
  • ECAD
  • E-cadherin
  • Epithelial cadherin
  • LCAM
  • UVOE-Cadherin
  • uvomorulin

Background

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca++-dependent cell-cell adhesion.1,2,3 The N-terminal part of these molecules is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually, a cytoplasmic C-terminal tail4 which mediates interaction with the microfilament system through molecules such as catenins, plakoglobin, vinculin, and a-actinin. Cadherins which are primarily located in areas of cell-cell contacts, are involved in selective cell sorting and in the mechanical cytoplasmic response. They are implicated in morphogenetic processes, intercellular signalling and tumor invasiveness and metastasis. Multiple cadherins were characterized from diverse species and tissues including E-Cadherin, N-Cadherin (A-CAM), P-Cadherin, V-Cadherin, R-Cadherin and T-Cadherin. Specific antibodies against a highly conserved sequence from the cytoplasmic C-terminal of N-Cadherin have been prepared.5,6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for pan Cadherin Antibody (NB200-592)(5)

Review for pan Cadherin Antibody (NB200-592) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-P.

Reviews using NB200-592:
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IHC-P
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin pan Cadherin NB200-592
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reviewed by:
Sanghyuk Chung
IHC-P Mouse 12/20/2016
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse cervix
SpeciesMouse

Comments

Commentsantigen retrieval with pepsin treatment

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Sanghyuk Chung
12/20/2016
Application: IHC-P
Species: Mouse