PABPC4 Antibody - BSA Free

Immunohistochemistry-Paraffin: PABPC4 Antibody [NBP2-19671] - Paraffin-embedded human lung carcinoma. PABPC4 antibody dilution: 1:500.

Immunohistochemistry-Paraffin: PABPC4 Antibody [NBP2-19671] - Paraffin-embedded mouse lung. PABPC4 antibody dilution: 1:500.

Immunohistochemistry: PABPC4 Antibody [NBP2-19671] - PABPC4 antibody detects Pabpc4 protein at myotomes on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. PABPC4 antibody (NBP2-19671) dilution: 1:200.

Western Blot: PABPC4 Antibody [NBP2-19671] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABPC4 antibody (NBP2-19671) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Ze, Rt, Bv, Xp, Ze
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PABPC4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PABPC4 Antibody - BSA Free (NBP2-19671) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PABPC4. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rat (96%), Xenopus (91%), Zebrafish (90%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PABPC4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PABPC4 Antibody - BSA Free

• APP1FLJ43938
• APP-1PABP-4
• Inducible poly(A)-binding protein
• IPABP
• iPABPpoly(A)-binding protein, cytoplasmic 4 (inducible form)
• PABP4Activated-platelet protein 1
• poly(A) binding protein, cytoplasmic 4 (inducible form)
• Poly(A)-binding protein 4
• polyadenylate-binding protein 4

Background

Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PABPC4