Human Endothelin-1 QuantiGlo ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Human Endothelin-1 QuantiGlo ELISA Kit Summary

Background
The QuantiGlo Human Endothelin-1 Chemiluminescent Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Endothelin-1 levels in cell culture supernates, serum, EDTA plasma, and urine without extraction. It contains synthetic human Endothelin-1 and antibodies raised against the synthetic factor. This immunoassay has been shown to accurately quantitate human Endothelin-1. Result...s obtained using natural human Endothelin-1 showed dose curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values of natural human Endothelin-1.
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Specificity
Natural and synthetic human Endothelin-1
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
EDN1

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using QET00B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Endothelin-1 QuantiGlo ELISA Kit

  • ARCND3
  • EDN1
  • endothelin 1
  • Endothelin-1
  • ET1
  • HDLCQ7
  • PPET1
  • preproendothelin-1
  • QME

Background

Endothelin-1 (ET-1), a peptide of 21 amino acid (aa) residues, is a pleiotropic molecule best known for its action as a potent vasoconstrictor (1). Originally isolated from porcine aortic endothelial cells, ET-1 is one of a family of three proteins encoded by distinct genes that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3) (2, 3). ET-2 and ET-3 differ from ET-1 by 2 and 6 amino acids, respectively (1, 2). All members of the Endothelin family contain two essential disulfide bridges and six conserved aa residues at the C-terminus. Human ET-1 is initially synthesized as a pre-pro-polypeptide of 212 amino acids (2, 4). It is proteolytically cleaved by a signal peptidase to produce pro-ET-1 and further processed by a Furin-like protease to yield a 38 aa peptide termed Big ET-1 (5, 6). Big ET-1 is then cleaved by the membrane-bound metalloprotease Endothelin-converting enzyme (ECE-1), producing the potent 21 aa mature form ET-1 (aa 1-21) (7, 8). Alternatively, ET-1 may exist in an active 31 aa form (ET-1 (aa 1-31)) following cleavage of Big ET-1 by chymase (9-12). The vascular endothelium is an abundant source of ET-1 (3, 13). It may also be expressed by leukocytes, smooth muscle cells, mesangial cells, cardiac myocytes, and astrocytes (14, 15). ET-1 can be induced in endothelial cells by many factors including mechanical stimulation, various hormones, and pro-inflammatory cytokines (16). Production is inhibited by nitric oxide (NO), Prostacyclin, and atrial natriuretic peptide (ANP) (17-19). 
Two receptors for the Endothelin family have been cloned and designated ETA and ETB (20-23). ETA and ETB belong to the large family of heptahelical G protein-coupled receptors. The ETA receptor shows a higher affinity for ET-1 than for ET-2 and lowest affinity for ET-3, while the ETB receptor shows approximately equal affinity for each of the three Endothelins (21, 22, 24). ETA is primarily responsible for the vasoconstrictor effects of ET-1 and is expressed by blood vessel smooth muscle cells (25, 26). The ETB receptor is also present in smooth muscle and the endothelia of blood vessels, kidney, lung, and brain (27). ET-1 has the ability to activate an array of signaling cascades including classical phosphatidylinositol turnover pathways leading to downstream PKC activation and Ca2+ mobilization (28-32). Other potential signaling mediators activated or produced by ET-1 include PI 3-kinase/Akt, NO, FAK, and Rho GTPases (32-37). ET-1 signaling may also be mediated indirectly via transactivation of the EGF receptor leading to downstream signaling by Ras and MAP kinases (38, 39). Injection of a single dose of ET-1 produces an initial decrease in systemic blood pressure followed by a prolonged increase in blood pressure (16, 40, 41). Blockade of Endothelin receptors with a systemic injection of an ETA/ETB antagonist causes progressive vasodilation, and elevated levels of ET-1 are found in some forms of human hypertension (42, 43). ET-1 also stimulates cardiac contraction and the growth of cardiac myocytes, regulates the release of vasoactive substances, and stimulates smooth muscle cell mitogenesis (32, 44-46). It also acts as a pro-survival factor for endothelial cells and regulates secretion by hypothalamic and pituitary cells (47, 48). ET-1 may control inflammatory responses by promoting the adhesion and migration of neutrophils and stimulating the production of pro-inflammatory cytokines (49-53). It has also been implicated in cancer progression at several levels including regulating the proliferation and migration of tumor cells and acting as a pro-angiogenic factor (54, 55). In addition, ET-1 has putative roles in other pathologies including septic shock, atherosclerosis, heart failure, renal insufficiency, pulmonary hypertension, and cerebrovascular conditions associated with subarachnoid hemorrhage (15, 56-63).

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Publications for Endothelin-1 (QET00B)(40)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Equine, Ovine, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Rabbit.


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Human
(27)
Mouse
(1)
Rat
(5)
Equine
(1)
Ovine
(1)
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(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using QET00B Applications Species
M Haffke, H Freitag, G Rudolf, M Seifert, W Doehner, N Scherbakov, L Hanitsch, K Wittke, S Bauer, F Konietschk, F Paul, J Bellmann-S, C Kedor, C Scheibenbo, F Sotzny Endothelial dysfunction and altered endothelial biomarkers in patients with post-COVID-19 syndrome and chronic fatigue syndrome (ME/CFS) Journal of Translational Medicine, 2022;20(1):138. 2022 [PMID: 35317812] (Human) Human
AD Kaze, X Gao, SK Musani, A Bidulescu, AG Bertoni, M Abdalla, JB Echouffo-T Association of Plasma Endothelin-1 with Blood Pressure Progression Among African Americans: The Jackson Heart Study American heart journal, 2022;0(0):. 2022 [PMID: 34986393] (Human) Human
N Guo, P Wang, J Yang, X Yang, M van der Vo, M Wildwater, J Wei, X Tang, M Wang, H Yang Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals Biomarkers Relevant to Personalized Treatments Frontiers in Pharmacology, 2021;12(0):664320. 2021 [PMID: 34194326] (Human) Human
M Žarak, A Perovi?, M Njire Brat, S Šupraha Go, J Dumi? Adaptive response triggered by the repeated SCUBA diving is reflected in cardiovascular, muscular, and immune biomarkers Physiological Reports, 2021;9(2):e14691. 2021 [PMID: 33463896] (Human) Human
LG Douma, K Solocinski, SH Masten, DH Barral, SJ Barilovits, LA Jeffers, KD Alder, R Patel, CS Wingo, KD Brown, BD Cain, ML Gumz EDN1-AS, A Novel Long Non-coding RNA Regulating Endothelin-1 in Human Proximal Tubule Cells Front Physiol, 2020;11(0):209. 2020 [PMID: 32231591] (Human) Human
X Yang, W Yang, DG McVey, G Zhao, J Hu, RN Poston, M Ren, K Willeit, S Coassin, J Willeit, TR Webb, NJ Samani, M Mayr, S Kiechl, S Ye FURIN Expression in Vascular Endothelial Cells Is Modulated by a Coronary Artery Disease-Associated Genetic Variant and Influences Monocyte Transendothelial Migration J Am Heart Assoc, 2020;9(4):e014333. 2020 [PMID: 32067586] (Human) Human
Y Chen, D Wu, L Sun Clinical Significance of High-Mobility Group Box 1 Protein (HMGB1) and Nod-Like Receptor Protein 3 (NLRP3) in Patients with Ulcerative Colitis Med. Sci. Monit., 2020;26(0):e919530. 2020 [PMID: 31901930] (Human) Human
F Mayyas, N Saadeh, K Al-Muqbel, DR Van Wagone Plasma endothelin-1 levels are increased in atrial fibrillation patients with hyperthyroidism PLoS ONE, 2018;13(12):e0208206. 2018 [PMID: 30513109] (Human) Human
M Chen, Z Li, Z Zhang, Y Du, Y Zhang, M Xu, C Sun Intervention of integrative medicine treatment has impact on serum levels of ET-1, TNF-?, MLT in RA-CVD Saudi J Biol Sci, 2018;25(5):959-964. 2018 [PMID: 30108447] (Human) Human
KL Clines, GA Clines DKK1 and Kremen Expression Predicts the Osteoblastic Response to Bone Metastasis Transl Oncol, 2018;11(4):873-882. 2018 [PMID: 29772510] (Human) Human
Show All 40 Publications.

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Bioinformatics

Gene Symbol EDN1