ATF2 [p Thr69] Antibody

Images

 
Western Blot: ATF2 [p Thr69] Antibody [NB100-81798] - Analysis of extracts from 3T3 cells untreated or treated with Anisomycin using ATF2 (Phospho-Thr69 or 51) antibody
Immunocytochemistry/ Immunofluorescence: ATF2 [p Thr69] Antibody [NB100-81798] - Staining of methanol-fixed Hela cells using ATF2 (Phospho-Thr69 or 51) antibody
Immunohistochemistry: ATF2 [p Thr69] Antibody [NB100-81798] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF-2 (phospho-Thr69 or 51) antibody NB100-81798.
Western Blot: ATF2 [p Thr69] Antibody [NB100-81798] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submitted by a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

ATF2 [p Thr69] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ATF2 around the phosphorylation site of threonine 69 or 51 (D-Q-TP-P-T).
Modification
p Thr69
Specificity
Detects endogenous levels of ATF2 only when phosphorylated at threonine 69 or 51.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Western Blot 1:500
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-81798 in the following applications:

Publications
Read Publication using
NB100-81798 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATF2 [p Thr69] Antibody

  • activating transcription factor 2formerly
  • ATF2
  • cAMP responsive element binding protein 2
  • cAMP-responsive element-binding protein 2
  • CREB2
  • CREBP1
  • CRE-BP1
  • cyclic AMP-dependent transcription factor ATF-2
  • HB16
  • MGC111558
  • mXBP
  • TREB7

Background

ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATF2 Antibody (NB100-81798)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Review for ATF2 Antibody (NB100-81798) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-81798:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot ATF2 NB100-81798
Enlarge
4
reviewed by:
Pauline Jeannot
WB Human 10/17/2021
View

Summary

ApplicationWestern Blot
Sample TestedU251 glioma cell line; whole cell lysate
SpeciesHuman
Lot5117

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Recent Reviews

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2
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1
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Pauline Jeannot
10/17/2021
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATF2
Entrez
Uniprot