TTP Antibody (OTI8B5)

Western Blot: TTP Antibody (8B5) [NBP2-02105] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTP.

Immunocytochemistry/Immunofluorescence: TTP Antibody (OTI8B5) [NBP2-02105] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TTP.

Immunohistochemistry-Paraffin: TTP Antibody (8B5) [NBP2-02105] - Staining of paraffin-embedded Human prostate tissue using anti-TTP mouse monoclonal antibody.

Flow Cytometry: TTP Antibody (8B5) [NBP2-02105] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TTP antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: TTP Antibody (8B5) [NBP2-02105] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TTP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TTP Antibody (8B5) [NBP2-02105] - Staining of paraffin-embedded Human colon tissue using anti-TTP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TTP Antibody (8B5) [NBP2-02105] - Staining of paraffin-embedded Human endometrium tissue using anti-TTP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TTP Antibody (8B5) [NBP2-02105] - Staining of paraffin-embedded Human Ovary tissue using anti-TTP mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI8B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TTP Antibody (OTI8B5) Summary

• Description: Novus Biologicals Mouse TTP Antibody (OTI8B5) (NBP2-02105) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ZFP36 (NP_003398) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZFP36
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 33.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TTP Antibody (OTI8B5)

• EZNFTranscription factor 17
• HKL1Zinc finger protein eZNF
• KID-1
• KID1TCF-17
• TCF17
• zinc finger protein 354A

Background

Probable regulatory protein with a novel zinc finger structure involved in regulating the response to growth factors. Has been experimentally shown to be able to bind zinc

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZFP36
• Entrez:
  • 7538(Human)