TOB1 Antibody

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Human TOB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5228) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TOB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TOB1 Antibody Summary

• Immunogen: E. coli-derived recombinant human TOB1
  Met1-Asn345
  Accession # P50616
• Specificity: Detects endogenous human TOB1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TOB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TOB1 Antibody

• APRO6
• MGC104792
• PIG49
• protein Tob1
• TOB1
• TOBMGC34446
• Transducer of erbB-2 1
• transducer of ERBB2, 1
• TROB
• TROB1

Background

TOB1 (transducer of ErbB2 protein 1) is a 45 kDa member of the BTG family of anti-proliferative proteins. It is ubiquitously expressed, and serves to negatively regulate cell cycle progression from G 0G 1 to S phase. This is accomplished by TOB1 suppression of cyclin D1 expression, which is abrogated by Erk2-mediated phosphorylation of Ser152/154/164 in response to growth factor stimulation. Human TOB1 is 345 amino acids (aa) in length and contains one NES (aa 2-14), a bipartite NLS (aa 18-40), a BTG domain (aa 42-130) that possesses antiproliferative activity, and two PAM domains (aa 133-143 and 268-278) that bind inducible PABP and regulate gene transcription. Human and mouse TOB1 (aa 1-345) are 94% aa identical when fourteen mouse-specific glutamines are excluded from the alignment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOB1 Antibody (AF5228)(1)

Publication using AF5228

• Wen H, Wang M, Gong S et al. Human Umbilical Cord Mesenchymal Stem Cells Attenuate Abdominal Aortic Aneurysm Progression in Sprague Dawley Rats: Implication of Vascular Smooth Muscle Cell Phenotypic Modulation Stem Cells Dev. 2020-06-03 [PMID: 32486904]

Bioinformatics

• Gene Symbol: TOB1
• Uniprot:
  • P50616()