PABPC4 Antibody

Western Blot: PABPC4 Antibody [NB100-74594] - Detection of Human and Mouse PABP4 on HeLa whole cell lysate using NB100-74594. PABP4 was also immunoprecipitated by rabbit anti-PABP4 antibody NB100-74593.

Immunohistochemistry-Paraffin: PABPC4 Antibody [NB100-74594] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- PABP4 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PABPC4 Antibody Summary

• Description: Novus Biologicals Rabbit PABPC4 Antibody (NB100-74594) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-PABPC4 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 594 and 644 of human Poly(A)-binding protein 4 using the numbering given in entry NP_003810.1 (GeneID 8761).
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PABPC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PABPC4 Antibody

• APP1FLJ43938
• APP-1PABP-4
• Inducible poly(A)-binding protein
• IPABP
• iPABPpoly(A)-binding protein, cytoplasmic 4 (inducible form)
• PABP4Activated-platelet protein 1
• poly(A) binding protein, cytoplasmic 4 (inducible form)
• Poly(A)-binding protein 4
• polyadenylate-binding protein 4

Background

Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PABPC4 Antibody (NB100-74594)(3)

Publications using NB100-74594

• Xiang K, Bartel Dp The molecular basis of coupling between poly(A)-tail length and translational efficiency eLife 2021-07-02 [PMID: 34213414]
• Datu AK, Bag J. Enhanced Translation of mRNAs Encoding Proteins Involved in mRNA Translation during Recovery from Heat Shock. PLoS One 2013-05-16 [PMID: 23696868] (WB, Human)
• Bhattacharjee RB, Bag J. Depletion of Nuclear Poly(A) Binding Protein PABPN1 Produces a Compensatory Response by Cytoplasmic PABP4 and PABP5 in Cultured Human Cells. PLoS One. 2012-12-31 [PMID: 23300856] (IP, Human)

Bioinformatics

• Gene Symbol: PABPC4
• Entrez:
  • 8761(Human)
  • 230721(Mouse)
• Uniprot:
  • Q13310(Human)