PABPC4 Antibody

Images

 
Independent Antibodies: Western Blot: PABPC4 Antibody [NB100-74593] - Detection of PABP4 on HeLa whole cell lysate (5, 15, and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ...read more
Immunohistochemistry-Paraffin: PABPC4 Antibody [NB100-74593] - Section of human prostate carcinoma (left) and colon carcinoma (right). Affinity purified rabbit anti- PABP4 used at 1:200 (1 ug/mL). Detection by DAB.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP, IHC-Fr (-), Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PABPC4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 525 and 575 of human Poly(A)-binding protein 4 using the numbering given in entry NP_003810.1 (GeneID 8761).
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PABPC4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mL Lysate
  • Single Cell Western 10 ug/mL
  • Western Blot 1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in Single Cell Western reported in scientific literature (PMID: 31601799).
Publications
Read Publications using
NB100-74593 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PABPC4 Antibody

  • APP1FLJ43938
  • APP-1PABP-4
  • Inducible poly(A)-binding protein
  • IPABP
  • iPABPpoly(A)-binding protein, cytoplasmic 4 (inducible form)
  • PABP4Activated-platelet protein 1
  • poly(A) binding protein, cytoplasmic 4 (inducible form)
  • Poly(A)-binding protein 4
  • polyadenylate-binding protein 4

Background

Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PABPC4 Antibody (NB100-74593)(4)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PABPC4
Entrez
Uniprot