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P4HA1 Antibody

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Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody [NB100-57852] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852] - Staining of paraffin embedded Human Spleen with antibody at 7 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Bv, CaSpecies Glossary
Applications ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

P4HA1 Antibody Summary

Immunogen
This P4HA1 Antibody was developed against a peptide with sequence C-DFARKDEPDAFKE corresponding to internal region according to NP_001017962.1.
Epitope
DFARKDEPDAFKE
Specificity
This antibody is expected to recognize both reported isoforms (NP_000908.2 and NP_001017962.1).
Predicted Species
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
P4HA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 7 ug/mL
  • Immunohistochemistry-Paraffin 7 ug/mL
  • Peptide ELISA Detection limit 1:128000
Application Notes
IHC-P usage reported in scientific literature (PMID: 25587023).
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
293 Whole Cell Lysate
P4HA1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-57852 in the following applications:

Publications
Read Publications using
NB100-57852 in the following applications:

  • 1 publication
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24993042).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for P4HA1 Antibody

  • 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
  • 2-oxoglutarate-4-dioxygenase subunit alpha-1
  • 4-PH Alpha-1
  • alpha polypeptide I
  • EC 1.14.11.2
  • P4HA
  • P4HA1
  • Procollagen-proline
  • prolyl 4-hydroxylase, alpha polypeptide I

Background

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-57852
Species: Hu, Mu, Bv, Ca
Applications: ELISA, ICC/IF, IHC

Publications for P4HA1 Antibody (NB100-57852)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(5)
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(5)
Mouse
(1)
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Showing Publications 1 - 9 of 9.
Publications using NB100-57852 Applications Species
Eriksson J, Le Joncour V, Jahkola T et al. Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls Mol Oncol 2020-04-01 [PMID: 32053263] (WB, Human) WB Human
Islam A, Fossum V, Hansen AK et al. In vitro chondrogenic potency of surplus chondrocytes from autologous transplantation procedures does not predict short-term clinical outcomes. BMC Musculoskelet Disord 2019-01-10 [PMID: 30630436] (WB, Human) WB Human
Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014-01-01 [PMID: 25587023] (IHC-P, Human) IHC-P Human
Cowling RT, Yeo SJ, Kim IJ et al. Discoidin domain receptor 2 germline gene deletion leads to altered heart structure and function in the mouse Am J Physiol Heart Circ Physiol. 2014-07-02 [PMID: 24993042] (WB, Mouse) WB Mouse
Xiang L, Gilkes DM, Chaturvedi P et al. Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer. J Mol Med (Berl). 2013-11-20 [PMID: 24248265]
Gilkes DM, Chaturvedi P, Bajpai S et al. Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res 2013-06-01 [PMID: 23539444] (WB, Human) WB Human
Gilkes DM, Bajpai S, Chaturvedi P et al. HIF-1 Promotes Extracellular Matrix Remodeling under Hypoxic Conditions by Inducing P4HA1, P4HA2, and PLOD2 Expression in Fibroblasts. J Biol Chem 2013-02-19 [PMID: 23423382] (WB, Human) WB Human
Van Duyn Graham L, Sweetwyne MT, Pallero MA, Murphy-Ullrich JE. Intracellular calreticulin regulates multiple steps in fibrillar collagen expression, trafficking, and processing into the extracellular matrix. J Biol Chem 2010-03-05 [PMID: 20044481]
Annunen P, Helaakoski T, Myllyharju J et al. Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer. J Biol Chem 1997-07-11 [PMID: 9211872]

Review for P4HA1 Antibody (NB100-57852) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-57852:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Human 08/06/2010
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Summary

ApplicationWestern Blot
Sample TestedMDa-231, Sample Amount: 50ug
SpeciesHuman

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Verified Customer
08/06/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol P4HA1
Entrez
Uniprot