P4HA1 Antibody Summary
Peptide with sequence C-DFARKDEPDAFKE corresponding to internal region according to NP_001017962.1.
This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Western Blot 0.1 - 0.1 ug/ml Immunohistochemistry-Paraffin 5 ug/ml Peptide ELISA Detection limit 1:32000
WB: Approx. 60 kDa band observed in lysates of HEK293, A431 and NIH3T3 (calculated MW of 61.0 kDa band according to NP_000908.2 and NP_001017962.1). An additional band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P usage is reported in scientific literature (PMID: 25587023).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications using NB100-57852 in the following applications:
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris (pH 7.3), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for P4HA1 Antibody
2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase) 2-oxoglutarate-4-dioxygenase subunit alpha-1 4-PH alpha-1 alpha polypeptide I EC 22.214.171.124 Procollagen-proline prolyl 4-hydroxylase, alpha polypeptide I Background
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
for 1 year from date of receipt.
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Publications for P4HA1 Antibody (NB100-57852)(6)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 2 applications: IHC-P, WB.
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Publications using NB100-57852
Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014 [PMID: 25587023] (IHC-P, Human) IHC-P
Xiang L, Gilkes DM, Chaturvedi P, Semenza GL et al. Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer. J Mol Med (Berl). 2013 Nov 20 [PMID: 24248265]
Gilkes DM, Chaturvedi P, Bajpai S et al. Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res 2013 Jun 1 [PMID: 23539444] (WB, Human) WB
Gilkes DM, Bajpai S, Chaturvedi P et al. HIF-1 Promotes Extracellular Matrix Remodeling under Hypoxic Conditions by Inducing P4HA1, P4HA2, and PLOD2 Expression in Fibroblasts. J Biol Chem 2013 Feb 19 [PMID: 23423382] (WB, Human) WB
Van Duyn Graham L, Sweetwyne MT, Pallero MA, Murphy-Ullrich JE. Intracellular calreticulin regulates multiple steps in fibrillar collagen expression, trafficking, and processing into the extracellular matrix. J Biol Chem 2010 Mar 5 [PMID: 20044481]
Annunen P, Helaakoski T, Myllyharju J et al. Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer. J Biol Chem 1997 Jul 11 [PMID: 9211872]
P4HA1 Antibody (NB100-57852) (1)
Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
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Reviews using NB100-57852:
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Application Western Blot Sample Tested MDa-231, Sample Amount: 50ug Species Human Comments Excellent Antibody works well for Westerns, IHC, IF Blocking
Blocking Details Blocking Buffer: 5% milk in TBST, Blocking Time: 30 minutes, Blocking Temp: Room temperature Primary Anitbody
Dilution Ratio Dilution Ratio: 1:2500, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C Secondary Antibody
Secondary Description Secondary Ab: Santa Cruz Goat Secondary, Secondary Ab Dilution Ratio: 1:2500 Details
Detection Notes Detection Method: ECL Plus amersham, Exposure Time: 1 minute, Specific Bands: ~65kDa Comments
Comments Excellent Antibody works well for Westerns, IHC, IF
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