P4HA1 Antibody

Immunocytochemistry/Immunofluorescence: P4HA1 Antibody [NB100-57852] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852] - Staining of paraffin embedded Human Spleen with antibody at 7 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Bv, Ca
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

P4HA1 Antibody Summary

• Description: Novus Biologicals Goat P4HA1 Antibody (NB100-57852) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-P4HA1 Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This P4HA1 Antibody was developed against a peptide with sequence C-DFARKDEPDAFKE corresponding to internal region according to NP_001017962.1.
• Epitope: DFARKDEPDAFKE
• Specificity: This antibody is expected to recognize both reported isoforms (NP_000908.2 and NP_001017962.1).
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: P4HA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 7 ug/mL
  • Immunohistochemistry-Paraffin 7 ug/mL
  • Peptide ELISA Detection limit 1:128000
• Application Notes: IHC-P usage reported in scientific literature (PMID: 25587023).
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  293 Whole Cell Lysate
  P4HA1 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24993042).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for P4HA1 Antibody

• 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
• 2-oxoglutarate-4-dioxygenase subunit alpha-1
• 4-PH Alpha-1
• alpha polypeptide I
• EC 1.14.11.2
• P4HA
• P4HA1
• Procollagen-proline
• prolyl 4-hydroxylase, alpha polypeptide I

Background

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P4HA1 Antibody (NB100-57852)(9)

Publications using NB100-57852

• Eriksson J, Le Joncour V, Jahkola T et al. Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls Mol Oncol 2020-04-01 [PMID: 32053263] (WB, Human)
• Islam A, Fossum V, Hansen AK et al. In vitro chondrogenic potency of surplus chondrocytes from autologous transplantation procedures does not predict short-term clinical outcomes. BMC Musculoskelet Disord 2019-01-10 [PMID: 30630436] (WB, Human)
• Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014-01-01 [PMID: 25587023] (IHC-P, Human)
• Cowling RT, Yeo SJ, Kim IJ et al. Discoidin domain receptor 2 germline gene deletion leads to altered heart structure and function in the mouse Am J Physiol Heart Circ Physiol. 2014-07-02 [PMID: 24993042] (WB, Mouse)
• Xiang L, Gilkes DM, Chaturvedi P et al. Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer. J Mol Med (Berl). 2013-11-20 [PMID: 24248265]
• Gilkes DM, Chaturvedi P, Bajpai S et al. Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res 2013-06-01 [PMID: 23539444] (WB, Human)
• Gilkes DM, Bajpai S, Chaturvedi P et al. HIF-1 Promotes Extracellular Matrix Remodeling under Hypoxic Conditions by Inducing P4HA1, P4HA2, and PLOD2 Expression in Fibroblasts. J Biol Chem 2013-02-19 [PMID: 23423382] (WB, Human)
• Van Duyn Graham L, Sweetwyne MT, Pallero MA, Murphy-Ullrich JE. Intracellular calreticulin regulates multiple steps in fibrillar collagen expression, trafficking, and processing into the extracellular matrix. J Biol Chem 2010-03-05 [PMID: 20044481]
• Annunen P, Helaakoski T, Myllyharju J et al. Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer. J Biol Chem 1997-07-11 [PMID: 9211872]

Review for P4HA1 Antibody (NB100-57852) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/06/2010

Summary

• Application: Western Blot
• Sample Tested: MDa-231, Sample Amount: 50ug
• Species: Human

Bioinformatics

• Gene Symbol: P4HA1
• Entrez:
  • 5033(Human)
  • 18451(Mouse)
  • 64475(Rat)
• Uniprot:
  • P13674(Human)