P4HA1 Antibody

Images

 
Western Blot: P4HA1 Antibody [NB100-57852] - Analysis of P4HA1 in HEK293 cell lysate (35ug protein in RIPA buffer) using NB100-57852 at 0.1ug/ml.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852] - Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining
Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852] - Staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

P4HA1 Antibody Summary

Immunogen
Peptide with sequence C-DFARKDEPDAFKE corresponding to internal region according to NP_001017962.1.
Epitope
DFARKDEPDAFKE
Specificity
This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
P4HA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.1 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 60 kDa band observed in lysates of HEK293, A431 and NIH3T3 (calculated MW of 61.0 kDa band according to NP_000908.2 and NP_001017962.1). An additional band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P usage is reported in scientific literature (PMID: 25587023).
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
293 Lysate (NB800-PC6)
P4HA1 Lysate (NBL1-14050)
Reviewed Applications
Read 1 Review rated 5
using
NB100-57852 in the following applications:

Publications
Read Publications using
NB100-57852 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for P4HA1 Antibody

  • 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
  • 2-oxoglutarate-4-dioxygenase subunit alpha-1
  • 4-PH alpha-1
  • alpha polypeptide I
  • EC 1.14.11.2
  • Procollagen-proline
  • prolyl 4-hydroxylase, alpha polypeptide I

Background

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P4HA1 Antibody (NB100-57852)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(2)
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Human
(3)
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Showing Publications 1 - 6 of 6.
Publications using NB100-57852 Applications Species
Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014 [PMID: 25587023] (IHC-P, Human) IHC-P Human
Xiang L, Gilkes DM, Chaturvedi P, Semenza GL et al. Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer. J Mol Med (Berl). 2013 Nov 20 [PMID: 24248265]
Gilkes DM, Chaturvedi P, Bajpai S et al. Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res 2013 Jun 1 [PMID: 23539444] (WB, Human) WB Human
Gilkes DM, Bajpai S, Chaturvedi P et al. HIF-1 Promotes Extracellular Matrix Remodeling under Hypoxic Conditions by Inducing P4HA1, P4HA2, and PLOD2 Expression in Fibroblasts. J Biol Chem 2013 Feb 19 [PMID: 23423382] (WB, Human) WB Human
Van Duyn Graham L, Sweetwyne MT, Pallero MA, Murphy-Ullrich JE. Intracellular calreticulin regulates multiple steps in fibrillar collagen expression, trafficking, and processing into the extracellular matrix. J Biol Chem 2010 Mar 5 [PMID: 20044481]
Annunen P, Helaakoski T, Myllyharju J et al. Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer. J Biol Chem 1997 Jul 11 [PMID: 9211872]

Review for P4HA1 Antibody (NB100-57852) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-57852:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 08/06/2010
View

Summary

ApplicationWestern Blot
Sample TestedMDa-231, Sample Amount: 50ug
SpeciesHuman
CommentsExcellent Antibody works well for Westerns, IHC, IF

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 30 minutes, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:2500, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Santa Cruz Goat Secondary, Secondary Ab Dilution Ratio: 1:2500

Details

Detection NotesDetection Method: ECL Plus amersham, Exposure Time: 1 minute, Specific Bands: ~65kDa

Comments

CommentsExcellent Antibody works well for Westerns, IHC, IF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for P4HA1 Antibody (NB100-57852) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
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0
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2
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1
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Anonymous
08/06/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol P4HA1
Entrez
Uniprot