P4HA2 Antibody (CL0351) - BSA Free

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining in human epididymis and skeletal muscle tissues. Corresponding P4HA2 RNA-seq data are presented for the same tissues.

Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-P4HA2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human epididymis shows moderate to strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, Mycoplasma
• Clone: CL0351
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

P4HA2 Antibody (CL0351) - BSA Free Summary

• Description: Novus Biologicals Mouse P4HA2 Antibody (CL0351) - BSA Free (NBP2-52922) is a monoclonal antibody validated for use in IHC and WB. Anti-P4HA2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from O15460, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (90%), Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: P4HA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  P4HA2 Recombinant Protein Antigen (NBP2-52922PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for P4HA2 Antibody (CL0351) - BSA Free

• 2-oxoglutarate-4-dioxygenase subunit alpha-2
• 4-PH alpha 2
• 4-PH alpha-2
• alpha polypeptide II
• collagen prolyl 4-hydroxylase alpha(II)
• C-P4Halpha(II)
• EC 1.14.11.2
• Procollagen-proline
• procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
• prolyl 4-hydroxylase subunit alpha-2
• prolyl 4-hydroxylase, alpha polypeptide II

Background

P4HA2 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P4HA2 Antibody (NBP2-52922)(1)

Publication using NBP2-52922

• Zhang J, Lyu Z, Li B et al. P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases Hepatology (Baltimore, Md.) 2023-02-20 [PMID: 36799463] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: P4HA2