TGF-beta RIII Antibody

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PC‑3 human prostate cancer cell line was stained with Goat Anti-Human TGF‑ beta RIII Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF‑242‑PB, filled histogram) or isotype control antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

TGF-beta RIII Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RIII
Gly21-Asp781
Accession # AAA67061
Specificity
Detects human TGF-beta  RIII in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) TGF-beta  RIII is observed, and less than 1% cross-reactivity with recombinant human (rh) TGF-beta  RI, rhTGF-beta  RII, rhTGF-beta  RIIB, and rmTGF-beta  RI  is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFBR3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
Publications
Read Publications using
AF-242-PB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RIII Antibody

  • betaglycan proteoglycan
  • Betaglycan
  • BGCAN
  • TBRIII
  • TGF-beta receptor type 3
  • TGF-beta receptor type III
  • TGF-beta RIII
  • TGFbetaRIII
  • TGFBR3
  • TGF-bRIII
  • TGFR-3
  • Transforming growth factor beta receptor III
  • transforming growth factor beta receptor type 3
  • transforming growth factor, beta receptor III (betaglycan, 300kDa)
  • transforming growth factor, beta receptor III

Background

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta.

The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGF-beta RIII Antibody (AF-242-PB)(8)

We have publications tested in 3 confirmed species: Human, Rat, Rabbit.

We have publications tested in 6 applications: ELISA Development, Flow Cytometry, IHC, IHC-P, Immunoprecipitation, Neutralization.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(2)
IHC
(2)
IHC-P
(1)
Immunoprecipitation
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(5)
Rat
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF-242-PB Applications Species
Karthikeyan Mythreye Altering the Proteoglycan State of Transforming Growth Factor ? Type III Receptor (T?RIII)/Betaglycan Modulates Canonical Wnt/?-Catenin Signaling J. Biol. Chem., 2016;291(49):25716-25728. 2016 [PMID: 27784788] (Immunoprecipitation) Immunoprecipitation
Drasin D, Guarnieri A, Neelakantan D, Kim J, Cabrera J, Wang C, Zaberezhnyy V, Gasparini P, Cascione L, Huebner K, Tan A, Ford H TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation. Cancer Res, 2015;75(9):1908-21. 2015 [PMID: 25716682] (Flow Cytometry, Human) Flow Cytometry Human
Wang F, Zhou Y, Fu B, Wu Y, Zhang R, Sun R, Tian Z, Wei H Molecular signatures and transcriptional regulatory networks of human immature decidual NK and mature peripheral NK cells. Eur J Immunol, 2014;44(9):2771-84. 2014 [PMID: 24838931] (Flow Cytometry, Human) Flow Cytometry Human
Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration. Eur J Vasc Endovasc Surg, 2006;31(5):516-22. 2006 [PMID: 16427789] (IHC, Human) IHC Human
Florio P, Ciarmela P, Reis FM, Toti P, Galleri L, Santopietro R, Tiso E, Tosi P, Petraglia F Inhibin alpha-subunit and the inhibin coreceptor betaglycan are downregulated in endometrial carcinoma. Eur. J. Endocrinol., 2005;152(2):277-84. 2005 [PMID: 15745937] (IHC-P, Human) IHC-P Human
Rosado E, 2019, Schwartz Z, Sylvia VL, Dean DD, Boyan BD Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways. 91, 2002;1590(1):1-15. 2002 [PMID: 12063164] (Neutralization, Rat) Neutralization Rat
Bandyopadhyay A, 2019, Zhu Y, Malik SN, Kreisberg J, Brattain MG, Sprague EA, Luo J, Lopez-Casillas F, Sun LZ Extracellular domain of TGFbeta type III receptor inhibits angiogenesis and tumor growth in human cancer cells. 101205, 2002;21(22):3541-51. 2002 [PMID: 12032856] (ELISA Development, Human) ELISA Development Human
Song QH, Klepeis VE, Trinkaus-Randall V TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing. MP, Mol. Pathol., 2002;55(3):164-76. 2002 [PMID: 12032227] (IHC, Rabbit) IHC Rabbit

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF242
Phycoerythrin FAB242P

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Bioinformatics Tool for TGF-beta RIII Antibody (AF-242-PB)

Discover related pathways, diseases and genes to TGF-beta RIII Antibody (AF-242-PB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on TGF-beta RIII.

TGF-beta RIII - a high affinity reservoir for TGF-beta I/II ligands with therapeutic potential
TGF beta (transforming growth factor beta) is a superfamily of cytokines that participate in a variety of cellular processes including growth, proliferation, differentiation, and apoptosis. There are 3 classes of receptors for TGF beta cytokines an...  Read full blog post.

TGF-beta RIII - A multi-functional regulator of the TGF-beta signaling pathway
Transforming growth factor-beta receptor III (TGF-beta RIII) is one of three receptors for the secreted growth factor TGF-beta. Unlike type I and type II TGF-beta receptors, TGF-beta RIII does not participate directly in the propagation of intracel...  Read full blog post.

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Bioinformatics

Gene Symbol TGFBR3
Uniprot