P4HA1 Antibody

Images

 
Western Blot: P4HA1 Antibody [H00005033-B01P] - Analysis of P4HA1 expression in transfected 293T cell line by P4HA1 polyclonal antibody. Lane 1: P4HA1 transfected lysate(58.74 KDa). Lane 2: Non-transfected lysate.
Western Blot: P4HA1 Antibody [H00005033-B01P] - P4HA1 MaxPab polyclonal antibody. Western Blot analysis of P4HA1 expression in A-431.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

P4HA1 Antibody Summary

Immunogen
P4HA1 (NP_001017962.1, 1 a.a. - 534 a.a.) full-length human protein. MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE
Specificity
P4HA1 - procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
P4HA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 Review rated 5
using
H00005033-B01P in the following application:

Publications
Read Publications using H00005033-B01P.

Reactivity Notes

Rat reactivity reported in customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for P4HA1 Antibody

  • 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
  • 2-oxoglutarate-4-dioxygenase subunit alpha-1
  • 4-PH alpha-1
  • alpha polypeptide I
  • EC 1.14.11.2
  • Procollagen-proline
  • prolyl 4-hydroxylase, alpha polypeptide I

Background

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P4HA1 Antibody (H00005033-B01P)(2)

Review for P4HA1 Antibody (H00005033-B01P) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using H00005033-B01P:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Other 01/22/2010
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Summary

ApplicationWestern Blot
Sample TestedTaeniopygia guttata (Zebra Finch) Brain Tissue
SpeciesOther
CommentsThis antibody works in avian tissue. It consistently reveals a band of approximately 50kDa (the predicted mw for BMP2 in the zebra finch) in SDS PAGE blots. It also works well at a dilution of 1:500 for immunocytochemistry on tissue fixed with paraformaldehyde (4%). I have found that keeping the antibody out of the light as much as possible helps with its stability and longer term storage. My only complaint about the product is that it is shipped in solution rather than as a dessicate. I have found some variability in its effectiveness can arise from the shipping conditions.

Blocking

Blocking DetailsBlocking Buffer: 5% nonfat milk in TBS (for Westerns) and 10% normal serum (for ICCs), Blocking Time: 1 hour, Blocking Temp: RT

Primary Anitbody

Dilution RatioDilution Buffer: WB 1%NFM, 0.1%Tween20/TBS, ICC: 0.3%TritonX, 0.1M PB, Dilution Ratio: WB 1:500-1000, ICC 1:500, 48hrs at 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti mouse (preadsorbed), Secondary Ab Dilution: WB 1:1000, ICC 1:250 with ABC & DAB, IF 1:100

Details

Detection NotesVisualization Method: Fluorescence at 488nm, or DAB or Vector SG at the light level, Pos.Ctrl: Anti-HuC/HuD, Neg.Ctrl:No primary

Comments

CommentsThis antibody works in avian tissue. It consistently reveals a band of approximately 50kDa (the predicted mw for BMP2 in the zebra finch) in SDS PAGE blots. It also works well at a dilution of 1:500 for immunocytochemistry on tissue fixed with paraformaldehyde (4%). I have found that keeping the antibody out of the light as much as possible helps with its stability and longer term storage. My only complaint about the product is that it is shipped in solution rather than as a dessicate. I have found some variability in its effectiveness can arise from the shipping conditions.

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
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0
2
0
1
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Anonymous
01/22/2010
Application: WB
Species: Other

Bioinformatics

Gene Symbol P4HA1
Entrez
Uniprot