Mouse IgG Isotype Control

Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]

SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - SDS-Page of Mouse IgG whole molecule Lane 1: Mouse IgG reduced Lane 3: Mouse IgG non-reduced Load: 1 ug per lane Predicted/Observed size (non-reduced): 160 kDa, 160 kDa Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa

SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - Lane 1: 5 uL Opal Prestained Marker Lane 2: Reduced Mouse IgG Whole Molecule (BULK ORDER) Lane 3: Reduced Mouse F(c) Fragment Lane 4: Reduced Mouse F(ab) Fragment Lane 5: Mouse IgM Kappa Myeloma Protein Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, IHC, IP, PAGE
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Mouse IgG Isotype Control Summary

• Description: This Mouse IgG immunoglobulin is useful as an isotype control for Mouse IgG antibody.
• Isotype: IgG
• Host: Mouse
• Gene: IGHG1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • SDS-Page
  • Western Blot
• Application Notes: This isotype control can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 500 ul deionized water (or equivalent).

Notes

This product was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.

Store at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Mouse IgG Isotype Control

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (NBP1-97019)(2)

Publications using NBP1-97019

• Chittezhath M, Wai CMM, Tay VSY et al. TLR4 signals through islet macrophages to alter cytokine secretion during diabetes J. Endocrinol. Oct 1 2020 [PMID: 32755994] (Mouse)
• Yang X, Balakrishnan I, Torok-Storb B, Pillai MM. Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion Adv Hematol 2012 [PMID: 22400029] (IHC, Mouse)

Reviews for IgG Isotype Control (NBP1-97019) (2)

Average Rating: 5

(Based on 2 reviews)

reviewed by: Verified Customer ICC/IF 04/13/2018

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Lot: 39854

reviewed by: Verified Customer 04/13/2018

Summary

• Application: Serum
• Lot: 39854

Bioinformatics

• Gene Symbol: IGHG1