Mouse IgG Isotype Control

Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]

SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - Lane 1: 5 uL Opal Prestained Marker Lane 2: Reduced Mouse IgG Whole Molecule (BULK ORDER) Lane 3: Reduced Mouse F(c) Fragment Lane 4: Reduced Mouse F(ab) Fragment Lane 5: Mouse IgM Kappa Myeloma Protein Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - SDS-Page of Mouse IgG Isotype control. Lane 1: Mouse IgG reduced. Lane 2: Molecular Weight Marker. Lane 3: Mouse IgG non-reduced. Load: 1 ug per lane.Predicted/Observed size (non-reduced): 160 kDa, 160 kDa.Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, IHC, IP, PAGE
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Mouse IgG Isotype Control Summary

• Description: This product was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.
  
  Store at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Isotype: IgG
• Host: Mouse
• Gene: IGHG1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • SDS-Page
  • Western Blot
• Application Notes: This product has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 500 ul deionized water (or equivalent).

Alternate Names for Mouse IgG Isotype Control

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (NBP1-97019)(5)

Publications using NBP1-97019

• Lin A, Shen H, Spychalski G et al. Parallelized immunomagnetic nanopore sorting: modeling, scaling, and optimization of surface marker specific isolation of extracellular vesicles from complex media Research Square 2023-05-16 [PMID: 37292737] (FLOW)
• Wang R, Nissen NN, Zhang Y Et al. Circulating Fatty Objects and Their Preferential Presence in Pancreatic Cancer Patient Blood Samples Front Physiol 2022-03-03 [PMID: 35237181]
  
  Details:
  Citation using the Biotin version of this antibody.
• Li J, Conrad C, Mills TW et al. PMN-derived netrin-1 attenuates cardiac ischemia-reperfusion injury via myeloid ADORA2B signaling The Journal of experimental medicine 2021-06-07 [PMID: 33891683] (Immunohistochemistry, Mouse)
• Chittezhath M, Wai CMM, Tay VSY et al. TLR4 signals through islet macrophages to alter cytokine secretion during diabetes J. Endocrinol. 2020-10-01 [PMID: 32755994] (Mouse)
• Yang X, Balakrishnan I, Torok-Storb B, et al. Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion Adv Hematol 2012-03-09 [PMID: 22400029] (IF/IHC, Mouse)

Review for IgG Isotype Control (NBP1-97019) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 04/13/2018

Summary

• Lot: 39854

Bioinformatics

• Gene Symbol: IGHG1