![Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]](http://images.novusbio.com/fullsize2/Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP1-97019-img0004.jpg "Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, IHC-P, IP, PAGE |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Purified |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Agarose | NBP1-97142 | |||
| Alkaline Phosphatase | NBP1-97220 | |||
| Biotin | NBP1-97078 | |||
| FITC | NBP1-96789 | |||
| HRP | NBP1-97073 | |||
| Rhodamine | NBP1-96790 | |||
| Texas Red | NBP1-97079 | |||
| Description | This Mouse IgG immunoglobulin is useful as an isotype control for Mouse IgG antibody. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum. |
| Isotype | IgG |
| Host | Mouse |
| Gene | IGHG1 |
| Purity | Ion exchange chromatography |
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| Application Notes | NBP1-97019 is in a liquid physical state; NBP1-97019-5mg is lyophilized. |
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| Storage | Store at 4C. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Ion exchange chromatography |
| Reconstitution Instructions | For the 5mg size, we recommend reconstitution with 0.5 ml of dH2O. The 1mg size is provided in liquid format (0.1 ml) |
| Publication using NBP1-97019 | Applications | Species |
|---|---|---|
| Yang X, Balakrishnan I, Torok-Storb B, Pillai MM. Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion Adv Hematol 2012 [PMID: 22400029] (IHC, Mouse) | IHC | Mouse |
| Images | Ratings | Applications | Species | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Anonymous |
ICC/IF | 04/13/2018 |
Summary
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Enlarge |
reviewed by:
Anonymous |
04/13/2018 |
Summary
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| Agarose | NBP1-97142 | |
| Alkaline Phosphatase | NBP1-97220 | |
| Biotin | NBP1-97078 | |
| FITC | NBP1-96789 | |
| HRP | NBP1-97073 | |
| Rhodamine | NBP1-96790 | |
| Texas Red | NBP1-97079 |
Diseases for IgG Isotype Control (NBP1-97019)Discover more about diseases related to IgG Isotype Control (NBP1-97019).
| Pathways for IgG Isotype Control (NBP1-97019)View related products by pathway.
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PTMs for IgG Isotype Control (NBP1-97019)Learn more about PTMs related to IgG Isotype Control (NBP1-97019).
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CD16 - Find me on macrophages, neutrophils and NK cells CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells.... Read full blog post. |
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Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Anonymous 04/13/2018 |
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| Application: | ICC/IF | |
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| Anonymous 04/13/2018 |
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| Gene Symbol | IGHG1 |
![SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - SDS-Page of Mouse IgG whole molecule Lane 1: Mouse IgG reduced Lane 3: Mouse IgG non-reduced Load: 1 ug per lane Predicted/Observed size (non-reduced): 160 kDa, 160 kDa Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa](http://images.novusbio.com/fullsize2/Mouse-IgG-Isotype-Control-SDS-Page-NBP1-97019-img0002.jpg "SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - SDS-Page of Mouse IgG whole molecule Lane 1: Mouse IgG reduced Lane 3: Mouse IgG non-reduced Load: 1 ug per lane Predicted/Observed size (non-reduced): 160 kDa, 160 kDa Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa")
![SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - Lane 1: 5 uL Opal Prestained Marker Lane 2: Reduced Mouse IgG Whole Molecule (BULK ORDER) Lane 3: Reduced Mouse F(c) Fragment Lane 4: Reduced Mouse F(ab) Fragment Lane 5: Mouse IgM Kappa Myeloma Protein Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize2/Mouse-IgG-Isotype-Control-SDS-Page-NBP1-97019-img0003.jpg "SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - Lane 1: 5 uL Opal Prestained Marker Lane 2: Reduced Mouse IgG Whole Molecule (BULK ORDER) Lane 3: Reduced Mouse F(c) Fragment Lane 4: Reduced Mouse F(ab) Fragment Lane 5: Mouse IgM Kappa Myeloma Protein Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.")
![Western Blot: RAG1 Antibody [NBP1-74190] - Mouse Liver Lysate 1ug/ml Gel Concentration 6-18%](http://images.novusbio.com/fullsize/RAG1 Antibody-Western Blot-NBP1-74190-img0001.jpg)
![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Flow-(Intracellular)-NB200-540-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
![Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/AICDA Antibody-Western Blot-NBP1-30955-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-30955] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/AICDA Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30955-img0002.jpg)
![Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded mouse lymph node. AICDA antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/fullsize2/AICDA-Antibody-Immunohistochemistry-Paraffin-NBP1-30955-img0005.jpg)
![Western Blot: SPANXB1 Antibody [H00728695-B01P] - Analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate(11.33 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/SPANXB1 Antibody-Western Blot-H00728695-B01P-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SPANXB1 Antibody [H00728695-B01P] - Analysis of purified antibody to SPANXB1 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize2/SPANXB1-Antibody-Immunocytochemistry-Immunofluorescence-H00728695-B01P-img0003.jpg)
![Western Blot: BBS9 Antibody [NBP1-88927] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize2/BBS9-Antibody-Western-Blot-NBP1-88927-img0004.jpg)
![Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize2/BBS9-Antibody-Immunohistochemistry-Paraffin-NBP1-88927-img0002.jpg)
![Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Controls run before the experimental samples are analyzed, to adjust the fluorescent compensation on the flow cytometer due to the FITC signal from the Annexin V-FITC bleeding into the Propidium Iodide signal.](http://images.novusbio.com/fullsize2/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0003.jpg)
![Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Adherent RAW cells treated with actinomycin-D, detached with a cell detachment solution and stained with the Annexin V Apoptosis Kit [FITC].](http://images.novusbio.com/fullsize2/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0001.jpg)
![Western Blot: RAG2 Antibody [NBP2-43666] - Analysis of A. 50 ug rat thymus lysate/extract 7.5 % SDS-PAGE RAG2 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize2/RAG2-Antibody-Western-Blot-NBP2-43666-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RAG2 Antibody [NBP2-43666] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAG2 protein stained by RAG2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10um.](http://images.novusbio.com/fullsize2/RAG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-43666-img0005.jpg)
![Immunohistochemistry-Paraffin: RAG2 Antibody [NBP2-43666] - Analysis of DLD1 Xenograft, using RAG2 antibody at 1:100 dilution.](http://images.novusbio.com/fullsize2/RAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-43666-img0002.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-87404-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Adenosine-Deaminase-ADA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87404-img0013.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human colon, duodenum, lymph node and testis using Anti-ADA antibody NBP1-87404 (A) shows similar protein distribution across tissues to independent antibody NBP1-90361 (B).](http://images.novusbio.com/fullsize2/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0023.jpg)
![Immunohistochemistry-Paraffin: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - IgA2 stained human tonsil FFPE, 20X magnification. Antigen retrieval was performed with citrate pH 6 buffer. Primary antibody at 1:8000. Image from verified customer reivew.](http://images.novusbio.com/fullsize2/IgA2-Antibody-(RM125)-[Unconjugated]-Immunohistochemistry-Paraffin-NBP2-62025-img0005.jpg)
![Immunohistochemistry: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - Immunohistochemistry of Human Tonsil Tissue using NBP2-62025.](http://images.novusbio.com/fullsize2/IgA2-Antibody-(RM125)-[Unconjugated]-Immunohistochemistry-NBP2-62025-img0004.jpg)
![ELISA: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - ELISA of human immunoglobulins shows NBP2-62025 only reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with different immunoglobulins.NBP2-62025 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/IgA2-Antibody-(RM125)-[Unconjugated]-ELISA-NBP2-62025-img0006.jpg)
![Western Blot: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Analysis of Haptoglobin on different cells lysates using anti-Haptoglobin antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: HepG2](http://images.novusbio.com/fullsize2/Haptoglobin Antibody (JM10-79)-Western Blot-NBP2-66881-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Staining Haptoglobin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Haptoglobin-Antibody-(JM10-79)-Immunocytochemistry-Immunofluorescence-NBP2-66881-img0003.jpg)
![Immunohistochemistry-Paraffin: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Analysis of paraffin-embedded mouse lung tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Haptoglobin-Antibody-(JM10-79)-Immunohistochemistry-Paraffin-NBP2-66881-img0007.jpg)
![Western Blot: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Immunoblot of Balb/c thymus (lane 1) and A20 (lane 2) cell lysates with Anti-Mouse CD20 Purified.](http://images.novusbio.com/fullsize2/MS4A1-CD20-Antibody-(AISB12)-Western-Blot-NBP1-43435-img0002.jpg)
![Flow Cytometry: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor (R) 647 and 1.0 micrograms conjugated anti-Mouse CD20 Purified followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize2/MS4A1-CD20-Antibody-(AISB12)-Flow-Cytometry-NBP1-43435-img0004.jpg)
![Western Blot: PGK1 Antibody [NBP2-19784] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/PGK1-Antibody-Western-Blot-NBP2-19784-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PGK1 Antibody [NBP2-19784] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGK1 protein stained by PGK1 antibody diluted at 1:20. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/PGK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19784-img0014.jpg)
![Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/PGK1-Antibody-Immunohistochemistry-Paraffin-NBP2-19784-img0008.jpg)
![Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Controls run before the experimental samples are analyzed, to adjust the fluorescent compensation on the flow cytometer due to the FITC signal from the Annexin V-FITC bleeding into the Propidium Iodide signal.](http://images.novusbio.com/images2/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0003.jpg "Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Controls run before the experimental samples are analyzed, to adjust the fluorescent compensation on the flow cytometer due to the FITC signal from the Annexin V-FITC bleeding into the Propidium Iodide signal.")