Mouse IgG Isotype Control

Images

 
Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]
SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - SDS-Page of Mouse IgG whole molecule Lane 1: Mouse IgG reduced Lane 3: Mouse IgG non-reduced Load: 1 ug per lane Predicted/Observed size (non-reduced): 160 kDa, 160 kDa ...read more
SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - Lane 1: 5 uL Opal Prestained Marker Lane 2: Reduced Mouse IgG Whole Molecule (BULK ORDER) Lane 3: Reduced Mouse F(c) Fragment Lane 4: Reduced Mouse F(ab) Fragment Lane ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IP, PAGE
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Mouse IgG Isotype Control Summary

Description
This Mouse IgG immunoglobulin is useful as an isotype control for Mouse IgG antibody.
Isotype
IgG
Host
Mouse
Gene
IGHG1
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • SDS-Page
  • Western Blot
Application Notes
This isotype control can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-97019 in the following applications:

Publications
Read Publications using
NBP1-97019 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 500 ul deionized water (or equivalent).

Notes

This product was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.

Store at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Mouse IgG Isotype Control

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Publications for IgG Isotype Control (NBP1-97019)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
Mouse
(2)
All Species

Reviews for IgG Isotype Control (NBP1-97019) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using NBP1-97019:
Filter by Applications
ICC/IF
(1)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence IgG NBP1-97019
Enlarge
5
reviewed by:
Verified Customer
ICC/IF 04/13/2018
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Lot39854
Serum IgG NBP1-97019
Enlarge
5
reviewed by:
Verified Customer
04/13/2018
View

Summary

ApplicationSerum
Lot39854

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Isotype Control (NBP1-97019) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Agarose NBP1-97142
Alkaline Phosphatase NBP1-97220
Biotin NBP1-97078
FITC NBP1-96789
HRP NBP1-97073
Rhodamine NBP1-96790
Texas Red NBP1-97079

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Blogs on IgG.

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Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


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CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
04/13/2018
Application: ICC/IF
Species:

Verified Customer
04/13/2018
Application:
Species:

Bioinformatics

Gene Symbol IGHG1