Mouse IgG Isotype Control

Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]

SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - SDS-Page of Mouse IgG whole molecule Lane 1: Mouse IgG reduced Lane 3: Mouse IgG non-reduced Load: 1 ug per lane Predicted/Observed size (non-reduced): 160 kDa, 160 kDa Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa

SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - Lane 1: 5 uL Opal Prestained Marker Lane 2: Reduced Mouse IgG Whole Molecule (BULK ORDER) Lane 3: Reduced Mouse F(c) Fragment Lane 4: Reduced Mouse F(ab) Fragment Lane 5: Mouse IgM Kappa Myeloma Protein Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, IHC, IHC-P, IP, PAGE
• Host: Mouse
• Conjugate: Unconjugated
• Format: Purified

Mouse IgG Isotype Control Summary

• Description: This Mouse IgG immunoglobulin is useful as an isotype control for Mouse IgG antibody.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.
• Isotype: IgG
• Host: Mouse
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500SDS-PAGE 1:100-1:2000
  • SDS-Page 1:100-1:2000
• Application Notes: NBP1-97019 is in a liquid physical state; NBP1-97019-5mg is lyophilized.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Ion exchange chromatography
• Reconstitution Instructions: For NBP1-97019-5mg: Reconstitute with 500 ul of deionized water (or equivalent).

Alternate Names for Mouse IgG Isotype Control

• IgG
• immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (NBP1-97019)(1)

Publication using NBP1-97019

• Yang X, Balakrishnan I, Torok-Storb B, Pillai MM. Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion Adv Hematol 2012 [PMID: 22400029] (IHC, Mouse)

Review for IgG Isotype Control (NBP1-97019) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Anonymous ICC/IF 04/13/2018

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Lot: 39854