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ORP150/HSP12A Antibody (201) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
201
Clonality
Monoclonal
Host
Rabbit
Conjugate
Alexa Fluor 700

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-89890AF350
Alexa Fluor 405
NBP2-89890AF405
Alexa Fluor 488
NBP2-89890AF488
Alexa Fluor 532
NBP2-89890AF532
Alexa Fluor 594
NBP2-89890AF594
Alexa Fluor 647
NBP2-89890AF647
Alexa Fluor 750
NBP2-89890AF750
Biotin
NBP2-89890B
DyLight 350
NBP2-89890UV
DyLight 405
NBP2-89890V
DyLight 488
NBP2-89890G
DyLight 550
NBP2-89890R
DyLight 594
NBP2-89890DL594
DyLight 650
NBP2-89890C
DyLight 680
NBP2-89890FR
DyLight 755
NBP2-89890IR
FITC
NBP2-89890F
HRP
NBP2-89890H
Janelia Fluor 549
NBP2-89890JF549
Janelia Fluor 646
NBP2-89890JF646
Unconjugated
NBP2-89890-100ul
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ORP150/HSP12A Antibody (201) [Alexa Fluor® 700] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human ORP150/HSP12A (NP_001124463.1; Met 695-Leu 994).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HYOU1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ORP150/HSP12A Antibody (201) [Alexa Fluor® 700]

  • 150 kDa oxygen-regulated protein
  • 170 kDa glucose-regulated protein
  • DKFZp686N08236
  • FLJ97572
  • glucose-regulated protein 170
  • Grp170
  • GRP-170
  • HSP12A
  • HYOU1
  • hypoxia up-regulated 1
  • hypoxia up-regulated protein 1
  • ORP150
  • ORP-150
  • ORP150FLJ94899
  • oxygen regulated protein (150kD)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - C. elegans granulins impair organismal fitness and resistance to ER stress. Total worm lysates from synchronized day 1 adult granulin-expressing animals were immunoblotted with an anti-GRP78/HSPA5 antibody (3 biological replicates). Anti-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1008295), licensed under a CC-BY licence.
Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis of MAM-associated protein levels in control and patients' fibroblasts.(A) Equal amounts from one control and the five patients were loaded (50 ug of total cell lysates) and subjected to Western Blot with anti-Grp75, anti-sigma-1R and anti-Mfn2 antibodies. We used anti-GAPDH antibody to ensure equal amounts of protein loaded in each lane. This result is representative of three independent experiments. PLoS One. 2016 Mar 9;11(3):e0150357. doi: 10.1371/journal.pone.0150357
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Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates.
Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - GR94 in human primary fibroblasts, ER staining. Primary antibody incubation: 1:250 in 10% Serum in PBST (0.1% Triton) O/N 4C. Secondary: Donkey anti-rat 647 1:500 2h RT. ICC/IF image submitted by a verifed customer review.
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse breast tissue.
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Western Blot: HspA4 Antibody (SD0861) [NBP2-67817] - Analysis of HSPA4 on Hela cells lysates using anti-HSPA4 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: HspA4 Antibody (SD0861) [NBP2-67817] - Staining HSPA4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: HspA4 Antibody (SD0861) [NBP2-67817] - Analysis of paraffin-embedded rat brain tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
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NBP2-16630
Western Blot: BMP-3b/GDF-10 Antibody [NBP2-16630] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:500
Immunocytochemistry/Immunofluorescence: BMP-3b/GDF-10 Antibody [NBP2-16630] - DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: GDF10 protein stained by GDF10 antibody [N3C3] diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: BMP-3b/GDF-10 Antibody [NBP2-16630] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:500
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
HSPH1/HSP105
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NBP2-67822
Western Blot: HSPH1/HSP105 Antibody (SD85-06) [NBP2-67822] - Analysis of Hsp105 on different lysates using anti-Hsp105 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela
Immunocytochemistry/Immunofluorescence: HSPH1/HSP105 Antibody (SD85-06) [NBP2-67822] - Staining Hsp105 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: HSPH1/HSP105 Antibody (SD85-06) [NBP2-67822] - Analysis of paraffin-embedded rat testis tissue using anti-Hsp105 antibody. Counter stained with hematoxylin.
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ERp57/PDIA3
Antibody
NBP1-84796
Western Blot: ERp57/PDIA3 Antibody [NBP1-84796] - Analysis using Anti-PDIA3 antibody NBP1-84796 (A) shows similar pattern to independent antibody NBP1-84797 (B).
Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NBP1-84796] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84796] - Staining in human thyroid gland and skeletal muscle tissues using NBP1-84796 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.
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VEGF [HRP]
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Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using NB600-101. Secondary antibody was conjugated with Alexa Fluor 488. Photo courtesy of Dr. Birkenkamp Demtroeder, Arhus University Hospital.
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Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77685] - HSP901a antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
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Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
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Immunohistochemistry: Calnexin Antibody [NB100-1965] - Calnexin immunostaining in the anterior (AI) and mid (MI) intestine of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5-T2 (D.I.O. zebrafish followed by 3,5-T2), D.I.O. with 3,5-T2 (D.I.O. zebrafish treated with 3,5-T2). (A) AI of ctrl zebrafish (B) AI of D.I.O. (C) AI of D.I.O. flw 3,5-T2. (D) AI of D.I.O. with 3,5-T2. The arrows indicate calnexin immunoexpression in the enteroendocrine and goblet cells. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2076-2615/10/7/1131/htm#) licensed under a CC-BY licence.
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Immunocytochemistry/Immunofluorescence: ATF6 Antibody (70B1413.1) [NBP1-40256] - Untreated HeLa cells were fixed in -20C methanol for 10 min, air dried and rehydrated in PBS at room temperature for 5 minutes. Cells were incubated with anti-ATF6 (1:20) for one hour at room temperature. ATF6 reactivity (green) was detected with anti-mouse Dylight-488 secondary antibody. Nuclei were counterstained with DAPI (blue). Note the ER localization of ATF6.
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Caspase-3 Antibody
(31A1067) - (Pro and ...
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Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
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HSP60 Antibody
NBP1-77397
Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of extracts from HeLa and COS cells using NBP1-77397 Hsp60 antibody at 1:1000
Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
Immunohistochemistry: HSP60 Antibody [NBP1-77397] - Staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.
(4 Reviews)
(3 Publications)
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Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, KD
ERp72 Antibody
(10-F2)
NBP2-76846
Western Blot: ERp72 Antibody (10-F2) [NBP2-76846] - Western blot analysis of ERp72 on A431 cell lysate using anti-ERp72 antibody at 1/500 dilution.
Immunocytochemistry/Immunofluorescence: ERp72 Antibody (10-F2) [NBP2-76846] - ICC staining ERp72 (green) in SKOV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ERp72 Antibody (10-F2) [NBP2-76846] - Analysis of mouse brain tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
HERPUD1 Antibody
(OTI1A2) - Azide and ...
NBP2-71492
Western Blot: HERPUD1 Antibody (OTI1A2) - Azide and BSA Free [NBP2-71492] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HERPUD1.
Immunocytochemistry/Immunofluorescence: HERPUD1 Antibody (OTI1A2) - Azide and BSA Free [NBP2-71492] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HERPUD1.
Immunohistochemistry: HERPUD1 Antibody (OTI1A2) - Azide and BSA Free [NBP2-71492] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC
SIL1 Antibody
(OTI3E3)
NBP2-02377
Western Blot: SIL1 Antibody (3E3) [NBP2-02377] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIL1.
Immunocytochemistry/Immunofluorescence: SIL1 Antibody (3E3) [NBP2-02377] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1.
Immunohistochemistry-Paraffin: SIL1 Antibody (3E3) [NBP2-02377] - Staining of paraffin-embedded liver tissue using anti-SIL1 mouse monoclonal antibody.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Protein Disulfide
Isomerase/P4HB Antibo ...
NBP3-03678
Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Analysis of extracts of various cell lines, using Protein Disulfide Isomerase/P4HB antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.w
Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Analysis of U-2OS cells using Protein Disulfide Isomerase/P4HB antibody at dilution of 1:400. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Rat kidney using P4HB antibody at dilution of 1:150 (40x lens).
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, KO
ORP150/HSP12A
Antibody (201) [Alexa ...
NBP2-89890AF700
ORP150/HSP12A Antibody (201) [Alexa Fluor® 700]
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

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Product General Protocols

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Video Protocols

WB Video Protocol
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FAQs for ORP150/HSP12A Antibody (NBP2-89890AF700) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-89890AF350
Alexa Fluor 405 NBP2-89890AF405
Alexa Fluor 488 NBP2-89890AF488
Alexa Fluor 532 NBP2-89890AF532
Alexa Fluor 594 NBP2-89890AF594
Alexa Fluor 647 NBP2-89890AF647
Alexa Fluor 700 NBP2-89890AF700
Alexa Fluor 750 NBP2-89890AF750
Biotin NBP2-89890B
DyLight 350 NBP2-89890UV
DyLight 405 NBP2-89890V
DyLight 488 NBP2-89890G
DyLight 550 NBP2-89890R
DyLight 594 NBP2-89890DL594
DyLight 650 NBP2-89890C
DyLight 680 NBP2-89890FR
DyLight 755 NBP2-89890IR
FITC NBP2-89890F
HRP NBP2-89890H
Janelia Fluor 549 NBP2-89890JF549
Janelia Fluor 646 NBP2-89890JF646
Learn about Custom Antibody Labeling

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Bioinformatics Tool for ORP150/HSP12A Antibody (NBP2-89890AF700)

Discover related pathways, diseases and genes to ORP150/HSP12A Antibody (NBP2-89890AF700). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for ORP150/HSP12A Antibody (NBP2-89890AF700)

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Pathways for ORP150/HSP12A Antibody (NBP2-89890AF700)

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PTMs for ORP150/HSP12A Antibody (NBP2-89890AF700)

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Bioinformatics

Gene Symbol HYOU1