Optineurin Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Optineurin Antibody [NBP1-84683] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683] - Staining of human rectum shows moderate cytoplasmic positivity in cells in granular layer.

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Analysis in human cell lines A-431 and HEK293 using Anti-OPTN antibody. Corresponding OPTN RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Analysis using Anti-OPTN antibody HPA003279 (A) shows similar pattern to independent antibody HPA003360 (B).

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Optineurin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Optineurin Antibody - BSA Free (NBP1-84683) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OPTN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Optineurin Protein (NBP1-84683PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Optineurin Antibody - BSA Free

• ALS12
• FIP2
• FIP2FIP-2
• GLC1E
• GLC1Eglaucoma 1, open angle, E (adult-onset)
• HIP7
• HIP7ALS12
• Huntingtin interacting protein L
• Huntingtin yeast partner L
• Huntingtin-interacting protein 7
• Huntingtin-interacting protein L
• HYPL
• HYPLHIP-7
• NEMO-related protein
• NRP
• NRPE3-14.7K-interacting protein
• Optic neuropathy-inducing protein
• Optineurin
• OPTN
• TFIIIA-INTP
• Transcription factor IIIA-interacting protein
• transcrption factor IIIA-interacting protein
• tumor necrosis factor alpha-inducible cellular protein containing leucinezipper domains

Background

Optineurin encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OPTN