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NELL1 Antibody

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Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse NELL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7109) followed by ...read more
NELL1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse NELL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7109) at 5 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

NELL1 Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse NELL1
Arg17-Asn810
Accession # Q2VWQ2
Specificity
Detects mouse NELL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human NELL1 is observed and approximately 10% cross-reactivity with recombinant mouse NELL2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NELL1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NELL1 Antibody

  • FLJ45906
  • IDH3GL
  • nel (chicken)-like 1
  • NELL1
  • NEL-like 1 (chicken)
  • NEL-like protein 1
  • Nel-related protein 1
  • neural epidermal growth factor-like 1
  • NRP1
  • protein kinase C-binding protein NELL1

Background

NELL1 (neural EGF-like like protein 1) is an 89 kDa (predicted) member of the EGF-like domain containing family, Laminin G/N-TSP1/Pentraxin gene superfamily of molecules. When secreted, NELL1 exists as a phosphoglycoprotein that can add as much as 50 kDa to the calculated MW. NELL1 has restricted expression, being limited to pre-B cells and osteoblasts, where it apparently promotes osteoblast maturation and bone formation. In tumors, it is found in neuroblastoma-derived cells. NELL1 is both secreted and retained intracellularly where it is phosphorylated by PKC. The mouse NELL1 precursor is 810 amino acids (aa) in length. It contains a 16 aa signal sequence plus a 794 aa mature region. The mature region possesses an N-terminal TSP domain (aa 81-230), two VWFC domains (aa 271-390), six consecutive EGF-like domains (aa 391-631), and three additional C-terminal VWFC domains (aa 632-807). Secreted NELL1 forms a 400-420 kDa noncovalent homotrimer. Over aa #17-810, mouse NELL1 shares 98% and 93% aa identity with rat and human NELL1, respectively.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NELL1
Uniprot