Myocilin Antibody (297817) [Unconjugated]

Myocilin was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human Myocilin Monoclonal Antibody (Catalog # MAB3446) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm and sarcolemma in skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 297817
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Myocilin Antibody (297817) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Myocilin
  Arg33-Met504
  Accession # Q99972
• Specificity: Detects human Myocilin in direct ELISAs. In direct ELISAs, 75% cross-reactivity with recombinant mouse Myocilin is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYOC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myocilin Antibody (297817) [Unconjugated]

• GLC1A
• GLC1Amyocilin
• GPOA
• JOAG
• JOAG1
• MYOC
• Myocilin
• myocilin, trabecular meshwork inducible glucocorticoid response
• TIGR
• TIGRmutated trabecular meshwork-induced glucocorticoid response protein
• Trabecular meshwork-induced glucocorticoid response protein

Background

Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 aa precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha ‑helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat, and mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myocilin Antibody (MAB3446)(7)

Publications using MAB3446

• HF Scelsi, KR Hill, BM Barlow, MD Martin, RL Lieberman Disambiguation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability Disease Models & Mechanisms, 2023-01-13;0(0):. 2023-01-13 [PMID: 36579626] (Western Blot, Human)
• JV Jasien, AT Read, J van Batenb, KM Perkumas, CR Ethier, WD Stamer, BC Samuels Anterior Segment Anatomy and Conventional Outflow Physiology of the Tree Shrew (Tupaia belangeri) Investigative Ophthalmology & Visual Science, 2022-01-03;63(1):21. 2022-01-03 [PMID: 35040876] (IHC, Tree Shrew)
• AC Patterson-, AN Qerqez, LR Azouz, MT Ma, SE Hill, Y Ku, LA Schildmeye, JA Maynard, RL Lieberman Recombinant antibodies recognize conformation-dependent epitopes of the leucine zipper of misfolding-prone myocilin The Journal of Biological Chemistry, 2021-08-09;0(0):101067. 2021-08-09 [PMID: 34384785] (Western Blot, Human)
• R Haribalaga, C Gowri Priy, R Sharmila, S Krishnadas, V Muthukkaru, CE Willoughby, S Senthilkum Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes Scientific Reports, 2021-01-12;11(1):605. 2021-01-12 [PMID: 33436790] (IHC, Human)
• SE Hill, MS Kwon, MD Martin, A Suntharali, A Hazel, CA Dickey, JC Gumbart, RL Lieberman Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between�benign and glaucoma-causing mutations J. Biol. Chem., 2019-07-02;0(0):. 2019-07-02 [PMID: 31270212] (Western Blot, Human)
• Suntharalingam A, Abisambra J, O'Leary J, Koren J, Zhang B, Joe M, Blair L, Hill S, Jinwal U, Cockman M, Duerfeldt A, Tomarev S, Blagg B, Lieberman R, Dickey C Glucose-regulated protein 94 triage of mutant myocilin through endoplasmic reticulum-associated degradation subverts a more efficient autophagic clearance mechanism. J Biol Chem, 2012-10-03;287(48):40661-9. 2012-10-03 [PMID: 23035116] (Immunoprecipitation, Human)
• Joe MK, Tomarev SI Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis. Am. J. Pathol., 2010-04-09;176(6):2880-90. 2010-04-09 [PMID: 20382707] (Western Blot, Human)

Review for Myocilin Antibody (MAB3446) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Pig.

reviewed by: L P ICC Pig 01/04/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Pig TM cells
• Species: Pig

Comments

• Comments: IHC showing detection of Myocilin using anti-Myocilin antibody (MAB3446) at 1:1000 dilution in Pig TM cells.

Bioinformatics

• Gene Symbol: MYOC
• Uniprot:
  • Q99972()