TDP-43/TARDBP Antibody (2E2-D3)

Images

 
Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP monoclonal antibody (M01), clone 2E2-D3 Analysis of TARDBP expression in A-431.
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of monoclonal antibody to TARDBP on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of monoclonal antibody to TARDBP on formalin-fixed paraffin-embedded human leiomyosarcoma tissue Antibody concentration 3 ug/ml.
Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi ( Cat # H00023435-R01V ) (Lane 2) or non-transfected ...read more
Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of TARDBP expression in transfected 293T cell line by TARDBP monoclonal antibody (M01), clone 2E2-D3.Lane 1: TARDBP transfected lysate(44.7 ...read more
Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP in rat tissue samples. Image courtsey of product review by Daniel Pirici.
Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]
Immunoprecipitation: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of TARDBP transfected lysate using anti-TARDBP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TARDBP MaxPab rabbit ...read more
ELISA: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Detection limit for recombinant GST tagged TARDBP is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
2E2-D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TDP-43/TARDBP Antibody (2E2-D3) Summary

Immunogen
TARDBP (AAH01487, 1 a.a. ~ 260 a.a) full length recombinant protein with GST tag. Molecular weight of GST Tag is 26KDa alone. MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISV
Localization
H00023435-M01 Nucleus.
Specificity
TARDBP - TAR DNA binding protein
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TARDBP
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), Immunoprecipitation, RNAi validation and ELISA. Cross reactivity in Mouse and Rat reported by customer review.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
H00023435-M01 in the following applications:

Publications
Read Publications using
H00023435-M01 in the following applications:

  • 2 publications
  • IHC
    3 publications
  • 3 publications
  • IP
    2 publications
  • WB
    6 publications

Reactivity Notes

Cross reactivity in Mouse and Rat reported by customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TDP-43/TARDBP Antibody (2E2-D3)

  • ALS10
  • ALS10TAR DNA-binding protein-43
  • TAR DNA binding protein
  • TARDBP
  • TDP43
  • TDP-43
  • TDP-43TAR DNA-binding protein 43

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TDP-43/TARDBP Antibody (H00023435-M01)(147)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(2)
IHC
(3)
IHC-P
(3)
IP
(2)
WB
(6)
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Filter By Species
Human
(6)
Mouse
(4)
Rat
(1)
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Showing Publications 1 - 10 of 147. Show All 147 Publications.
Publications using H00023435-M01 Applications Species
Berson A, Sartoris A, Nativio R et al. TDP-43 Promotes Neurodegeneration by Impairing Chromatin Remodeling Curr. Biol. 2017 Nov 09 [PMID: 29153328] (Human) Human
Chang JC, Morton DB. Drosophila lines with mutant and wild type human TDP-43 replacing the endogenous gene reveals phosphorylation and ubiquitination in mutant lines in the absence of viability or lifespan defects. PLoS One 2017 Jul 7 [PMID: 28686708]
Moreno F, Indakoetxea B, Barandiaran M et al. The unexpected co-occurrence of GRN and MAPT p.A152T in Basque families: Clinical and pathological characteristics. PLoS One 2017 Jun 8 [PMID: 28594853]
Ash PEA, Stanford EA, Al Abdulatif A et al. Dioxins and related environmental contaminants increase TDP-43 levels. Mol Neurodegener 2017 May 5 [PMID: 28476168]
Koriath CAM, Bocchetta M, Brotherhood E et al. The clinical, neuroanatomical, and neuropathologic phenotype of TBK1-associated frontotemporal dementia: A longitudinal case report. Alzheimers & Dementia: Diagnosis, Assessment & Disease Monitoring 2016 Nov 3 [PMID: 28229125]
Wang W, Arakawa H, Wang L et al. Motor-Coordinative and Cognitive Dysfunction Caused by Mutant TDP-43 Could Be Reversed by Inhibiting Its Mitochondrial Localization. Mol Ther 2017 Jan 4 [PMID: 28129109]
van der Zee J, Gijselinck I, Van Mossevelde S et al. TBK1 Mutation Spectrum in an Extended European Patient Cohort With Frontotemporal Dementia and Amyotrophic Lateral Sclerosis. Hum Mutat 2016 Dec 23 [PMID: 28008748]
Redaelli V, Rossi G, Maderna E et al. Alzheimer neuropathology without frontotemporal lobar degeneration hallmarks (TAR DNA-binding protein 43 inclusions) in missense progranulin mutation Cys139Arg. Brain Pathol 2016 Dec 20 [PMID: 27997711]
Dutta K, Patel P, Rahimian R et al. Withania somnifera Reverses Transactive Response DNA Binding Protein 43 Proteinopathy in a Mouse Model of Amyotrophic Lateral Sclerosis/Frontotemporal Lobar Degeneration. Neurotherapeutics 2016 Dec 7 [PMID: 27928708]
Gelpi E, Hoftberger R, Graus F et al. Neuropathological criteria of anti-IgLON5-related tauopathy. Acta Neuropathol 2016 Jun 29 [PMID: 27358064]
Show All 147 Publications.

Reviews for TDP-43/TARDBP Antibody (H00023435-M01) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 review tested in 1 species: Mouse.

Reviews using H00023435-M01:
Filter by Applications
IHC-P
(1)
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin TDP-43/TARDBP H00023435-M01
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reviewed by:
Anonymous
IHC-P 10/02/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin
Pub Med ID22151540
FileView PDF
Western Blot TDP-43/TARDBP H00023435-M01
Enlarge
reviewed by:
Helen Chiang
WB Mouse 06/28/2012
View

Summary

ApplicationWestern Blot
Sample TestedMouse Brain Tissue
SpeciesMouse
Lot12076-S1
CommentsTransgenic mice expressing a C-terminal fragment of human TDP-43 gene

Comments

CommentsTransgenic mice expressing a C-terminal fragment of human TDP-43 gene

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WB Video Protocol
ICC/IF Video Protocol

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
10/02/2012
Application: IHC-P
Species:

Helen Chiang
06/28/2012
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TARDBP
Entrez
OMIM
Uniprot