Neuropilin-2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat ...read more
Simple Western lane view shows lysates of C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for ...read more
Neuropilin‑2 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Neuropilin-2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2
Gln23-Asp857 (Val809-Asp825 del)
Accession # O35276
Specificity
Detects mouse and rat Neuropilin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human Neuropilin-2 is observed, and less than 5% cross-reactivity with recombinant rat Neuropilin-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NRP2
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 4 Reviews rated 4
using
AF567 in the following applications:

Publications
Read Publications using
AF567 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuropilin-2 Antibody [Unconjugated]

  • MGC126574
  • neuropilin 2
  • Neuropilin2
  • Neuropilin-2
  • neuropilin-2a(22)
  • neuropilin-2b(0)
  • NP2
  • NPN 2
  • NPN2
  • PRO2714
  • receptor for VEGF165 and semaphorins class3
  • Vascular endothelial cell growth factor 165 receptor 2
  • vascular endothelial growth factor-165 receptor 2
  • VEGF1265R2
  • VEGF165R2neuropilin-2a(17)

Background

Neuropilin-1 (Npn-1, previously known as Neuropilin) and Npn-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind members of the class III secreted semaphorin subfamily which are implicated in repulsive axon guidance. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid sequence level, Npn-1and Npn-2 share 44% identity. Npn-1 and Npn-2 show different binding specificities for different members of the semaphorin family. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely nonoverlapping. Npn‑1 and Npn-2 are also expressed by endothelial and tumor cells and have been shown to be isoform-specific receptors for VEGF165. Npn‑1 was also reported to bind PlGF-2 and the VEGF-like protein from of virus NZ2.

  1. Fujisawa, H. and T. Kitsukawa (1998) Curr. Opin. Neurobiol. 8:587.
  2. Neufeld, G. et al. (1999) FASEB J. 13:9.
  3. Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neuropilin-2 Antibody (AF567)(12)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 7 applications: ICC, IHC, IHC Frozen, IHC Not Specified, Immunopreciptation, Neut, WB.


Filter By Application
ICC
(1)
IHC
(3)
IHC Frozen
(3)
IHC Not Specified
(1)
Immunopreciptation
(1)
Neut
(1)
WB
(2)
All Applications
Filter By Species
Mouse
(11)
Rat
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF567 Applications Species
M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program Nat Commun, 2018;9(1):1511. 2018 [PMID: 29666442] (IHC,, Mouse) IHC Mouse
F Tatin, E Renaud-Gab, AC Godet, F Hantelys, F Pujol, F Morfoisse, D Calise, F Viars, P Valet, B Masri, AC Prats, B Garmy-Susi Apelin modulates pathological remodeling of lymphatic endothelium after myocardial infarction JCI Insight, 2017;2(12):. 2017 [PMID: 28614788] (IHC,, Mouse) IHC Mouse
Rainer H Straub Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis J. Med. Microbiol., 2016;0(0):. 2016 [PMID: 27856318] (Neut,, Mouse) Neut Mouse
Boksik Cha Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves Genes Dev, 2016;30(12):1454-69. 2016 [PMID: 27313318] (IHC Frozen,, Mouse) IHC Frozen Mouse
MH Ulvmar, I Martinez-C, L Stanczuk, T Mäkinen Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins Genesis, 2016;0(0):. 2016 [PMID: 27060598] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Demyanenko G, Mohan V, Zhang X, Brennaman L, Dharbal K, Tran T, Manis P, Maness P Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling. J Neurosci, 2014;34(34):11274-87. 2014 [PMID: 25143608] (Immunopreciptation,, Mouse) Immunopreciptation Mouse
Baluk P, Adams A, Phillips K, Feng J, Hong Y, Brown M, McDonald D Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation. Am J Pathol, 2014;184(5):1577-92. 2014 [PMID: 24631179] (IHC,, Mouse) IHC Mouse
Bowles J, Secker G, Nguyen C, Kazenwadel J, Truong V, Frampton E, Curtis C, Skoczylas R, Davidson T, Miura N, Hong Y, Koopman P, Harvey N, Francois M Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo. Dev Biol, 2014;386(1):25-33. 2014 [PMID: 24361262] (IHC Frozen,, Mouse) IHC Frozen Mouse
Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J. Clin. Invest., 2010;120(5):1694-707. 2010 [PMID: 20364082] (WB,, Mouse) WB Mouse
Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice. J. Clin. Invest., 2008;118(6):2062-75. 2008 [PMID: 18483621] (IHC Frozen,, Mouse) IHC Frozen Mouse
Show All 12 Publications.

Reviews for Neuropilin-2 Antibody (AF567) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Mouse, Rat.

Reviews using AF567:
Filter by Applications
IF
(2)
WB
(2)
All Applications
Filter by Species
Mouse
(3)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Mouse 12/19/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHippocampal neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
IF Mouse 12/19/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHippocampal neurons and astrocytes
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsThree-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
WB Rat 12/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedRN22 cells
SpeciesRat

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
WB Mouse 12/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedPrimary hippocampal neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/

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FAQs for Neuropilin-2 Antibody (AF567) (0)

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Recent Reviews

4
5
1
4
2
3
1
2
0
1
0

 
Anonymous
12/19/2014
Application: IF
Species: Mouse

 
Anonymous
12/19/2014
Application: IF
Species: Mouse

 
Anonymous
12/19/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol NRP2
Uniprot