NELL1 Antibody (6A8) - Azide and BSA Free

Western Blot: NELL1 Antibody (6A8) [H00004745-M01] - Analysis of NELL1 expression in transfected 293T cell line by NELL1 monoclonal antibody (M01), clone 6A8.Lane 1: NELL1 transfected lysate(89.607 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: NELL1 Antibody (6A8) [H00004745-M01] - Analysis of monoclonal antibody to NELL1 on HeLa cell . Antibody concentration 10 ug/ml.

Immunoprecipitation: NELL1 Antibody (6A8) [H00004745-M01] - Analysis of NELL1 transfected lysate using anti-NELL1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NELL1 MaxPab rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 6A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NELL1 Antibody (6A8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NELL1 (NP_006148, 304 a.a. ~ 403 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CRRMSCPPLNCSPDSLPVHIAGQCCKVCRPKCIYGGKVLAEGQRILTKSCRECRGGVLVKITEMCPPLNCSEKDHILPENQCCRVCRGHNFCAEGPKCGE
• Localization: Secreted
• Specificity: NELL1 - NEL-like 1 (chicken)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NELL1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NELL1 Antibody (6A8) - Azide and BSA Free

• FLJ45906
• IDH3GL
• nel (chicken)-like 1
• NELL1
• NEL-like 1 (chicken)
• NEL-like protein 1
• Nel-related protein 1
• neural epidermal growth factor-like 1
• NRP1
• protein kinase C-binding protein NELL1

Background

This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF) -like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. An alternative splice variant has been described but its full-length sequence has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NELL1 Antibody (H00004745-M01)(2)

Publications using H00004745-M01

• Tian X, Wang Q, Wu J et al. Interaction of Nel-like molecule 1 with apoptosis related protein 3 with its influence on human dental pulp cells proliferation and differentiation into odontoblasts. Biochem Biophys Res Commun. 2019-08-12 [PMID: 31416616]
• Franke A, Hampe J, Rosenstiel P et al. Systematic association mapping identifies NELL1 as a novel IBD disease gene. PLoS ONE;2(1):e691. 2007-08-08 [PMID: 17684544]

Bioinformatics

• Gene Symbol: NELL1
• Entrez:
  • 4745(Human)
• OMIM:
  • 602319(Human)
• Uniprot:
  • NP_006148(Human)