Optineurin Antibody - Azide and BSA Free

Western Blot: Optineurin Antibody [H00010133-B01P] - Analysis of OPTN expression in human placenta.

Immunocytochemistry/Immunofluorescence: Optineurin Antibody [H00010133-B01P] - Analysis of purified antibody to OPTN on HeLa cell. Antibody at 10 ug/mL.

Western Blot: Optineurin Antibody [H00010133-B01P] - Analysis of OPTN expression in transfected 293T cell line by OPTN polyclonal antibody. Lane 1: OPTN transfected lysate (63.47 kDa). Lane 2: Non-transfected lysate.

Simple Western: Optineurin Antibody [H00010133-B01P] - This antibody (1:25 dilution) was used to probe THP-1 macrophage lysate (concentrations shown) by Simple Western. Simple Western image submitted by a verified customer review.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Optineurin Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Optineurin Antibody - Azide and BSA Free (H00010133-B01P) is a polyclonal antibody validated for use in WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: OPTN (NP_001008212.1, 1 a.a. - 577 a.a.) full-length human protein. MSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII
• Specificity: OPTN - optineurin,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: OPTN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Simple Western
  • Western Blot 1:500
• Application Notes: This Optineurin Antibody is validated for Simple Western from a verified customer review.
  See Simple Western Antibody Database for Simple Western validation: Tested in THP-1 macrophage lysate 0.2 mg/mL, 0.6 mg/mL and 1.2 mg/mL, separated by Size, antibody dilution of 1:25

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Optineurin Antibody - Azide and BSA Free

• ALS12
• FIP2
• FIP2FIP-2
• GLC1E
• GLC1Eglaucoma 1, open angle, E (adult-onset)
• HIP7
• HIP7ALS12
• Huntingtin interacting protein L
• Huntingtin yeast partner L
• Huntingtin-interacting protein 7
• Huntingtin-interacting protein L
• HYPL
• HYPLHIP-7
• NEMO-related protein
• NRP
• NRPE3-14.7K-interacting protein
• Optic neuropathy-inducing protein
• Optineurin
• OPTN
• TFIIIA-INTP
• Transcription factor IIIA-interacting protein
• transcrption factor IIIA-interacting protein
• tumor necrosis factor alpha-inducible cellular protein containing leucinezipper domains

Background

This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Optineurin Antibody (H00010133-B01P) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Simple Western Human 12/05/2019

Summary

• Application: Simple Western
• Sample Tested: THP-1 macrophage lysate
• Species: Human
• Lot: J3281

Bioinformatics

• Gene Symbol: OPTN
• Entrez:
  • 10133(Human)
• Uniprot:
  • NP_001008212.1(Human)