Olig2 [p Ser14, p Ser10, p Ser13] Antibody - Azide Free

Western Blot: OLIG2 [p Ser14, p Ser10, p Ser13] Antibody [NBP2-29523] - Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~ 32k form of the Olig2 protein phosphorylated at Ser10,13,14. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide), but not by the corresponding dephospho-peptide (not shown).

• Reactivity: Mu, Rt, Hu, Gp, Pm, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Olig2 [p Ser14, p Ser10, p Ser13] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser10,13,14 conjugated to KLH. Accession # Q13516
• Modification: p Ser14, p Ser10, p Ser13
• Marker: Oligodendrocyte Marker
• Specificity: Specific for endogenous levels of the ~32 kDa Olig2 phosphorylated at Ser10, Ser 13, and Ser14. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Species: Human (100%), Primate (100%), Zebrafish (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OLIG2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Olig2 [p Ser14, p Ser10, p Ser13] Antibody - Azide Free

• BHLHB1
• BHLHE19
• Class B basic helix-loop-helix protein 1
• Class E basic helix-loop-helix protein 19
• human protein kinase C-binding protein RACK17
• Olig2
• OLIGO2
• oligodendrocyte lineage transcription factor 2
• oligodendrocyte transcription factor 2
• oligodendrocyte-specific bHLH transcription factor 2
• PRKCBP2
• protein kinase C binding protein 2
• Protein kinase C-binding protein 2
• Protein kinase C-binding protein RACK17
• RACK17
• RACK17helix-loop-helix protein, class B, 1

Background

Olig2 is a well conserved bHLH transcription factor that shows both anti-neural functions and pro-neural functions at different stages in the formation of the oligodendrocyte lineage (Sun et al., 2011). Olig2 is expressed in 100% of the human diffuse gliomas irrespective of grade and required for intracranial tumor formation in a genetically relevant model of malignant glioma (Ligon et al., 2004; Ligon et al., 2007). A developmentally regulated triple serine motif at positions 10, 13 and 14 in the amino terminus is well conserved across species ranging from humans to zebrafish and is essential for Olig2 proliferative function in both normal and malignant neural progenitors (Sun et al., 2011). All three serine residues must be mutated to achieve a strong loss-of-function or gain-of-function phenotype, suggesting that the phosphorylation state of Olig2 represents a significant conformational change in the amino terminus (Sun et al., 2011).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OLIG2
• Entrez:
  • 10215(Human)
• Uniprot:
  • Q13516()