Neurogenin-2 Antibody (7G4)

• Reactivity: Hu, Rt
• Applications: IHC
• Clone: 7G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Neurogenin-2 Antibody (7G4) Summary

• Immunogen: E. coli-derived recombinant rat Neurogenin-2
• Specificity: Detect human and rat Neurogenin‑2.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEUROG2
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurogenin-2 Antibody (7G4)

• Atoh4
• BHLHA8
• bHLHa8MGC46562
• Class A basic helix-loop-helix protein 8
• Math4A
• NEUROG2
• neurogenin 2
• Neurogenin2
• Neurogenin-2
• NGN2
• ngn-2
• Protein atonal homolog 4

Background

Neurogenin-2 (NgN2) is a member of neuroD-related bHLH transcription factor family. It is required for neurogenesis and can be used as a marker for cells of neuronal lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurogenin-2 Antibody (MAB3314)(7)

Publications using MAB3314

• X Shi, Y Zhuang, Z Chen, M Xu, J Kuang, XL Sun, L Gao, X Kuang, H Zhang, W Li, SZH Wong, C Liu, L Liu, D Jiang, D Pei, Y Lin, QF Wu Hierarchical deployment of Tbx3 dictates the identity of hypothalamic KNDy neurons to control puberty onset Science Advances, 2022;8(46):eabq2987. 2022 [PMID: 36383654] (IHC, Mouse)
• YH Zhang, M Xu, X Shi, XL Sun, W Mu, H Wu, J Wang, S Li, P Su, L Gong, M He, M Yao, QF Wu Cascade diversification directs generation of neuronal diversity in the hypothalamus Cell Stem Cell, 2021;0(0):. 2021 [PMID: 33887179] (IHC, Mouse)
• S Cheng, T Liu, Y Hu, Y Xia, J Hou, C Huang, X Zou, J Liang, Y Stone Shi, Y Zheng, J Lu, G Chen Conditional inactivation of Pen-2 in the developing neocortex leads to rapid switch of apical progenitors to basal progenitors J. Neurosci., 2019;0(0):. 2019 [PMID: 30692224] (IHC-P, Mouse)
• B Connor, E Firmin, A McCaughey-, R Monk, K Lee, S Liot, J Geiger, C Rudolph, K Jones Conversion of adult human fibroblasts into neural precursor cells using chemically modified mRNA Heliyon, 2018;4(11):e00918. 2018 [PMID: 30450440] (ICC, Human)
• VM Jovanovic, A Salti, H Tilleman, K Zega, MM Jukic, H Zou, RH Friedel, N Prakash, S Blaess, F Edenhofer, C Brodski BMP/SMAD pathway promotes neurogenesis of midbrain dopaminergic neurons in vivo and in human induced pluripotent and neural stem cells J. Neurosci., 2018;0(0):. 2018 [PMID: 29321139] (IHC, Human)
• Maurer K, Riesenberg A, Brown N Notch signaling differentially regulates Atoh7 and Neurog2 in the distal mouse retina. Development, 2014;141(16):3243-54. 2014 [PMID: 25100656] (IHC, Mouse)
• Vue TY, Aaker J, Taniguchi A Characterization of progenitor domains in the developing mouse thalamus. J. Comp. Neurol., 2007;505(1):73-91. 2007 [PMID: 17729296] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: NEUROG2