SOX10 Antibody [Unconjugated]

Images

 
SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 10 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

SOX10 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX10
Met1-Ala118
Accession # P56693
Specificity
Detects human SOX10 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX10
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF2864 in the following applications:

Publications
Read Publications using
AF2864 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX10 Antibody [Unconjugated]

  • DOM
  • MGC15649
  • PCWH
  • SOX10
  • SRY (sex determining region Y)-box 10
  • SRY-related HMG-box gene 10
  • transcription factor SOX-10
  • WS4
  • WS4C
  • WS4mouse, human homolog of

Background

SOX10 belongs to the SOX family of transcription factors with diverse roles during development. In the central nervous system (CNS), SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-20486
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB100-56561
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NLS54
Species: Ca, Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
MAB2457
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow, WB
MAB5579
Species: Hu
Applications: ICC
AF3075
Species: Hu
Applications: ICC, Simple Western, WB
NB110-59723
Species: Hu, Mu, Po
Applications: ICC/IF, IHC, IHC-P, WB
AF482
Species: Mu
Applications: IHC, WB
AF2418
Species: Hu, Mu, Rt
Applications: ChIP, ICC, IHC, WB
NB600-717
Species: Bv, Ca, Ch, Fe, Gp, Hu, Ma, Mu, Po, Rb, Rt, Sh
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
NB100-1607
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IP, WB
NBP3-12898
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
212-GD/CF
Species: Hu
Applications: Bind, BA
NBP2-03886
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NBP2-67232
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-13349
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
AF682
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, WB
NB300-141
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-37463
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
AF2864
Species: Hu
Applications: WB, IHC, ICC/IF

Publications for SOX10 Antibody (AF2864)(39)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 9 applications: FACS, ICC, IHC, IHC-F, IHC-Fr, IHC-P, Immunoprecipitation, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
FACS
(1)
ICC
(14)
IHC
(13)
IHC-F
(1)
IHC-Fr
(4)
IHC-P
(2)
Immunoprecipitation
(1)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(16)
Mouse
(19)
Rat
(2)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using AF2864 Applications Species
KG Zyner, A Simeone, SM Flynn, C Doyle, G Marsico, S Adhikari, G Portella, D Tannahill, S Balasubram G-quadruplex DNA structures in human stem cells and differentiation Nature Communications, 2022;13(1):142. 2022 [PMID: 35013231] (ICC, Human) ICC Human
S Kohnke, S Buller, D Nuzzaci, K Ridley, B Lam, H Pivonkova, MA Bentsen, KM Alonge, C Zhao, J Tadross, S Holmqvist, T Shimizo, H Hathaway, H Li, W Macklin, MW Schwartz, WD Richardson, GSH Yeo, RJM Franklin, RT Karadottir, DH Rowitch, C Blouet Nutritional regulation of oligodendrocyte differentiation regulates perineuronal net remodeling in the median eminence Cell Reports, 2021;36(2):109362. 2021 [PMID: 34260928] (IHC, Mouse) IHC Mouse
P Duong, KH Ma, R Ramesh, JJ Moran, S Won, J Svaren H3K27 demethylases are dispensable for activation of Polycomb-regulated injury response genes in peripheral nerve The Journal of Biological Chemistry, 2021;297(1):100852. 2021 [PMID: 34090875] (IHC, Mouse) IHC Mouse
KP Kim, C Li, D Bunina, HW Jeong, J Ghelman, J Yoon, B Shin, H Park, DW Han, JB Zaugg, J Kim, T Kuhlmann, RH Adams, KM Noh, SA Goldman, HR Schöler Donor cell memory confers a metastable state of directly converted cells Cell Stem Cell, 2021;0(0):. 2021 [PMID: 33848472] (ICC, Mouse) ICC Mouse
P Kameneva, AV Artemov, ME Kastriti, L Faure, TK Olsen, J Otte, A Erickson, B Semsch, ER Andersson, M Ratz, J Frisén, AS Tischler, RR de Krijger, T Bouderliqu, N Akkuratova, M Vorontsova, O Gusev, K Fried, E Sundström, S Mei, P Kogner, N Baryawno, PV Kharchenko, I Adameyko Single-cell transcriptomics of human embryos identifies multiple sympathoblast lineages with potential implications for neuroblastoma origin Nature Genetics, 2021;0(0):. 2021 [PMID: 33833454] (IHC, Human) IHC Human
KJ Chang, I Agrawal, A Vainshtein, WY Ho, W Xin, G Tucker-Kel, K Susuki, E Peles, SC Ling, JR Chan TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells Elife, 2021;10(0):. 2021 [PMID: 33689679] (IHC, Mouse) IHC Mouse
I Paredes, JR Vieira, B Shah, CF Ramunno, J Dyckow, H Adler, M Richter, G Schermann, E Giannakour, L Schirmer, HG Augustin, C Ruiz de Al Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk Nature Neuroscience, 2021;0(0):. 2021 [PMID: 33510480] (IHC, Mouse) IHC Mouse
AJ Hulme, JR McArthur, S Maksour, S Miellet, L Ooi, DJ Adams, RK Finol-Urda, M Dottori Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons Frontiers in Cellular Neuroscience, 2020;14(0):600895. 2020 [PMID: 33362470] (ICC, Human) ICC Human
JA García-Leó, B García-Día, K Eggermont, L Cáceres-Pa, K Neyrinck, R Madeiro da, JC Dávila, A Baron-Van, A Gutiérrez, CM Verfaillie Generation of oligodendrocytes and establishment of an all-human myelinating platform from human pluripotent stem cells Nat Protoc, 2020;15(11):3716-3744. 2020 [PMID: 33097924] (ICC, Human) ICC Human
KC Allan, LR Hu, MA Scavuzzo, AR Morton, AS Gevorgyan, EF Cohn, BLL Clayton, IR Bederman, S Hung, CF Bartels, M Madhavan, PJ Tesar Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33091368] (Western Blot, Human) Western Blot Human
Show All 39 Publications.

Reviews for SOX10 Antibody (AF2864) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF2864:
Filter by Applications
Immunohistochemistry-Frozen
(1)
Immunohistochemistry-Paraffin
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen SOX10 AF2864
Enlarge
5
reviewed by:
Ricardo Vieira
Immunohistochemistry-Frozen Mouse 05/09/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedEmbryonic spinal cord
SpeciesMouse
LotVRY0516091
Immunohistochemistry-Paraffin SOX10 AF2864
Enlarge
5
reviewed by:
Verified Customer
Immunohistochemistry-Paraffin Mouse 02/26/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse
LotVRY081810C

Comments

CommentsAntigen retrieval: HIER, pH 6
Block: 1 hour in 2% normal donkey serum, 2 mg/ml BSA; 0.2% triton-x
Primary: 10 ug/ml in blocking buffer, overnight at 4C
Secondary: Donkey anti-goat DyLight 650, 1:300 for 1 hour

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOX10 Antibody (AF2864) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SOX10 Products

Bioinformatics Tool for SOX10 Antibody (AF2864)

Discover related pathways, diseases and genes to SOX10 Antibody (AF2864). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SOX10 Antibody (AF2864)

Discover more about diseases related to SOX10 Antibody (AF2864).
 

Pathways for SOX10 Antibody (AF2864)

View related products by pathway.

PTMs for SOX10 Antibody (AF2864)

Learn more about PTMs related to SOX10 Antibody (AF2864).

Blogs on SOX10

There are no specific blogs for SOX10, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Ricardo Vieira
05/09/2019
Application: Immunohistochemistry-Frozen
Species: Mouse

Verified Customer
02/26/2019
Application: Immunohistochemistry-Paraffin
Species: Mouse

Bioinformatics

Gene Symbol SOX10
Uniprot