SOX10 Antibody [Unconjugated]

Images

 
SOX10 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 3 ...read more
SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 10 µg/mL ...read more
SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 1 hour at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

SOX10 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX10
Met1-Ala118
Accession # P56693
Specificity
Detects human SOX10 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX10
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF2864 in the following applications:

Publications
Read Publications using
AF2864 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX10 Antibody [Unconjugated]

  • DOM
  • MGC15649
  • PCWH
  • SOX10
  • SRY (sex determining region Y)-box 10
  • SRY-related HMG-box gene 10
  • transcription factor SOX-10
  • WS4
  • WS4C
  • WS4mouse, human homolog of

Background

SOX10 belongs to the SOX family of transcription factors with diverse roles during development. In the central nervous system (CNS), SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX10 Antibody (AF2864)(50)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 11 applications: FACS, ICC, ICC/IF, IHC, IHC-F, IHC-Fr, IHC-P, IHC/IF, Immunoprecipitation, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
FACS
(1)
ICC
(15)
ICC/IF
(1)
IHC
(22)
IHC-F
(1)
IHC-Fr
(4)
IHC-P
(2)
IHC/IF
(1)
Immunoprecipitation
(1)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(20)
Mouse
(26)
Rat
(4)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using AF2864 Applications Species
Werner, L;Gliem, M;Rychlik, N;Pavic, G;Reiche, L;Kirchhoff, F;Silva Oliveira Junior, M;Gruchot, J;Meuth, SG;Küry, P;Göttle, P; A Novel Ex Vivo Model to Study Therapeutic Treatments for Myelin Repair following Ischemic Damage International journal of molecular sciences 2023-06-30 [PMID: 37446147] (IHC, Rat) IHC Rat
Huang, Z;Lin, J;Jiang, H;Lin, W;Huang, Z;Chen, J;Xiao, W;Lin, Q;Wang, J;Wen, S;Zhu, Q;Liu, J; Metformin promotes Schwann cell remyelination, preserves neural tissue and improves functional recovery after spinal cord injury Neuropeptides 2023-05-19 [PMID: 37236132] (IHC, Mouse) IHC Mouse
SV Fazal, C Mutschler, CZ Chen, M Turmaine, CY Chen, YP Hsueh, A Ibañez-Gra, A Loreto, A Casillas-B, H Cabedo, RJM Franklin, RA Barker, KR Monk, BJ Steventon, MP Coleman, JA Gomez-Sanc, P Arthur-Far SARM1 detection in myelinating glia: sarm1/Sarm1 is dispensable for PNS and CNS myelination in zebrafish and mice Frontiers in Cellular Neuroscience, 2023-04-05;17(0):1158388. 2023-04-05 [PMID: 37091921] (ICC, Mouse) ICC Mouse
Y Chen, C Wu, S Peng, D Guo, H Ouyang, Y Wei, R Ju, X Ding, Z Xie, C Liu Single-Cell Characterization of the Frizzled 5 (Fz5) Mutant Mouse and Human Persistent Fetal Vasculature (PFV) Investigative Ophthalmology & Visual Science, 2023-03-01;64(3):8. 2023-03-01 [PMID: 36867129] (IHC, Mouse) IHC Mouse
EF Cohn, BLL Clayton, M Madhavan, S Yacoub, Y Federov, K Paul-Fried, TJ Shafer, PJ Tesar Pervasive environmental chemicals impair oligodendrocyte development bioRxiv : the preprint server for biology, 2023-02-12;0(0):. 2023-02-12 [PMID: 36798415] (IHC, Human) IHC Human
Z Li, K Chen, Q Shao, H Lu, X Zhang, Y Pu, X Sun, H He, L Cao Nanoparticulate MgH2 ameliorates anxiety/depression-like behaviors in a mouse model of multiple sclerosis by regulating microglial polarization and oxidative stress Journal of Neuroinflammation, 2023-01-30;20(1):16. 2023-01-30 [PMID: 36710351] (ICC, IHC, Mouse) ICC, IHC Mouse
C Galante, N Marichal, FF Scarante, LM Ghayad, Y Shi, C Schuurmans, B Berninger, S Péron Enhanced proliferation of oligodendrocyte progenitor cells following retrovirus mediated Achaete-scute complex-like 1 overexpression in the postnatal cerebral cortex in vivo Frontiers in Neuroscience, 2022-12-02;16(0):919462. 2022-12-02 [PMID: 36532282] (IHC, Mouse) IHC Mouse
Ideno H, Imaizumi K, Shimada H, Sanosaka T, Nemoto A, Kohyama J, Okano H Human PSCs determine the competency of cerebral organoid differentiation via FGF signaling and epigenetic mechanisms. iScience, 2022-09-16;25(10):105140. 2022-09-16 [PMID: 36185382] (IHC, Human) IHC Human
P Karras, I Bordeu, J Pozniak, A Nowosad, C Pazzi, N Van Raemdo, E Landeloos, Y Van Herck, D Pedri, G Bervoets, S Makhzami, JH Khoo, B Pavie, J Lamote, O Marin-Beja, M Dewaele, H Liang, X Zhang, Y Hua, J Wouters, R Browaeys, G Bergers, Y Saeys, F Bosisio, J van den Oo, D Lambrechts, AK Rustgi, O Bechter, C Blanpain, BD Simons, F Rambow, JC Marine A cellular hierarchy in melanoma uncouples growth and metastasis Nature, 2022-09-21;0(0):. 2022-09-21 [PMID: 36131018] (IHC/IF, Human) IHC/IF Human
X Hu, N Agarwal, MD Zhang, P Ernfors, R Kuner, JR Nyengaard, P Karlsson Identification and quantification of nociceptive Schwann cells in mice with and without Streptozotocin-induced diabetes Journal of chemical neuroanatomy, 2022-06-06;0(0):102118. 2022-06-06 [PMID: 35680105] (IHC, Mouse) IHC Mouse
Show All 50 Publications.

Reviews for SOX10 Antibody (AF2864) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF2864:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen SOX10 AF2864
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 05/09/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedEmbryonic spinal cord
SpeciesMouse
LotVRY0516091
Immunohistochemistry-Paraffin SOX10 AF2864
Enlarge
5
reviewed by:
Kelli Jordan
IHC-P Mouse 02/26/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse
LotVRY081810C

Comments

CommentsAntigen retrieval: HIER, pH 6
Block: 1 hour in 2% normal donkey serum, 2 mg/ml BSA; 0.2% triton-x
Primary: 10 ug/ml in blocking buffer, overnight at 4C
Secondary: Donkey anti-goat DyLight 650, 1:300 for 1 hour

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SOX10 Antibody (AF2864) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SOX10 Antibody (AF2864)

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Diseases for SOX10 Antibody (AF2864)

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Pathways for SOX10 Antibody (AF2864)

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Blogs on SOX10

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
05/09/2019
Application: IHC-Fr
Species: Mouse

Kelli Jordan
02/26/2019
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol SOX10
Uniprot