ASCL1/Mash1 Antibody (BLR164J)

Immunoprecipitation:ASCL1/Mash1 Antibody [NBP3-14738] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from SHP-77 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiASCL1/MASH1 recombinant monoclonal antibody used for IP at 6 ul per reaction.ASCL1/MASH1 was also immunoprecipitated by a secondantibody against a different epitope of ASCL1/MASH1 For blotting immunoprecipitatedASCL1/MASH1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin:ASCL1/Mash1 Antibody [NBP3-14738] -ASCL1/MASH1 recombinant monoclonal antibody [BLR164J]. Secondary: HRP-conjugated goat antirabbit IgG (A120-501P). Substrate: DAB.

Western Blot: ASCL1/Mash1 Antibody [NBP3-14738] - Whole cell lysate (50 ug) from HEK293T, HeLa, SHP-77, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ASCL1/MASH1 recombinant monoclonal antibody [BLR164J] used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit antiActin recombinant monoclonal antibody [BLR057F].

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clone: BLR164J
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ASCL1/Mash1 Antibody (BLR164J) Summary

• Additional Information: Recombinant Monoclonal
• Immunogen: between 186 and 236 (C-term)
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ASCL1
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 to 1:500
  • Immunohistochemistry 1:100 to 1:500
  • Immunohistochemistry-Paraffin 1:100 to 1:500
  • Immunoprecipitation 6 µl/1 mg lysate
  • Western Blot 1:1,000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for ASCL1/Mash1 Antibody (BLR164J)

• achaete-scute complex (Drosophila) homolog-like 1
• achaete-scute complex homolog 1 (Drosophila)
• achaete-scute complex-like 1 (Drosophila)
• achaete-scute homolog 1
• ASCL1
• ASH1
• ASH-1
• ASH1MASH1
• BHLHA46
• bHLHa46achaete-scute complex-like 1
• Class A basic helix-loop-helix protein 46
• HASH1
• HASH1achaete scute protein
• Mash1

Background

MASH1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASCL1