Olig1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Olig1 Antibody [NBP1-31018] - OLIG1 antibody [C2C3], C-term detects OLIG1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: OLIG1 protein stained by OLIG1 antibody [C2C3], C-term (NBP1-31018).Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886].Blue: Fluoroshield with DAPI.

Immunohistochemistry: Olig1 Antibody [NBP1-31018] - Olig1 antibody [C2C3] detects Olig1 protein on adult mouse brain by immunohistochemical analysis. Sample: Frozen section of adult mouse brain. Red: Olig1 antibody [C2C3] (NBP1-31018) diluted at 1:200. Blue: DAPI

Western Blot: Olig1 Antibody [NBP1-31018] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE NBP1-31018 diluted at 1:1000

• Reactivity: Rt, Bv
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Olig1 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Olig1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OLIG1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Olig1 Antibody - BSA Free

• BHLHB6
• bHLHe21
• Class B basic helix-loop-helix protein 6
• Class E basic helix-loop-helix protein 21
• helix-loop-helix protein, class B, 6
• Olig1
• oligo1
• oligodendrocyte lineage transcription factor 1
• oligodendrocyte transcription factor 1
• oligodendrocyte-specific bHLH transcription factor 1

Background

Olig genes have been identified as the earliest known markers of oligodendrocyte lineage determination to date (Zhou et al., 2000). Olig1 is a transcription factor which promotes formation and maturation of oligodendrocytes, especially within the brain. It is expressed in the ventral spinal cord as early as 9.5 dpc and by 15.5 dpc, olig1 is dispersed throughout the gray matter. In the postnatal brain, it is present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Olig1 has been demonstrated as necessary in the repair of brain lesions in patients with multiple sclerosis (Arnett et al. 2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: OLIG1