Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 400 ng on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived rat MAG/Siglec-4a protein
  

  Rat MAG (Gly20-Pro516) Accession # P07722	DIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P07722
• N-terminal Sequence: Gly20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Mag
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 81 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF

• GMAsialic acid binding Ig-like lectin 4A
• MAG
• myelin associated glycoprotein
• myelin-associated glycoprotein
• sialic acid-binding immunoglobulin-like lectin 4A
• Siglec4a
• Siglec-4a
• S-MAG

Background

MAG (Myelin-Associated Glycoprotein), a type I transmembrane glycoprotein containing five Ig-like domains in its extracellular domain is an adhesion molecule belonging to the immunoglobin superfamily. Within this superfamily, MAG, CD22, CD33, Schwann cell myelin protein, and sialoadhesin which bind specifically to cell-surface glycan containing sialic acid residues define the I-type sialyl lectin subgroup, also called the sialoadhesin family. Sialoadhesins mediate diverse biological processes through recognition of specific sialyted glycans on cell surface. MAG is expressed on myelinating oligodenrocytes and Schwann cells, and preferentially recognizes alpha 2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG exists as two isoforms which differ in the sequence and length of the cytoplasmic tail. The large form (71 kDa) and small form (67 kDa) arise from alternative spliced mRNAs. Although MAG might encounter haematopoietic cells and lymphocytes under pathologic conditions, it would normally be expected to interact with neuronal cells. It has been shown that MAG promotes axonal growth from neonatal DRG neurons and embryonic spinal neurons, but is a potent inhibitor of axonal re-growth from adult DRG and postnatal cerebellar neurons. MAG plays an important role in the interaction between axons and myelin. A soluble form of MAG containing the extracellular domain is released from myelin in large quantities and identified in normal human tissues and in tissues from patients with neurological disorders. This soluble MAG might contribute to the lack of CNS neuron regeneration after injury.

1. Kelm, S. et al. (1994) Current Biology 4:965.
2. McKerracher, L. et al. (1994) Neuron 13:805.
3. Tang, S. et al. (1997) Molecular and Cellular Neuroscience 9:333.
4. Cai, D. et al. (1999) Neuron 22:89.

Publications for MAG/Siglec-4a (538-MG)(18)

Publications using 538-MG

• D Fernández-, FA Krapacher, A Andersson, CF Ibáñez, L Kisiswa MAG induces apoptosis in cerebellar granule neurons through p75NTR demarcating granule layer/white matter boundary Cell Death Dis, 2019;10(10):732. 2019 [PMID: 31570696] (Bioassay, Mouse)
• T Wang, B Li, Z Wang, X Yuan, C Chen, Y Zhang, Z Xia, X Wang, M Yu, W Tao, L Zhang, X Wang, Z Zhang, X Guo, G Ning, S Feng, X Chen miR-155-5p Promotes Dorsal Root Ganglion Neuron Axonal Growth in an Inhibitory Microenvironment via the cAMP/PKA Pathway Int. J. Biol. Sci., 2019;15(7):1557-1570. 2019 [PMID: 31337984] (Bioassay, Rat)
• F Liu, ML Gao, J Bai, YF Wang, XQ Li Inhibition of neurite outgrowth using commercial myelin associated glycoprotein-Fc in neuro-2a cells Neural Regen Res, 2018;13(11):1893-1899. 2018 [PMID: 30233061] (Bioassay, Rat)
• VSC Wong, C Picci, M Swift, M Levinson, D Willis, B Langley ?-Tubulin Acetyltransferase Is a Novel Target Mediating Neurite Growth Inhibitory Effects of Chondroitin Sulfate Proteoglycans and Myelin-Associated Glycoprotein eNeuro, 2018;5(1):. 2018 [PMID: 29497702] (Cell Culture, Mouse)
• Y Kawakami, Y Kurihara, Y Saito, Y Fujita, T Yamashita, K Takei The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor J. Neurosci., 2018;0(0):. 2018 [PMID: 29440387] (Bioassay, Mouse)
• Peterson S, Nguyen H, Mendez O, Anderson A Complement protein C1q modulates neurite outgrowth in vitro and spinal cord axon regeneration in vivo. J Neurosci, 2015;35(10):4332-49. 2015 [PMID: 25762679] (Bioassay, Mouse)
• Kurihara Y, Iketani M, Ito H, Nishiyama K, Sakakibara Y, Goshima Y, Takei K LOTUS suppresses axon growth inhibition by blocking interaction between Nogo receptor-1 and all four types of its ligand. Mol Cell Neurosci, 2014;61(0):211-8. 2014 [PMID: 25034269] (Bioassay, Chicken)
• Carlstrom LP, Hines JH, Henle SJ, Henley JR Bidirectional remodeling of beta1-integrin adhesions during chemotropic regulation of nerve growth. BMC Biol., 2011;9(0):82. 2011 [PMID: 22126462] (Xenopus)
• Matsushita H, Endo S, Kobayashi E, Sakamoto Y, Kobayashi K, Kitaguchi K, Kuroki K, Soderhall A, Maenaka K, Nakamura A, Strittmatter SM, Takai T Differential but competitive binding of Nogo protein and class i major histocompatibility complex (MHCI) to the PIR-B ectodomain provides an inhibition of cells. J. Biol. Chem., 2011;286(29):25739-47. 2011 [PMID: 21636572] (Surface Plasmon Resonance, Mouse)
• Robak LA, Venkatesh K, Lee H, Raiker SJ, Duan Y, Lee-Osbourne J, Hofer T, Mage RG, Rader C, Giger RJ Molecular basis of the interactions of the Nogo-66 receptor and its homolog NgR2 with myelin-associated glycoprotein: development of NgROMNI-Fc, a novel antagonist of CNS myelin inhibition. J. Neurosci., 2009;29(18):5768-83. 2009 [PMID: 19420245] (Bioassay, Rat)
• Williams G, Wood A, Williams EJ, Gao Y, Mercado ML, Katz A, Joseph-McCarthy D, Bates B, Ling HP, Aulabaugh A, Zaccardi J, Xie Y, Pangalos MN, Walsh FS, Doherty P Ganglioside inhibition of neurite outgrowth requires Nogo receptor function: identification of interaction sites and development of novel antagonists. J. Biol. Chem., 2008;283(24):16641-52. 2008 [PMID: 18411262] (Bioassay, Mouse)
• Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength. J. Neurosci., 2008;28(11):2753-65. 2008 [PMID: 18337405] (ICC, Human)
• Milward E, Kim KJ, Szklarczyk A, Nguyen T, Melli G, Nayak M, Deshpande D, Fitzsimmons C, Hoke A, Kerr D, Griffin JW, Calabresi PA, Conant K Cleavage of myelin associated glycoprotein by matrix metalloproteinases. J. Neuroimmunol., 2007;193(1):140-8. 2007 [PMID: 18063113] (Enzyme Assay Substrate, N/A)
• Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway. Biochem. Biophys. Res. Commun., 2007;365(2):375-80. 2007 [PMID: 17996195] (Bioassay, Rat)
• Willis DE, van Niekerk EA, Sasaki Y, Mesngon M, Merianda TT, Williams GG, Kendall M, Smith DS, Bassell GJ, Twiss JL Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs. J. Cell Biol., 2007;178(6):965-80. 2007 [PMID: 17785519] (ICC, Rat)
• Wright KT, El Masri W, Osman A, Roberts S, Chamberlain G, Ashton BA, Johnson WE Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A. Biochem. Biophys. Res. Commun., 2007;354(2):559-66. 2007 [PMID: 17234155] (Bioassay, Chicken)
• Hasegawa Y, Fujitani M, Hata K, Tohyama M, Yamagishi S, Yamashita T Promotion of axon regeneration by myelin-associated glycoprotein and Nogo through divergent signals downstream of Gi/G. J. Neurosci., 2004;24(30):6826-32. 2004 [PMID: 15282288] (Bioassay, Chicken)
• Wong ST, Henley JR, Kanning KC, Huang KH, Bothwell M, Poo MM A p75(NTR) and Nogo receptor complex mediates repulsive signaling by myelin-associated glycoprotein. Nat. Neurosci., 2002;5(12):1302-8. 2002 [PMID: 12426574] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Mag
• Uniprot:
  • P07722()