Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF


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Reactivity RtSpecies Glossary
Applications Bioactivity

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Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 400 ng on a nitrocellulose-coated microplate.
Mouse myeloma cell line, NS0-derived rat MAG/Siglec-4a protein
Accession # P07722
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
81 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
120 kDa, reducing conditions
Read Publications using
538-MG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF

  • GMAsialic acid binding Ig-like lectin 4A
  • MAG
  • myelin associated glycoprotein
  • myelin-associated glycoprotein
  • sialic acid-binding immunoglobulin-like lectin 4A
  • Siglec4a
  • Siglec-4a
  • S-MAG


MAG (Myelin-Associated Glycoprotein), a type I transmembrane glycoprotein containing five Ig-like domains in its extracellular domain is an adhesion molecule belonging to the immunoglobin superfamily. Within this superfamily, MAG, CD22, CD33, Schwann cell myelin protein, and sialoadhesin which bind specifically to cell-surface glycan containing sialic acid residues define the I-type sialyl lectin subgroup, also called the sialoadhesin family. Sialoadhesins mediate diverse biological processes through recognition of specific sialyted glycans on cell surface. MAG is expressed on myelinating oligodenrocytes and Schwann cells, and preferentially recognizes alpha 2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG exists as two isoforms which differ in the sequence and length of the cytoplasmic tail. The large form (71 kDa) and small form (67 kDa) arise from alternative spliced mRNAs. Although MAG might encounter haematopoietic cells and lymphocytes under pathologic conditions, it would normally be expected to interact with neuronal cells. It has been shown that MAG promotes axonal growth from neonatal DRG neurons and embryonic spinal neurons, but is a potent inhibitor of axonal re-growth from adult DRG and postnatal cerebellar neurons. MAG plays an important role in the interaction between axons and myelin. A soluble form of MAG containing the extracellular domain is released from myelin in large quantities and identified in normal human tissues and in tissues from patients with neurological disorders. This soluble MAG might contribute to the lack of CNS neuron regeneration after injury.

  1. Kelm, S. et al. (1994) Current Biology 4:965.
  2. McKerracher, L. et al. (1994) Neuron 13:805.
  3. Tang, S. et al. (1997) Molecular and Cellular Neuroscience 9:333.
  4. Cai, D. et al. (1999) Neuron 22:89.

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Publications for MAG/Siglec-4a (538-MG)(19)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Xenopus.

We have publications tested in 6 applications: Bioassay, Cell Culture, Enzyme Assay Substrate, ICC, In Vivo, Surface Plasmon Resonance.

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Enzyme Assay Substrate
In Vivo
Surface Plasmon Resonance
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using 538-MG Applications Species
W Pi, C Li, M Zhang, W Zhang, PX Zhang Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection Neural regeneration research, 2022-06-01;17(6):1343-1347. 2022-06-01 [PMID: 34782580] (In Vivo, Rat) In Vivo Rat
D Fernández-, FA Krapacher, A Andersson, CF Ibáñez, L Kisiswa MAG induces apoptosis in cerebellar granule neurons through p75NTR demarcating granule layer/white matter boundary Cell Death Dis, 2019-09-30;10(10):732. 2019-09-30 [PMID: 31570696] (Bioassay, Mouse) Bioassay Mouse
T Wang, B Li, Z Wang, X Yuan, C Chen, Y Zhang, Z Xia, X Wang, M Yu, W Tao, L Zhang, X Wang, Z Zhang, X Guo, G Ning, S Feng, X Chen miR-155-5p Promotes Dorsal Root Ganglion Neuron Axonal Growth in an Inhibitory Microenvironment via the cAMP/PKA Pathway Int. J. Biol. Sci., 2019-06-02;15(7):1557-1570. 2019-06-02 [PMID: 31337984] (Bioassay, Rat) Bioassay Rat
F Liu, ML Gao, J Bai, YF Wang, XQ Li Inhibition of neurite outgrowth using commercial myelin associated glycoprotein-Fc in neuro-2a cells Neural Regen Res, 2018-11-01;13(11):1893-1899. 2018-11-01 [PMID: 30233061] (Bioassay, Rat) Bioassay Rat
VSC Wong, C Picci, M Swift, M Levinson, D Willis, B Langley ?-Tubulin Acetyltransferase Is a Novel Target Mediating Neurite Growth Inhibitory Effects of Chondroitin Sulfate Proteoglycans and Myelin-Associated Glycoprotein eNeuro, 2018-02-28;5(1):. 2018-02-28 [PMID: 29497702] (Cell Culture, Mouse) Cell Culture Mouse
Y Kawakami, Y Kurihara, Y Saito, Y Fujita, T Yamashita, K Takei The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor J. Neurosci., 2018-02-09;0(0):. 2018-02-09 [PMID: 29440387] (Bioassay, Mouse) Bioassay Mouse
Peterson S, Nguyen H, Mendez O, Anderson A Complement protein C1q modulates neurite outgrowth in vitro and spinal cord axon regeneration in vivo. J Neurosci, 2015-03-11;35(10):4332-49. 2015-03-11 [PMID: 25762679] (Bioassay, Mouse) Bioassay Mouse
Kurihara Y, Iketani M, Ito H, Nishiyama K, Sakakibara Y, Goshima Y, Takei K LOTUS suppresses axon growth inhibition by blocking interaction between Nogo receptor-1 and all four types of its ligand. Mol Cell Neurosci, 2014-07-15;61(0):211-8. 2014-07-15 [PMID: 25034269] (Bioassay, Chicken) Bioassay Chicken
Carlstrom LP, Hines JH, Henle SJ, Henley JR Bidirectional remodeling of beta1-integrin adhesions during chemotropic regulation of nerve growth. BMC Biol., 2011-11-30;9(0):82. 2011-11-30 [PMID: 22126462] (Xenopus) Xenopus
Matsushita H, Endo S, Kobayashi E, Sakamoto Y, Kobayashi K, Kitaguchi K, Kuroki K, Soderhall A, Maenaka K, Nakamura A, Strittmatter SM, Takai T Differential but competitive binding of Nogo protein and class i major histocompatibility complex (MHCI) to the PIR-B ectodomain provides an inhibition of cells. J. Biol. Chem., 2011-06-02;286(29):25739-47. 2011-06-02 [PMID: 21636572] (Surface Plasmon Resonance, Mouse) Surface Plasmon Resonance Mouse
Show All 19 Publications.

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Gene Symbol Mag