Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF

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Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 400 ng on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived rat MAG/Siglec-4a protein
Rat MAG
(Gly20-Pro516)
Accession # P07722
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Mag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
81 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
120 kDa, reducing conditions
Publications
Read Publications using
538-MG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF

  • GMAsialic acid binding Ig-like lectin 4A
  • MAG
  • myelin associated glycoprotein
  • myelin-associated glycoprotein
  • sialic acid-binding immunoglobulin-like lectin 4A
  • Siglec4a
  • Siglec-4a
  • S-MAG

Background

MAG (Myelin-Associated Glycoprotein), a type I transmembrane glycoprotein containing five Ig-like domains in its extracellular domain is an adhesion molecule belonging to the immunoglobin superfamily. Within this superfamily, MAG, CD22, CD33, Schwann cell myelin protein, and sialoadhesin which bind specifically to cell-surface glycan containing sialic acid residues define the I-type sialyl lectin subgroup, also called the sialoadhesin family. Sialoadhesins mediate diverse biological processes through recognition of specific sialyted glycans on cell surface. MAG is expressed on myelinating oligodenrocytes and Schwann cells, and preferentially recognizes alpha 2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG exists as two isoforms which differ in the sequence and length of the cytoplasmic tail. The large form (71 kDa) and small form (67 kDa) arise from alternative spliced mRNAs. Although MAG might encounter haematopoietic cells and lymphocytes under pathologic conditions, it would normally be expected to interact with neuronal cells. It has been shown that MAG promotes axonal growth from neonatal DRG neurons and embryonic spinal neurons, but is a potent inhibitor of axonal re-growth from adult DRG and postnatal cerebellar neurons. MAG plays an important role in the interaction between axons and myelin. A soluble form of MAG containing the extracellular domain is released from myelin in large quantities and identified in normal human tissues and in tissues from patients with neurological disorders. This soluble MAG might contribute to the lack of CNS neuron regeneration after injury.

  1. Kelm, S. et al. (1994) Current Biology 4:965.
  2. McKerracher, L. et al. (1994) Neuron 13:805.
  3. Tang, S. et al. (1997) Molecular and Cellular Neuroscience 9:333.
  4. Cai, D. et al. (1999) Neuron 22:89.

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Publications for MAG/Siglec-4a (538-MG)(14)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, N/A, Xenopus.

We have publications tested in 4 applications: Bioassay, Enzyme Substrate, ICC, Surface Plasmon Resonance.


Filter By Application
Bioassay
(9)
Enzyme Substrate
(1)
ICC
(2)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(4)
Rat
(3)
Chicken
(3)
N/A
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 538-MG Applications Species
Y Kawakami, Y Kurihara, Y Saito, Y Fujita, T Yamashita, K Takei The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor J. Neurosci., 2018;0(0):. 2018 [PMID: 29440387] (Bioassay, Mouse) Bioassay Mouse
Peterson S, Nguyen H, Mendez O, Anderson A Complement protein C1q modulates neurite outgrowth in vitro and spinal cord axon regeneration in vivo. J Neurosci, 2015;35(10):4332-49. 2015 [PMID: 25762679] (Bioassay, Mouse) Bioassay Mouse
Kurihara Y, Iketani M, Ito H, Nishiyama K, Sakakibara Y, Goshima Y, Takei K LOTUS suppresses axon growth inhibition by blocking interaction between Nogo receptor-1 and all four types of its ligand. Mol Cell Neurosci, 2014;61(0):211-8. 2014 [PMID: 25034269] (Bioassay, Chicken) Bioassay Chicken
Carlstrom LP, Hines JH, Henle SJ, Henley JR Bidirectional remodeling of beta1-integrin adhesions during chemotropic regulation of nerve growth. BMC Biol., 2011;9(0):82. 2011 [PMID: 22126462] (Xenopus) Xenopus
Matsushita H, Endo S, Kobayashi E, Sakamoto Y, Kobayashi K, Kitaguchi K, Kuroki K, Soderhall A, Maenaka K, Nakamura A, Strittmatter SM, Takai T Differential but competitive binding of Nogo protein and class i major histocompatibility complex (MHCI) to the PIR-B ectodomain provides an inhibition of cells. J. Biol. Chem., 2011;286(29):25739-47. 2011 [PMID: 21636572] (Surface Plasmon Resonance, Mouse) Surface Plasmon Resonance Mouse
Robak LA, Venkatesh K, Lee H, Raiker SJ, Duan Y, Lee-Osbourne J, Hofer T, Mage RG, Rader C, Giger RJ Molecular basis of the interactions of the Nogo-66 receptor and its homolog NgR2 with myelin-associated glycoprotein: development of NgROMNI-Fc, a novel antagonist of CNS myelin inhibition. J. Neurosci., 2009;29(18):5768-83. 2009 [PMID: 19420245] (Bioassay, Rat) Bioassay Rat
Williams G, Wood A, Williams EJ, Gao Y, Mercado ML, Katz A, Joseph-McCarthy D, Bates B, Ling HP, Aulabaugh A, Zaccardi J, Xie Y, Pangalos MN, Walsh FS, Doherty P Ganglioside inhibition of neurite outgrowth requires Nogo receptor function: identification of interaction sites and development of novel antagonists. J. Biol. Chem., 2008;283(24):16641-52. 2008 [PMID: 18411262] (Bioassay, Mouse) Bioassay Mouse
Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength. J. Neurosci., 2008;28(11):2753-65. 2008 [PMID: 18337405] (ICC, Human) ICC Human
Milward E, Kim KJ, Szklarczyk A, Nguyen T, Melli G, Nayak M, Deshpande D, Fitzsimmons C, Hoke A, Kerr D, Griffin JW, Calabresi PA, Conant K Cleavage of myelin associated glycoprotein by matrix metalloproteinases. J. Neuroimmunol., 2007;193(1):140-8. 2007 [PMID: 18063113] (Enzyme Substrate, N/A) Enzyme Substrate N/A
Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway. Biochem. Biophys. Res. Commun., 2007;365(2):375-80. 2007 [PMID: 17996195] (Bioassay, Rat) Bioassay Rat
Show All 14 Publications.

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Bioinformatics

Gene Symbol Mag
Entrez
Uniprot