Nurim Antibody - Azide and BSA Free

Western Blot: Nurim Antibody [NBP2-99482] - Anti-Nurim rabbit polyclonal antibody at 1:2000 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa

Immunohistochemistry-Paraffin: Nurim Antibody [NBP2-99482] - Immunochemical staining of human Nurim in human stomach with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: Nurim Antibody [NBP2-99482] - Nurim was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 ul anti-Nurim rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Nurim rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa

• Reactivity: Hu, Po
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Nurim Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Nurim.
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NRM
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Nurim Antibody - Azide and BSA Free

• NRM29Nuclear envelope membrane protein
• Nuclear rim protein
• nurim (nuclear envelope membrane protein)
• nurim

Background

Nurim is an inner nuclear membrane (INM) protein that is tightly associated with the inner nuclear membrane. It was first isolated in a visual screen for nuclear envelope-localizing proteins. In subcellular fractionation, nurim remained extremely tightly bound to nuclear fractions. Unlike the known nuclear envelope (NE) membrane proteins, it is neither associated with nuclear pores, nor targeted like lamin-associated membrane proteins suggesting its localization to NE by a distinct mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NRM