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Human Coagulation Factor III/Tissue Factor Quantikine ELISA



Product Details

Reactivity HuSpecies Glossary
Applications ELISA

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Human Coagulation Factor III/Tissue Factor Quantikine ELISA Summary

The Quantikine Human Coagulation Factor III/Tissue Factor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Coagulation Factor III in cell culture supernates, cell lysates, plasma, and urine. It contains NS0-expressed recombinant human Coagulation Factor III and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Coagulatio...n Factor III showed linear curves that were p arallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Coagulation Factor III.
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Natural and recombinant human Coagulation Factor III
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
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DCF300 in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Coagulation Factor III/Tissue Factor Quantikine ELISA

  • CD142 antigen
  • CD142
  • coagulation factor III (thromboplastin, tissue factor)
  • Coagulation Factor III
  • F3
  • FLJ17960
  • TF
  • TFA
  • Thromboplastin
  • Tissue Factor


Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for Coagulation Factor III/Tissue Factor (DCF300)(25)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Capture.

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ELISA Capture
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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using DCF300 Applications Species
L Beckmann, J Mäder, M Voigtlaend, F Klingler, A Schulenkor, C Lehr, J Regenhardt, C Bokemeyer, W Ruf, C Rolling, F Langer Inhibition of protein disulfide isomerase with PACMA-31 regulates monocyte tissue factor through transcriptional and posttranscriptional mechanisms Thrombosis Research, 2022-09-30;220(0):48-59. 2022-09-30 [PMID: 36265413] (Human) Human
S Koizume, Y Miyagi Tissue factor in cancer-associated thromboembolism: possible mechanisms and clinical applications British Journal of Cancer, 2022-09-12;0(0):. 2022-09-12 [PMID: 36097177] (Human) Human
N Mackman, Y Hisada, SJ Archibald Tissue factor and its procoagulant activity on cancer-associated thromboembolism in pancreatic cancer: Comment by Mackman et al Cancer Science, 2022-02-07;0(0):. 2022-02-07 [PMID: 35132733] (Human) Human
VE Fiestas So, NR da Costa F, CF Dos Santos, G Corrêa, MDC Cipitelli, M Dornelas R, LJ de Souza, PV Damasco, RV da Cunha, FB Dos Santos, LM de Oliveir, EL de Azeredo Different Profiles of Cytokines, Chemokines and Coagulation Mediators Associated with Severity in Brazilian Patients Infected with Dengue Virus Viruses, 2021-09-08;13(9):. 2021-09-08 [PMID: 34578370] (Human) Human
L Beckmann, CC Rolling, M Voigtlände, J Mäder, F Klingler, A Schulenkor, C Lehr, C Bokemeyer, W Ruf, F Langer Bacitracin and Rutin Regulate Tissue Factor Production in Inflammatory Monocytes and Acute Myeloid Leukemia Blasts Cancers, 2021-08-04;13(16):. 2021-08-04 [PMID: 34439096] (Human) Human
T Mechelke, F Wittig, R Ramer, B Hinz Interleukin-1beta Induces Tissue Factor Expression in A549 Cells via EGFR-Dependent and -Independent Mechanisms International Journal of Molecular Sciences, 2021-06-21;22(12):. 2021-06-21 [PMID: 34205482] (Human) Human
S Yamakage, Y Oe, E Sato, K Okamoto, A Sekimoto, S Kumakura, H Sato, M Yoshida, T Nagasawa, M Miyazaki, S Ito, N Mackman, N Takahashi Myeloid cell-derived coagulation tissue factor is associated with renal tubular damage in mice fed an adenine diet Scientific Reports, 2021-06-09;11(1):12159. 2021-06-09 [PMID: 34108522] (Human) Human
E Nassar, N Hassan, EA El-Ghonaim, H Hassan, MS Abdullah, TV Rottke, L Kiesel, B Greve, SA Ibrahim, M Götte Syndecan-1 Promotes Angiogenesis in Triple-Negative Breast Cancer through the Prognostically Relevant Tissue Factor Pathway and Additional Angiogenic Routes Cancers, 2021-05-12;13(10):. 2021-05-12 [PMID: 34066023] (Human) Human
LE Landgrebe, CB Juhl, V Andersen, L Moitinho-S, C Bang, EM Bladbjerg Postprandial factor VII activation does not increase plasma concentrations of prothrombin fragment 1�+�2 in patients with morbid obesity Thromb. Res., 2020-09-01;196(0):260-267. 2020-09-01 [PMID: 32920297] (Human) Human
G Lano, M Laforêt, C Von Kotze, J Perrin, T Addi, P Brunet, S Poitevin, S Burtey, L Dou Aryl Hydrocarbon Receptor Activation and Tissue Factor Induction by Fluid Shear Stress and Indoxyl Sulfate in Endothelial Cells Int J Mol Sci, 2020-03-31;21(7):. 2020-03-31 [PMID: 32244284] (Human) Human
Show All 25 Publications.

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Product General Protocols

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FAQs for Coagulation Factor III/Tissue Factor (DCF300). (Showing 1 - 1 of 1 FAQs).

  1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
    • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

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Gene Symbol F3