Human Coagulation Factor III/Tissue Factor Quantikine ELISA

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human Coagulation Factor III/Tissue Factor Quantikine ELISA Summary

Background
The Quantikine Human Coagulation Factor III/Tissue Factor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Coagulation Factor III in cell culture supernates, cell lysates, plasma, and urine. It contains NS0-expressed recombinant human Coagulation Factor III and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Coagulatio...n Factor III showed linear curves that were p arallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Coagulation Factor III.
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Specificity
Natural and recombinant human Coagulation Factor III
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
F3

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DCF300 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Coagulation Factor III/Tissue Factor Quantikine ELISA

  • CD142 antigen
  • CD142
  • coagulation factor III (thromboplastin, tissue factor)
  • Coagulation Factor III
  • F3
  • FLJ17960
  • TF
  • TFA
  • Thromboplastin
  • Tissue Factor

Background

Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Coagulation Factor III/Tissue Factor (DCF300)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Capture.


Filter By Application
ELISA Capture
(1)
All Applications
Filter By Species
Human
(15)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using DCF300 Applications Species
M Wei, Y Liu, M Zheng, L Wang, F Ma, Y Qi, G Liu Upregulation of Protease-Activated Receptor 2 Promotes Proliferation and Migration of Human Vascular Smooth Muscle Cells (VSMCs) Med. Sci. Monit., 2019;25(0):8854-8862. 2019 [PMID: 31756174] (Human) Human
X Cheng, X Qiu, Y Liu, C Yuan, X Yang Trimethylamine N-oxide promotes tissue factor expression and activity in vascular endothelial cells: A new link between trimethylamine N-oxide and atherosclerotic thrombosis Thromb. Res., 2019;177(0):110-116. 2019 [PMID: 30875490] (Human) Human
NE Benelhaj, A Maraveyas, S Featherby, MEW Collier, MJ Johnson, C Ettelaie Alteration in endothelial permeability occurs in response to the activation of PAR2 by factor Xa but not directly by the TF-factor VIIa complex Thromb. Res., 2019;175(0):13-20. 2019 [PMID: 30677622] (Human) Human
A Baigger, D Eicke, Y Yuzefovych, D Pogozhykh, R Blasczyk, C Figueiredo Characterization of induced pluripotent stem cell-derived megakaryocyte lysates for potential regenerative applications J. Cell. Mol. Med., 2018;0(0):. 2018 [PMID: 29893509] (Human) Human
BA Hickey, A Cleves, R Alikhan, N Pugh, L Nokes, A Perera Can we use biomarkers of coagulation to predict which patients with foot and ankle injury will develop deep vein thrombosis? Foot Ankle Surg, 2017;0(0):. 2017 [PMID: 29409258] (ELISA Capture, Human) ELISA Capture Human
Alok A Khorana Tissue Factor As a Predictor of Recurrent Venous Thromboembolism in Malignancy: Biomarker Analyses of the CATCH Trial J. Clin. Oncol, 2016;0(0):JCO2016674564. 2016 [PMID: 28029329] (Human) Human
J Munrós Total circulating microparticle levels are increased in patients with deep infiltrating endometriosis Hum. Reprod, 2016;0(0):. 2016 [PMID: 27989989] (Human) Human
E Leal de Az, VE Solórzano, DB de Oliveir, CF Marinho, LJ de Souza, RV da Cunha, PV Damasco, CF Kubelka, LM de-Oliveir Increased circulating procoagulant and anticoagulant factors as TF and TFPI according to severity or infecting serotypes in human dengue infection Microbes Infect, 2017;19(1):62-68. 2017 [PMID: 27592310] (Human) Human
Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation Sci Rep, 2016;6(0):25171. 2016 [PMID: 27142573] (Human) Human
Bruneau S, Neel M, Roumenina L, Frimat M, Laurent L, Fremeaux-Bacchi V, Fakhouri F Loss of DGKepsilon induces endothelial cell activation and death independently of complement activation. Blood, 2015;125(6):1038-46. 2015 [PMID: 25498910] (Human) Human
Show All 15 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Coagulation Factor III/Tissue Factor (DCF300). (Showing 1 - 2 of 2 FAQs).

  1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
    • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.
  2. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DCF300

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Bioinformatics

Gene Symbol F3