SAP155 Antibody

Images

 
Independent Antibodies: Western Blot: SAP155 Antibody [NB100-55255] - Detection of Human SF3b155/SAP155 on HeLa whole cell lysate using NB100-55255. SF3b155/SAP155 was also immunoprecipitated by rabbit ...read more
Genetic Strategies: Knockdown Validated: SAP155 Antibody [NB100-55255] - In vivo Smarce1-binding sites were detected by chromatin immunoprecipitation using a SMARCE1 antibody. Cropped gel images representing ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

SAP155 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human splicing factor 3b, subunit 1, 155kDa using the numbering given in entry NP_036565.2 (GeneID 23451).
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SF3B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Simple Western
  • Western Blot 1:2000-1:10000
Application Notes
Use in SW reported in secitific publication PMID: 32427851
Publications
Read Publication using
NB100-55255 in the following applications:

  • SW
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAP155 Antibody

  • Hsh155
  • pre-mRNA processing 10
  • pre-mRNA splicing factor SF3b, 155 kDa subunit
  • Pre-mRNA-splicing factor SF3b 155 kDa subunit
  • Prp10
  • PRPF10
  • SAP 155
  • SAP155splicing factor 3b, subunit 1, 155kD
  • SF3B155
  • SF3b155PRP10
  • spliceosome associated protein 155
  • Spliceosome-associated protein 155
  • splicing factor 3B subunit 1
  • splicing factor 3b, subunit 1, 155kDa

Background

SAP155 is a subunit of the splicing factor SF3B required for SF3B155 is a subunit of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) that functions in pre-mRNA splicing. SF3B155 together with splicing factor 3a binds pre-mRNA upstream of the branchpoint sequence to anchor U2 snRNP to the pre-mRNA. SF3B155 is also a component of the U12-type splicesome that is involved in the splicing of a rare class of nuclear pre-mRNA introns.'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of the SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. It may also be involved in the assembly of the 'E' complex. SAP155 also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SAP155 Antibody (NB100-55255)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: SW.


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SW
(1)
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Showing Publication 1 - 1 of 1.
Publication using NB100-55255 Applications Species
Li C, Xie W, Rosenblum JS et al. Somatic SF3B1 hotspot mutation in prolactinomas Nat Commun May 19 2020 [PMID: 32427851] (SW, Human) SW Human

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Bioinformatics

Gene Symbol SF3B1
Entrez
Uniprot